Exploring heterotrophic bacterial diversity of Antarctic samples through cultivation
The microbial diversity on Antarctica is largely under-explored. As part of the AMBIO-project that aims to explore the factors affecting bacterial distribution patterns in Antarctica, nine samples from different regions were investigated. They include two samples from the Belgian Base site at Utstei...
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Universiteit Gent. Faculteit Wetenschappen
2009
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ftunivgent:oai:archive.ugent.be:936624 2023-06-11T04:06:08+02:00 Exploring heterotrophic bacterial diversity of Antarctic samples through cultivation Peeters, Karolien Verleyen, Elie Ertz, Damien Hodgson, Dominique Willems, Anne 2009 https://biblio.ugent.be/publication/936624 http://hdl.handle.net/1854/LU-936624 eng eng Universiteit Gent. Faculteit Wetenschappen https://biblio.ugent.be/publication/936624 http://hdl.handle.net/1854/LU-936624 Doctoraatssymposium Faculteit Wetenschappen UGent, Abstracts Biology and Life Sciences cultivation heterotrophic bacterial diversity Antarctica conference info:eu-repo/semantics/conferenceObject info:eu-repo/semantics/publishedVersion 2009 ftunivgent 2023-05-10T22:51:20Z The microbial diversity on Antarctica is largely under-explored. As part of the AMBIO-project that aims to explore the factors affecting bacterial distribution patterns in Antarctica, nine samples from different regions were investigated. They include two samples from the Belgian Base site at Utsteinen: BB50 from a green microbial/algal mat on gravel from the nunatak and BB115 from a black mat on gravel and rock debris from a frozen lake on the south side of the nunatak Utsteinen; two samples from the Trans-Antarctic Mountains: TM2 from a cyanobacterial mat at the bottom of Forlidas Pond (Pensacola Mountains) and TM4 from Lundström Lake (Shackleton Mountains); a littoral sample PQ1 from Pourquoi-Pas Lake (Pourquoi-Pas Island); samples LA3 (Langhovde Peninsula), SK5 (Skarvsness Peninsula) and WO10 (West Ongul Island) from three lakes at Lützow-Holm Bay, Syowa and sample SO6 from the Schirmacher Oasis. Samples were investigated with a culture-dependent approach. Dilution series were plated on four media (MA, R2A, R2A/10 and PYGV) and incubated at three temperatures (4, 15 and 20ºC). Different types of colonies were picked up for all conditions. As a fast way of screening the isolates, the genotypic typing method rep-PCR fingerprinting was used. Cluster analysis of fingerprint patterns using Bionumerics software revealed a number of clusters (cut-off level 80%) of similar strains and a number of separate isolates. Representatives of each rep-cluster and the separate isolates were used in partial 16S rDNA sequencing to obtain a first approximate identification. For the different samples, between 338 (BB115) and 669 (WO10) isolates were obtained and purified. Rep-PCR fingerprinting analysis resulted in 33 (LA3) to 101 (BB50) clusters and 54 (BB115) to 151 (PQ1) separate isolates (excluding samples WO10 and SO6 for which the analysis is not finished yet). The preliminary results of the 16S rDNA sequencing show a large diversity, distributed over the major phylogenetic groups. The BB samples and TM4 are dominated by ... Conference Object Antarc* Antarctic Antarctica Ongul Island Pourquoi Pas Island Ghent University Academic Bibliography Antarctic Shackleton Pourquoi Pas ENVELOPE(135.783,135.783,-66.083,-66.083) Pourquoi-Pas ENVELOPE(-67.450,-67.450,-67.700,-67.700) Pourquoi-Pas? ENVELOPE(135.750,135.750,-66.200,-66.200) Ongul ENVELOPE(39.533,39.533,-69.017,-69.017) Ongul Island ENVELOPE(39.533,39.533,-69.017,-69.017) Langhovde ENVELOPE(39.733,39.733,-69.217,-69.217) Lützow-Holm Bay ENVELOPE(38.000,38.000,-69.500,-69.500) Frozen Lake ENVELOPE(76.108,76.108,-69.415,-69.415) Pensacola Mountains ENVELOPE(-58.000,-58.000,-83.500,-83.500) Utsteinen ENVELOPE(23.342,23.342,-71.955,-71.955) Pourquoi Pas Island ENVELOPE(-67.500,-67.500,-67.683,-67.683) Forlidas Pond ENVELOPE(-51.318,-51.318,-82.453,-82.453) |
institution |
Open Polar |
collection |
Ghent University Academic Bibliography |
op_collection_id |
ftunivgent |
language |
English |
topic |
Biology and Life Sciences cultivation heterotrophic bacterial diversity Antarctica |
spellingShingle |
Biology and Life Sciences cultivation heterotrophic bacterial diversity Antarctica Peeters, Karolien Verleyen, Elie Ertz, Damien Hodgson, Dominique Willems, Anne Exploring heterotrophic bacterial diversity of Antarctic samples through cultivation |
topic_facet |
Biology and Life Sciences cultivation heterotrophic bacterial diversity Antarctica |
description |
The microbial diversity on Antarctica is largely under-explored. As part of the AMBIO-project that aims to explore the factors affecting bacterial distribution patterns in Antarctica, nine samples from different regions were investigated. They include two samples from the Belgian Base site at Utsteinen: BB50 from a green microbial/algal mat on gravel from the nunatak and BB115 from a black mat on gravel and rock debris from a frozen lake on the south side of the nunatak Utsteinen; two samples from the Trans-Antarctic Mountains: TM2 from a cyanobacterial mat at the bottom of Forlidas Pond (Pensacola Mountains) and TM4 from Lundström Lake (Shackleton Mountains); a littoral sample PQ1 from Pourquoi-Pas Lake (Pourquoi-Pas Island); samples LA3 (Langhovde Peninsula), SK5 (Skarvsness Peninsula) and WO10 (West Ongul Island) from three lakes at Lützow-Holm Bay, Syowa and sample SO6 from the Schirmacher Oasis. Samples were investigated with a culture-dependent approach. Dilution series were plated on four media (MA, R2A, R2A/10 and PYGV) and incubated at three temperatures (4, 15 and 20ºC). Different types of colonies were picked up for all conditions. As a fast way of screening the isolates, the genotypic typing method rep-PCR fingerprinting was used. Cluster analysis of fingerprint patterns using Bionumerics software revealed a number of clusters (cut-off level 80%) of similar strains and a number of separate isolates. Representatives of each rep-cluster and the separate isolates were used in partial 16S rDNA sequencing to obtain a first approximate identification. For the different samples, between 338 (BB115) and 669 (WO10) isolates were obtained and purified. Rep-PCR fingerprinting analysis resulted in 33 (LA3) to 101 (BB50) clusters and 54 (BB115) to 151 (PQ1) separate isolates (excluding samples WO10 and SO6 for which the analysis is not finished yet). The preliminary results of the 16S rDNA sequencing show a large diversity, distributed over the major phylogenetic groups. The BB samples and TM4 are dominated by ... |
format |
Conference Object |
author |
Peeters, Karolien Verleyen, Elie Ertz, Damien Hodgson, Dominique Willems, Anne |
author_facet |
Peeters, Karolien Verleyen, Elie Ertz, Damien Hodgson, Dominique Willems, Anne |
author_sort |
Peeters, Karolien |
title |
Exploring heterotrophic bacterial diversity of Antarctic samples through cultivation |
title_short |
Exploring heterotrophic bacterial diversity of Antarctic samples through cultivation |
title_full |
Exploring heterotrophic bacterial diversity of Antarctic samples through cultivation |
title_fullStr |
Exploring heterotrophic bacterial diversity of Antarctic samples through cultivation |
title_full_unstemmed |
Exploring heterotrophic bacterial diversity of Antarctic samples through cultivation |
title_sort |
exploring heterotrophic bacterial diversity of antarctic samples through cultivation |
publisher |
Universiteit Gent. Faculteit Wetenschappen |
publishDate |
2009 |
url |
https://biblio.ugent.be/publication/936624 http://hdl.handle.net/1854/LU-936624 |
long_lat |
ENVELOPE(135.783,135.783,-66.083,-66.083) ENVELOPE(-67.450,-67.450,-67.700,-67.700) ENVELOPE(135.750,135.750,-66.200,-66.200) ENVELOPE(39.533,39.533,-69.017,-69.017) ENVELOPE(39.533,39.533,-69.017,-69.017) ENVELOPE(39.733,39.733,-69.217,-69.217) ENVELOPE(38.000,38.000,-69.500,-69.500) ENVELOPE(76.108,76.108,-69.415,-69.415) ENVELOPE(-58.000,-58.000,-83.500,-83.500) ENVELOPE(23.342,23.342,-71.955,-71.955) ENVELOPE(-67.500,-67.500,-67.683,-67.683) ENVELOPE(-51.318,-51.318,-82.453,-82.453) |
geographic |
Antarctic Shackleton Pourquoi Pas Pourquoi-Pas Pourquoi-Pas? Ongul Ongul Island Langhovde Lützow-Holm Bay Frozen Lake Pensacola Mountains Utsteinen Pourquoi Pas Island Forlidas Pond |
geographic_facet |
Antarctic Shackleton Pourquoi Pas Pourquoi-Pas Pourquoi-Pas? Ongul Ongul Island Langhovde Lützow-Holm Bay Frozen Lake Pensacola Mountains Utsteinen Pourquoi Pas Island Forlidas Pond |
genre |
Antarc* Antarctic Antarctica Ongul Island Pourquoi Pas Island |
genre_facet |
Antarc* Antarctic Antarctica Ongul Island Pourquoi Pas Island |
op_source |
Doctoraatssymposium Faculteit Wetenschappen UGent, Abstracts |
op_relation |
https://biblio.ugent.be/publication/936624 http://hdl.handle.net/1854/LU-936624 |
_version_ |
1768377911352492032 |