Effect of pluronic block polymers and N-acetylcysteine culture media additives on growth rate and fatty acid composition of six marine microalgae species
The efficiency of microalgal biomass production is a determining factor for the economic competitiveness of microalgae-based industries. N-acetylcysteine (NAC) and pluronic block polymers are two compounds of interest as novel culture media constituents because of their respective protective propert...
Published in: | Applied Microbiology and Biotechnology |
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2021
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Online Access: | https://biblio.ugent.be/publication/8738696 http://hdl.handle.net/1854/LU-8738696 https://doi.org/10.1007/s00253-021-11147-8 https://biblio.ugent.be/publication/8738696/file/8738697 |
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ftunivgent:oai:archive.ugent.be:8738696 2023-06-11T04:11:10+02:00 Effect of pluronic block polymers and N-acetylcysteine culture media additives on growth rate and fatty acid composition of six marine microalgae species Sauvage, Justine Wikfors, Gary H. Li, Xiaoxu Gluis, Mark Nevejan, Nancy Sabbe, Koen Joyce, Alyssa 2021 application/pdf https://biblio.ugent.be/publication/8738696 http://hdl.handle.net/1854/LU-8738696 https://doi.org/10.1007/s00253-021-11147-8 https://biblio.ugent.be/publication/8738696/file/8738697 eng eng https://biblio.ugent.be/publication/8738696 http://hdl.handle.net/1854/LU-8738696 http://dx.doi.org/10.1007/s00253-021-11147-8 https://biblio.ugent.be/publication/8738696/file/8738697 Creative Commons Attribution 4.0 International Public License (CC-BY 4.0) info:eu-repo/semantics/openAccess APPLIED MICROBIOLOGY AND BIOTECHNOLOGY ISSN: 0175-7598 ISSN: 1432-0614 Agriculture and Food Sciences Marine microalgae Growth medium formulation Biochemical composition Live (aquaculture) feed Pluronic block polymers N-acetylcysteine journalArticle info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2021 ftunivgent https://doi.org/10.1007/s00253-021-11147-8 2023-04-19T22:10:36Z The efficiency of microalgal biomass production is a determining factor for the economic competitiveness of microalgae-based industries. N-acetylcysteine (NAC) and pluronic block polymers are two compounds of interest as novel culture media constituents because of their respective protective properties against oxidative stress and shear-stress-induced cell damage. Here we quantify the effect of NAC and two pluronic (F127 and F68) culture media additives upon the culture productivity of six marine microalgal species of relevance to the aquaculture industry (four diatoms-Chaetoceros calcitrans, Chaetoceros muelleri, Skeletonema costatum, and Thalassiosira pseudonana; two haptophytes-Tisochrysis lutea and Pavlova salina). Algal culture performance in response to the addition of NAC and pluronic, singly or combined, is dosage- and species-dependent. Combined NAC and pluronic F127 algal culture media additives resulted in specific growth rate increases of 38%, 16%, and 24% for C. calcitrans, C. muelleri, and P. salina, respectively. Enhanced culture productivity for strains belonging to the genus Chaetoceros was paired with an similar to 27% increase in stationary-phase cell density. For some of the species examined, culture media enrichments with NAC and pluronic resulted in increased omega-3-fatty acid content of the algal biomass. Larval development (i.e., growth and survival) of the Pacific oyster (Crassostrea gigas) was not changed when fed a mixture of microalgae grown in NAC- and F127-supplemented culture medium. Based upon these results, we propose that culture media enrichment with NAC and pluronic F127 is an effective and easily adopted approach to increase algal productivity and enhance the nutritional quality of marine microalgal strains commonly cultured for live-feed applications in aquaculture. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Ghent University Academic Bibliography Pacific Applied Microbiology and Biotechnology 105 5 2139 2156 |
institution |
Open Polar |
collection |
Ghent University Academic Bibliography |
op_collection_id |
ftunivgent |
language |
English |
topic |
Agriculture and Food Sciences Marine microalgae Growth medium formulation Biochemical composition Live (aquaculture) feed Pluronic block polymers N-acetylcysteine |
spellingShingle |
Agriculture and Food Sciences Marine microalgae Growth medium formulation Biochemical composition Live (aquaculture) feed Pluronic block polymers N-acetylcysteine Sauvage, Justine Wikfors, Gary H. Li, Xiaoxu Gluis, Mark Nevejan, Nancy Sabbe, Koen Joyce, Alyssa Effect of pluronic block polymers and N-acetylcysteine culture media additives on growth rate and fatty acid composition of six marine microalgae species |
topic_facet |
Agriculture and Food Sciences Marine microalgae Growth medium formulation Biochemical composition Live (aquaculture) feed Pluronic block polymers N-acetylcysteine |
description |
The efficiency of microalgal biomass production is a determining factor for the economic competitiveness of microalgae-based industries. N-acetylcysteine (NAC) and pluronic block polymers are two compounds of interest as novel culture media constituents because of their respective protective properties against oxidative stress and shear-stress-induced cell damage. Here we quantify the effect of NAC and two pluronic (F127 and F68) culture media additives upon the culture productivity of six marine microalgal species of relevance to the aquaculture industry (four diatoms-Chaetoceros calcitrans, Chaetoceros muelleri, Skeletonema costatum, and Thalassiosira pseudonana; two haptophytes-Tisochrysis lutea and Pavlova salina). Algal culture performance in response to the addition of NAC and pluronic, singly or combined, is dosage- and species-dependent. Combined NAC and pluronic F127 algal culture media additives resulted in specific growth rate increases of 38%, 16%, and 24% for C. calcitrans, C. muelleri, and P. salina, respectively. Enhanced culture productivity for strains belonging to the genus Chaetoceros was paired with an similar to 27% increase in stationary-phase cell density. For some of the species examined, culture media enrichments with NAC and pluronic resulted in increased omega-3-fatty acid content of the algal biomass. Larval development (i.e., growth and survival) of the Pacific oyster (Crassostrea gigas) was not changed when fed a mixture of microalgae grown in NAC- and F127-supplemented culture medium. Based upon these results, we propose that culture media enrichment with NAC and pluronic F127 is an effective and easily adopted approach to increase algal productivity and enhance the nutritional quality of marine microalgal strains commonly cultured for live-feed applications in aquaculture. |
format |
Article in Journal/Newspaper |
author |
Sauvage, Justine Wikfors, Gary H. Li, Xiaoxu Gluis, Mark Nevejan, Nancy Sabbe, Koen Joyce, Alyssa |
author_facet |
Sauvage, Justine Wikfors, Gary H. Li, Xiaoxu Gluis, Mark Nevejan, Nancy Sabbe, Koen Joyce, Alyssa |
author_sort |
Sauvage, Justine |
title |
Effect of pluronic block polymers and N-acetylcysteine culture media additives on growth rate and fatty acid composition of six marine microalgae species |
title_short |
Effect of pluronic block polymers and N-acetylcysteine culture media additives on growth rate and fatty acid composition of six marine microalgae species |
title_full |
Effect of pluronic block polymers and N-acetylcysteine culture media additives on growth rate and fatty acid composition of six marine microalgae species |
title_fullStr |
Effect of pluronic block polymers and N-acetylcysteine culture media additives on growth rate and fatty acid composition of six marine microalgae species |
title_full_unstemmed |
Effect of pluronic block polymers and N-acetylcysteine culture media additives on growth rate and fatty acid composition of six marine microalgae species |
title_sort |
effect of pluronic block polymers and n-acetylcysteine culture media additives on growth rate and fatty acid composition of six marine microalgae species |
publishDate |
2021 |
url |
https://biblio.ugent.be/publication/8738696 http://hdl.handle.net/1854/LU-8738696 https://doi.org/10.1007/s00253-021-11147-8 https://biblio.ugent.be/publication/8738696/file/8738697 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY ISSN: 0175-7598 ISSN: 1432-0614 |
op_relation |
https://biblio.ugent.be/publication/8738696 http://hdl.handle.net/1854/LU-8738696 http://dx.doi.org/10.1007/s00253-021-11147-8 https://biblio.ugent.be/publication/8738696/file/8738697 |
op_rights |
Creative Commons Attribution 4.0 International Public License (CC-BY 4.0) info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1007/s00253-021-11147-8 |
container_title |
Applied Microbiology and Biotechnology |
container_volume |
105 |
container_issue |
5 |
container_start_page |
2139 |
op_container_end_page |
2156 |
_version_ |
1768386071083614208 |