More bone with less minerals? The effects of dietary phosphorus on the post-cranial skeleton in zebrafish
Dietary phosphorus (P) is essential for bone mineralisation in vertebrates. P deficiency can cause growth retardation, osteomalacia and bone deformities, both in teleosts and in mammals. Conversely, excess P supply can trigger soft tissue calcification and bone hypermineralisation. This study uses a...
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ftunivgent:oai:archive.ugent.be:8737469 2023-06-11T04:10:20+02:00 More bone with less minerals? The effects of dietary phosphorus on the post-cranial skeleton in zebrafish Cotti, Silvia Huysseune, Ann Koppe, Wolfgang Rucklin, Martin Marone, Federica Woelfel, Eva M. Fiedler, Imke A. K. Busse, Bjoern Forlino, Antonella Witten, Paul Eckhard 2020 application/pdf https://biblio.ugent.be/publication/8737469 http://hdl.handle.net/1854/LU-8737469 https://doi.org/10.3390/ijms21155429 https://biblio.ugent.be/publication/8737469/file/8763402 eng eng https://biblio.ugent.be/publication/8737469 http://hdl.handle.net/1854/LU-8737469 http://dx.doi.org/10.3390/ijms21155429 https://biblio.ugent.be/publication/8737469/file/8763402 Creative Commons Attribution 4.0 International Public License (CC-BY 4.0) info:eu-repo/semantics/openAccess INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES ISSN: 1422-0067 Biology and Life Sciences mineralisation bone formation vertebral column vertebral body fusion collagen SALMON SALMO-SALAR JUVENILE ATLANTIC SALMON TELEOST FISH VERTEBRAL ABNORMALITIES COMPARATIVE VIEW AXIAL SKELETON DANIO-RERIO X-RAY MINERALIZATION PHOSPHATE journalArticle info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2020 ftunivgent https://doi.org/10.3390/ijms21155429 2023-05-10T22:53:59Z Dietary phosphorus (P) is essential for bone mineralisation in vertebrates. P deficiency can cause growth retardation, osteomalacia and bone deformities, both in teleosts and in mammals. Conversely, excess P supply can trigger soft tissue calcification and bone hypermineralisation. This study uses a wide range of complementary techniques (X-rays, histology, TEM, synchrotron X-ray tomographic microscopy, nanoindentation) to describe in detail the effects of dietary P on the zebrafish skeleton, after two months of administering three different diets: 0.5% (low P, LP), 1.0% (regular P, RP), and 1.5% (high P, HP) total P content. LP zebrafish display growth retardation and hypomineralised bones, albeit without deformities. LP zebrafish increase production of non-mineralised bone matrix, and osteoblasts have enlarged endoplasmic reticulum cisternae, indicative for increased collagen synthesis. The HP diet promotes growth, high mineralisation, and stiffness but causes vertebral centra fusions. Structure and arrangement of bone matrix collagen fibres are not influenced by dietary P in all three groups. In conclusion, low dietary P content stimulates the formation of non-mineralised bone without inducing malformations. This indicates that bone formation and mineralisation are uncoupled. In contrast, high dietary P content promotes mineralisation and vertebral body fusions. This new zebrafish model is a useful tool to understand the mechanisms underlying osteomalacia and abnormal mineralisation, due to underlying variations in dietary P levels. Article in Journal/Newspaper Atlantic salmon Salmo salar Ghent University Academic Bibliography International Journal of Molecular Sciences 21 15 5429 |
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Open Polar |
collection |
Ghent University Academic Bibliography |
op_collection_id |
ftunivgent |
language |
English |
topic |
Biology and Life Sciences mineralisation bone formation vertebral column vertebral body fusion collagen SALMON SALMO-SALAR JUVENILE ATLANTIC SALMON TELEOST FISH VERTEBRAL ABNORMALITIES COMPARATIVE VIEW AXIAL SKELETON DANIO-RERIO X-RAY MINERALIZATION PHOSPHATE |
spellingShingle |
Biology and Life Sciences mineralisation bone formation vertebral column vertebral body fusion collagen SALMON SALMO-SALAR JUVENILE ATLANTIC SALMON TELEOST FISH VERTEBRAL ABNORMALITIES COMPARATIVE VIEW AXIAL SKELETON DANIO-RERIO X-RAY MINERALIZATION PHOSPHATE Cotti, Silvia Huysseune, Ann Koppe, Wolfgang Rucklin, Martin Marone, Federica Woelfel, Eva M. Fiedler, Imke A. K. Busse, Bjoern Forlino, Antonella Witten, Paul Eckhard More bone with less minerals? The effects of dietary phosphorus on the post-cranial skeleton in zebrafish |
topic_facet |
Biology and Life Sciences mineralisation bone formation vertebral column vertebral body fusion collagen SALMON SALMO-SALAR JUVENILE ATLANTIC SALMON TELEOST FISH VERTEBRAL ABNORMALITIES COMPARATIVE VIEW AXIAL SKELETON DANIO-RERIO X-RAY MINERALIZATION PHOSPHATE |
description |
Dietary phosphorus (P) is essential for bone mineralisation in vertebrates. P deficiency can cause growth retardation, osteomalacia and bone deformities, both in teleosts and in mammals. Conversely, excess P supply can trigger soft tissue calcification and bone hypermineralisation. This study uses a wide range of complementary techniques (X-rays, histology, TEM, synchrotron X-ray tomographic microscopy, nanoindentation) to describe in detail the effects of dietary P on the zebrafish skeleton, after two months of administering three different diets: 0.5% (low P, LP), 1.0% (regular P, RP), and 1.5% (high P, HP) total P content. LP zebrafish display growth retardation and hypomineralised bones, albeit without deformities. LP zebrafish increase production of non-mineralised bone matrix, and osteoblasts have enlarged endoplasmic reticulum cisternae, indicative for increased collagen synthesis. The HP diet promotes growth, high mineralisation, and stiffness but causes vertebral centra fusions. Structure and arrangement of bone matrix collagen fibres are not influenced by dietary P in all three groups. In conclusion, low dietary P content stimulates the formation of non-mineralised bone without inducing malformations. This indicates that bone formation and mineralisation are uncoupled. In contrast, high dietary P content promotes mineralisation and vertebral body fusions. This new zebrafish model is a useful tool to understand the mechanisms underlying osteomalacia and abnormal mineralisation, due to underlying variations in dietary P levels. |
format |
Article in Journal/Newspaper |
author |
Cotti, Silvia Huysseune, Ann Koppe, Wolfgang Rucklin, Martin Marone, Federica Woelfel, Eva M. Fiedler, Imke A. K. Busse, Bjoern Forlino, Antonella Witten, Paul Eckhard |
author_facet |
Cotti, Silvia Huysseune, Ann Koppe, Wolfgang Rucklin, Martin Marone, Federica Woelfel, Eva M. Fiedler, Imke A. K. Busse, Bjoern Forlino, Antonella Witten, Paul Eckhard |
author_sort |
Cotti, Silvia |
title |
More bone with less minerals? The effects of dietary phosphorus on the post-cranial skeleton in zebrafish |
title_short |
More bone with less minerals? The effects of dietary phosphorus on the post-cranial skeleton in zebrafish |
title_full |
More bone with less minerals? The effects of dietary phosphorus on the post-cranial skeleton in zebrafish |
title_fullStr |
More bone with less minerals? The effects of dietary phosphorus on the post-cranial skeleton in zebrafish |
title_full_unstemmed |
More bone with less minerals? The effects of dietary phosphorus on the post-cranial skeleton in zebrafish |
title_sort |
more bone with less minerals? the effects of dietary phosphorus on the post-cranial skeleton in zebrafish |
publishDate |
2020 |
url |
https://biblio.ugent.be/publication/8737469 http://hdl.handle.net/1854/LU-8737469 https://doi.org/10.3390/ijms21155429 https://biblio.ugent.be/publication/8737469/file/8763402 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES ISSN: 1422-0067 |
op_relation |
https://biblio.ugent.be/publication/8737469 http://hdl.handle.net/1854/LU-8737469 http://dx.doi.org/10.3390/ijms21155429 https://biblio.ugent.be/publication/8737469/file/8763402 |
op_rights |
Creative Commons Attribution 4.0 International Public License (CC-BY 4.0) info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.3390/ijms21155429 |
container_title |
International Journal of Molecular Sciences |
container_volume |
21 |
container_issue |
15 |
container_start_page |
5429 |
_version_ |
1768384688816128000 |