Subfunctionalization of paralog transcription factors contributes to regulation of alkaloid pathway branch choice in Catharanthus roseus
Catharanthus roseus produces a diverse range of specialized metabolites of the monoterpenoid indole alkaloid (MIA) class in a heavily branched pathway. Recent great progress in identification of MIA biosynthesis genes revealed that the different pathway branch genes are expressed in a highly cell ty...
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ftunivgent:oai:archive.ugent.be:8712660 2023-06-11T04:15:46+02:00 Subfunctionalization of paralog transcription factors contributes to regulation of alkaloid pathway branch choice in Catharanthus roseus Colinas Martinez, Maite Pollier, Jacob Vaneechoutte, Dries Malat, Deniz Schweizer, Fabian De Milde, Liesbeth De Clercq, Rebecca Guedes, Joana G. Martínez-Cortés, Teresa Hidalgo, Francisco Sottomayor, Mariana Vandepoele, Klaas Goossens, Alain 2021 application/pdf https://biblio.ugent.be/publication/8712660 http://hdl.handle.net/1854/LU-8712660 https://doi.org/10.3389/fpls.2021.687406 https://biblio.ugent.be/publication/8712660/file/8712664 eng eng https://biblio.ugent.be/publication/8712660 http://hdl.handle.net/1854/LU-8712660 http://dx.doi.org/10.3389/fpls.2021.687406 https://biblio.ugent.be/publication/8712660/file/8712664 Creative Commons Attribution 4.0 International Public License (CC-BY 4.0) info:eu-repo/semantics/openAccess FRONTIERS IN PLANT SCIENCE ISSN: 1664-462X Biology and Life Sciences Plant Science AP2 ERF transcription factor bHLH transcription factor Catharanthus roseus Madagascar periwinkle monoterpenoid indole alkaloids transcription factor gene clusters transcription factor paralogs synergistic regulation CUTICULAR WAX BIOSYNTHESIS TRITERPENE SAPONIN BIOSYNTHESIS 7-DEOXYLOGANETIC ACID NICOTINE BIOSYNTHESIS MOLECULAR-CLONING DRUG COMPONENTS GENE-EXPRESSION RESPONSE FACTOR journalArticle info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2021 ftunivgent https://doi.org/10.3389/fpls.2021.687406 2023-04-19T22:10:08Z Catharanthus roseus produces a diverse range of specialized metabolites of the monoterpenoid indole alkaloid (MIA) class in a heavily branched pathway. Recent great progress in identification of MIA biosynthesis genes revealed that the different pathway branch genes are expressed in a highly cell type- and organ-specific and stress-dependent manner. This implies a complex control by specific transcription factors (TFs), only partly revealed today. We generated and mined a comprehensive compendium of publicly available C. roseus transcriptome data for MIA pathway branch-specific TFs. Functional analysis was performed through extensive comparative gene expression analysis and profiling of over 40 MIA metabolites in the C. roseus flower petal expression system. We identified additional members of the known BIS and ORCA regulators. Further detailed study of the ORCA TFs suggests subfunctionalization of ORCA paralogs in terms of target gene-specific regulation and synergistic activity with the central jasmonate response regulator MYC2. Moreover, we identified specific amino acid residues within the ORCA DNA-binding domains that contribute to the differential regulation of some MIA pathway branches. Our results advance our understanding of TF paralog specificity for which, despite the common occurrence of closely related paralogs in many species, comparative studies are scarce. Article in Journal/Newspaper Orca Ghent University Academic Bibliography Frontiers in Plant Science 12 |
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Ghent University Academic Bibliography |
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language |
English |
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Biology and Life Sciences Plant Science AP2 ERF transcription factor bHLH transcription factor Catharanthus roseus Madagascar periwinkle monoterpenoid indole alkaloids transcription factor gene clusters transcription factor paralogs synergistic regulation CUTICULAR WAX BIOSYNTHESIS TRITERPENE SAPONIN BIOSYNTHESIS 7-DEOXYLOGANETIC ACID NICOTINE BIOSYNTHESIS MOLECULAR-CLONING DRUG COMPONENTS GENE-EXPRESSION RESPONSE FACTOR |
spellingShingle |
Biology and Life Sciences Plant Science AP2 ERF transcription factor bHLH transcription factor Catharanthus roseus Madagascar periwinkle monoterpenoid indole alkaloids transcription factor gene clusters transcription factor paralogs synergistic regulation CUTICULAR WAX BIOSYNTHESIS TRITERPENE SAPONIN BIOSYNTHESIS 7-DEOXYLOGANETIC ACID NICOTINE BIOSYNTHESIS MOLECULAR-CLONING DRUG COMPONENTS GENE-EXPRESSION RESPONSE FACTOR Colinas Martinez, Maite Pollier, Jacob Vaneechoutte, Dries Malat, Deniz Schweizer, Fabian De Milde, Liesbeth De Clercq, Rebecca Guedes, Joana G. Martínez-Cortés, Teresa Hidalgo, Francisco Sottomayor, Mariana Vandepoele, Klaas Goossens, Alain Subfunctionalization of paralog transcription factors contributes to regulation of alkaloid pathway branch choice in Catharanthus roseus |
topic_facet |
Biology and Life Sciences Plant Science AP2 ERF transcription factor bHLH transcription factor Catharanthus roseus Madagascar periwinkle monoterpenoid indole alkaloids transcription factor gene clusters transcription factor paralogs synergistic regulation CUTICULAR WAX BIOSYNTHESIS TRITERPENE SAPONIN BIOSYNTHESIS 7-DEOXYLOGANETIC ACID NICOTINE BIOSYNTHESIS MOLECULAR-CLONING DRUG COMPONENTS GENE-EXPRESSION RESPONSE FACTOR |
description |
Catharanthus roseus produces a diverse range of specialized metabolites of the monoterpenoid indole alkaloid (MIA) class in a heavily branched pathway. Recent great progress in identification of MIA biosynthesis genes revealed that the different pathway branch genes are expressed in a highly cell type- and organ-specific and stress-dependent manner. This implies a complex control by specific transcription factors (TFs), only partly revealed today. We generated and mined a comprehensive compendium of publicly available C. roseus transcriptome data for MIA pathway branch-specific TFs. Functional analysis was performed through extensive comparative gene expression analysis and profiling of over 40 MIA metabolites in the C. roseus flower petal expression system. We identified additional members of the known BIS and ORCA regulators. Further detailed study of the ORCA TFs suggests subfunctionalization of ORCA paralogs in terms of target gene-specific regulation and synergistic activity with the central jasmonate response regulator MYC2. Moreover, we identified specific amino acid residues within the ORCA DNA-binding domains that contribute to the differential regulation of some MIA pathway branches. Our results advance our understanding of TF paralog specificity for which, despite the common occurrence of closely related paralogs in many species, comparative studies are scarce. |
format |
Article in Journal/Newspaper |
author |
Colinas Martinez, Maite Pollier, Jacob Vaneechoutte, Dries Malat, Deniz Schweizer, Fabian De Milde, Liesbeth De Clercq, Rebecca Guedes, Joana G. Martínez-Cortés, Teresa Hidalgo, Francisco Sottomayor, Mariana Vandepoele, Klaas Goossens, Alain |
author_facet |
Colinas Martinez, Maite Pollier, Jacob Vaneechoutte, Dries Malat, Deniz Schweizer, Fabian De Milde, Liesbeth De Clercq, Rebecca Guedes, Joana G. Martínez-Cortés, Teresa Hidalgo, Francisco Sottomayor, Mariana Vandepoele, Klaas Goossens, Alain |
author_sort |
Colinas Martinez, Maite |
title |
Subfunctionalization of paralog transcription factors contributes to regulation of alkaloid pathway branch choice in Catharanthus roseus |
title_short |
Subfunctionalization of paralog transcription factors contributes to regulation of alkaloid pathway branch choice in Catharanthus roseus |
title_full |
Subfunctionalization of paralog transcription factors contributes to regulation of alkaloid pathway branch choice in Catharanthus roseus |
title_fullStr |
Subfunctionalization of paralog transcription factors contributes to regulation of alkaloid pathway branch choice in Catharanthus roseus |
title_full_unstemmed |
Subfunctionalization of paralog transcription factors contributes to regulation of alkaloid pathway branch choice in Catharanthus roseus |
title_sort |
subfunctionalization of paralog transcription factors contributes to regulation of alkaloid pathway branch choice in catharanthus roseus |
publishDate |
2021 |
url |
https://biblio.ugent.be/publication/8712660 http://hdl.handle.net/1854/LU-8712660 https://doi.org/10.3389/fpls.2021.687406 https://biblio.ugent.be/publication/8712660/file/8712664 |
genre |
Orca |
genre_facet |
Orca |
op_source |
FRONTIERS IN PLANT SCIENCE ISSN: 1664-462X |
op_relation |
https://biblio.ugent.be/publication/8712660 http://hdl.handle.net/1854/LU-8712660 http://dx.doi.org/10.3389/fpls.2021.687406 https://biblio.ugent.be/publication/8712660/file/8712664 |
op_rights |
Creative Commons Attribution 4.0 International Public License (CC-BY 4.0) info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.3389/fpls.2021.687406 |
container_title |
Frontiers in Plant Science |
container_volume |
12 |
_version_ |
1768372860658647040 |