Bacterial diversity of Antarctic samples through cultivation
The microbial diversity on Antarctica is largely unknown. As part of the AMBIO-project, five samples from different regions were investigated. Two gravel samples BB50 and BB115 (Utsteinen, Dronning Maud Land) and three littoral samples TM2, TM4 (Transantarctic Mountains) and PQ1 (Pourquoi-pas Island...
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ftunivgent:oai:archive.ugent.be:936606 2023-06-11T04:06:08+02:00 Bacterial diversity of Antarctic samples through cultivation Peeters, Karolien Verleyen, Elie Ertz, Damien Hodgson, Dominique Willems, Anne 2008 https://biblio.ugent.be/publication/936606 http://hdl.handle.net/1854/LU-936606 eng eng https://biblio.ugent.be/publication/936606 http://hdl.handle.net/1854/LU-936606 Polar and Alpine Microbiology, 3rd International conference, Abstracts Biology and Life Sciences Pourquoi-Pas Island cultivation Transantarctic Mountains Belgian Antarctic research centre heterotrophic bacterial diversity conference info:eu-repo/semantics/conferenceObject info:eu-repo/semantics/publishedVersion 2008 ftunivgent 2023-05-10T22:51:20Z The microbial diversity on Antarctica is largely unknown. As part of the AMBIO-project, five samples from different regions were investigated. Two gravel samples BB50 and BB115 (Utsteinen, Dronning Maud Land) and three littoral samples TM2, TM4 (Transantarctic Mountains) and PQ1 (Pourquoi-pas Island) were used. Samples were investigated with a culture-dependent approach. Dilution series were plated on four media and incubated at three temperatures. After obtaining pure cultures, rep-PCR technique was used for dereplication. Representatives of each rep-cluster were used in partial 16S rDNA sequencing to obtain a first approximate identification. The preliminary results show a large diversity, distributed over the major phylogenetic groups. The BB samples, TM4 and PQ1 were dominated by isolates of the class of Actinobacteria, most TM2 isolates belong to the Firmicutes class. In addition, for all samples, isolates from the Bacteroidetes and Proteobacteria classes were recovered. Despite the large diversity and the fact that some samples originate from the same general area, only little overlap between the samples was observed. Some of the isolated clusters show low similarity values with neighbouring sequences in the EMBL-database and may represent new species or even new genera. Conference Object Antarc* Antarctic Antarctica Dronning Maud Land Pourquoi Pas Island Ghent University Academic Bibliography Antarctic Dronning Maud Land Transantarctic Mountains Pourquoi Pas ENVELOPE(135.783,135.783,-66.083,-66.083) Pourquoi-Pas ENVELOPE(-67.450,-67.450,-67.700,-67.700) Pourquoi-Pas? ENVELOPE(135.750,135.750,-66.200,-66.200) Utsteinen ENVELOPE(23.342,23.342,-71.955,-71.955) Pourquoi Pas Island ENVELOPE(-67.500,-67.500,-67.683,-67.683) |
institution |
Open Polar |
collection |
Ghent University Academic Bibliography |
op_collection_id |
ftunivgent |
language |
English |
topic |
Biology and Life Sciences Pourquoi-Pas Island cultivation Transantarctic Mountains Belgian Antarctic research centre heterotrophic bacterial diversity |
spellingShingle |
Biology and Life Sciences Pourquoi-Pas Island cultivation Transantarctic Mountains Belgian Antarctic research centre heterotrophic bacterial diversity Peeters, Karolien Verleyen, Elie Ertz, Damien Hodgson, Dominique Willems, Anne Bacterial diversity of Antarctic samples through cultivation |
topic_facet |
Biology and Life Sciences Pourquoi-Pas Island cultivation Transantarctic Mountains Belgian Antarctic research centre heterotrophic bacterial diversity |
description |
The microbial diversity on Antarctica is largely unknown. As part of the AMBIO-project, five samples from different regions were investigated. Two gravel samples BB50 and BB115 (Utsteinen, Dronning Maud Land) and three littoral samples TM2, TM4 (Transantarctic Mountains) and PQ1 (Pourquoi-pas Island) were used. Samples were investigated with a culture-dependent approach. Dilution series were plated on four media and incubated at three temperatures. After obtaining pure cultures, rep-PCR technique was used for dereplication. Representatives of each rep-cluster were used in partial 16S rDNA sequencing to obtain a first approximate identification. The preliminary results show a large diversity, distributed over the major phylogenetic groups. The BB samples, TM4 and PQ1 were dominated by isolates of the class of Actinobacteria, most TM2 isolates belong to the Firmicutes class. In addition, for all samples, isolates from the Bacteroidetes and Proteobacteria classes were recovered. Despite the large diversity and the fact that some samples originate from the same general area, only little overlap between the samples was observed. Some of the isolated clusters show low similarity values with neighbouring sequences in the EMBL-database and may represent new species or even new genera. |
format |
Conference Object |
author |
Peeters, Karolien Verleyen, Elie Ertz, Damien Hodgson, Dominique Willems, Anne |
author_facet |
Peeters, Karolien Verleyen, Elie Ertz, Damien Hodgson, Dominique Willems, Anne |
author_sort |
Peeters, Karolien |
title |
Bacterial diversity of Antarctic samples through cultivation |
title_short |
Bacterial diversity of Antarctic samples through cultivation |
title_full |
Bacterial diversity of Antarctic samples through cultivation |
title_fullStr |
Bacterial diversity of Antarctic samples through cultivation |
title_full_unstemmed |
Bacterial diversity of Antarctic samples through cultivation |
title_sort |
bacterial diversity of antarctic samples through cultivation |
publishDate |
2008 |
url |
https://biblio.ugent.be/publication/936606 http://hdl.handle.net/1854/LU-936606 |
long_lat |
ENVELOPE(135.783,135.783,-66.083,-66.083) ENVELOPE(-67.450,-67.450,-67.700,-67.700) ENVELOPE(135.750,135.750,-66.200,-66.200) ENVELOPE(23.342,23.342,-71.955,-71.955) ENVELOPE(-67.500,-67.500,-67.683,-67.683) |
geographic |
Antarctic Dronning Maud Land Transantarctic Mountains Pourquoi Pas Pourquoi-Pas Pourquoi-Pas? Utsteinen Pourquoi Pas Island |
geographic_facet |
Antarctic Dronning Maud Land Transantarctic Mountains Pourquoi Pas Pourquoi-Pas Pourquoi-Pas? Utsteinen Pourquoi Pas Island |
genre |
Antarc* Antarctic Antarctica Dronning Maud Land Pourquoi Pas Island |
genre_facet |
Antarc* Antarctic Antarctica Dronning Maud Land Pourquoi Pas Island |
op_source |
Polar and Alpine Microbiology, 3rd International conference, Abstracts |
op_relation |
https://biblio.ugent.be/publication/936606 http://hdl.handle.net/1854/LU-936606 |
_version_ |
1768377911704813568 |