Light regulation of LHCX genes in the benthic diatom Seminavis robusta
Intertidal benthic diatoms experience a highly variable light regime, which especially challenges these organisms to cope with excess light energy during low tide. Non-photochemical quenching of chlorophyll fluorescence (NPQ) is one of the most rapid mechanisms diatoms possess to dissipate excess en...
Published in: | Frontiers in Marine Science |
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Online Access: | https://biblio.ugent.be/publication/8660083 http://hdl.handle.net/1854/LU-8660083 https://doi.org/10.3389/fmars.2020.00192 https://biblio.ugent.be/publication/8660083/file/8660085 |
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ftunivgent:oai:archive.ugent.be:8660083 2023-06-11T04:16:38+02:00 Light regulation of LHCX genes in the benthic diatom Seminavis robusta Blommaert, Lander Vancaester, Emmelien Huysman, Marie Osuna, Cristina D'hondt, Sofie Lavaud, Johann Lepetit, Bernard Winge, Per Bones, Atle M. Vandepoele, Klaas Vyverman, Wim Sabbe, Koen 2020 application/pdf https://biblio.ugent.be/publication/8660083 http://hdl.handle.net/1854/LU-8660083 https://doi.org/10.3389/fmars.2020.00192 https://biblio.ugent.be/publication/8660083/file/8660085 eng eng https://biblio.ugent.be/publication/8660083 http://hdl.handle.net/1854/LU-8660083 http://dx.doi.org/10.3389/fmars.2020.00192 https://biblio.ugent.be/publication/8660083/file/8660085 Creative Commons Attribution 4.0 International Public License (CC-BY 4.0) info:eu-repo/semantics/openAccess FRONTIERS IN MARINE SCIENCE ISSN: 2296-7745 Biology and Life Sciences Aquatic Science Global and Planetary Change Ocean Engineering Oceanography Water Science and Technology Diatom Microphytobenthos Light Stress LHCX Physiology Phaeodactylum-tricornutum Thalassiosira-pseudonana Secondary Plastids Xanthophyll Cycle Protein Family Fluorescence State Photoprotection Expression journalArticle info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2020 ftunivgent https://doi.org/10.3389/fmars.2020.00192 2023-04-19T22:07:38Z Intertidal benthic diatoms experience a highly variable light regime, which especially challenges these organisms to cope with excess light energy during low tide. Non-photochemical quenching of chlorophyll fluorescence (NPQ) is one of the most rapid mechanisms diatoms possess to dissipate excess energy. Its capacity is mainly defined by the xanthophyll cycle (XC) and Light-Harvesting Complex X (LHCX) proteins. Whereas the XC and its relation to NPQ have been relatively well-studied in both planktonic and benthic diatoms, our current knowledge about LHCX proteins and their potential involvement in NPQ regulation is largely restricted to planktonic diatoms. While recent studies using immuno-blotting have revealed the presence of light regulated LHCX proteins in benthic diatom communities and isolates, nothing is as yet known about the diversity, identity and transcriptional regulation or function of these proteins. We identified LHCX genes in the draft genome of the model benthic diatom Seminavis robusta and followed their transcriptional regulation during a day/night cycle and during exposure to high light conditions. The S. robusta genome contains 17 LHCX sequences, which is much more than in the sequenced planktonic model diatoms (4-5), but similar to the number of LHCX genes in the sea ice associated diatom Fragilariopsis cylindrus. LHCX diversification in both species, however, appears to have occurred independently. Interestingly, the S. robusta genome contains LHCX genes that are related to the LHCX6 of the model centric diatom Thalassiosira pseudonana, which are lacking in the well-studied pennate model diatom Phaeodactylum tricornutum. All investigated LHCX genes, with exception of SrLHCX6, were upregulated during the daily dark-light transition. Exposure to 2,000 timol photons m(-2) s(-1), furthermore, increased transcription of all investigated LHCX genes. Our data suggest that the diversification and involvement of several light regulated LHCX genes in the photophysiology of S. robusta may represent ... Article in Journal/Newspaper Sea ice Ghent University Academic Bibliography Frontiers in Marine Science 7 |
institution |
Open Polar |
collection |
Ghent University Academic Bibliography |
op_collection_id |
ftunivgent |
language |
English |
topic |
Biology and Life Sciences Aquatic Science Global and Planetary Change Ocean Engineering Oceanography Water Science and Technology Diatom Microphytobenthos Light Stress LHCX Physiology Phaeodactylum-tricornutum Thalassiosira-pseudonana Secondary Plastids Xanthophyll Cycle Protein Family Fluorescence State Photoprotection Expression |
spellingShingle |
Biology and Life Sciences Aquatic Science Global and Planetary Change Ocean Engineering Oceanography Water Science and Technology Diatom Microphytobenthos Light Stress LHCX Physiology Phaeodactylum-tricornutum Thalassiosira-pseudonana Secondary Plastids Xanthophyll Cycle Protein Family Fluorescence State Photoprotection Expression Blommaert, Lander Vancaester, Emmelien Huysman, Marie Osuna, Cristina D'hondt, Sofie Lavaud, Johann Lepetit, Bernard Winge, Per Bones, Atle M. Vandepoele, Klaas Vyverman, Wim Sabbe, Koen Light regulation of LHCX genes in the benthic diatom Seminavis robusta |
topic_facet |
Biology and Life Sciences Aquatic Science Global and Planetary Change Ocean Engineering Oceanography Water Science and Technology Diatom Microphytobenthos Light Stress LHCX Physiology Phaeodactylum-tricornutum Thalassiosira-pseudonana Secondary Plastids Xanthophyll Cycle Protein Family Fluorescence State Photoprotection Expression |
description |
Intertidal benthic diatoms experience a highly variable light regime, which especially challenges these organisms to cope with excess light energy during low tide. Non-photochemical quenching of chlorophyll fluorescence (NPQ) is one of the most rapid mechanisms diatoms possess to dissipate excess energy. Its capacity is mainly defined by the xanthophyll cycle (XC) and Light-Harvesting Complex X (LHCX) proteins. Whereas the XC and its relation to NPQ have been relatively well-studied in both planktonic and benthic diatoms, our current knowledge about LHCX proteins and their potential involvement in NPQ regulation is largely restricted to planktonic diatoms. While recent studies using immuno-blotting have revealed the presence of light regulated LHCX proteins in benthic diatom communities and isolates, nothing is as yet known about the diversity, identity and transcriptional regulation or function of these proteins. We identified LHCX genes in the draft genome of the model benthic diatom Seminavis robusta and followed their transcriptional regulation during a day/night cycle and during exposure to high light conditions. The S. robusta genome contains 17 LHCX sequences, which is much more than in the sequenced planktonic model diatoms (4-5), but similar to the number of LHCX genes in the sea ice associated diatom Fragilariopsis cylindrus. LHCX diversification in both species, however, appears to have occurred independently. Interestingly, the S. robusta genome contains LHCX genes that are related to the LHCX6 of the model centric diatom Thalassiosira pseudonana, which are lacking in the well-studied pennate model diatom Phaeodactylum tricornutum. All investigated LHCX genes, with exception of SrLHCX6, were upregulated during the daily dark-light transition. Exposure to 2,000 timol photons m(-2) s(-1), furthermore, increased transcription of all investigated LHCX genes. Our data suggest that the diversification and involvement of several light regulated LHCX genes in the photophysiology of S. robusta may represent ... |
format |
Article in Journal/Newspaper |
author |
Blommaert, Lander Vancaester, Emmelien Huysman, Marie Osuna, Cristina D'hondt, Sofie Lavaud, Johann Lepetit, Bernard Winge, Per Bones, Atle M. Vandepoele, Klaas Vyverman, Wim Sabbe, Koen |
author_facet |
Blommaert, Lander Vancaester, Emmelien Huysman, Marie Osuna, Cristina D'hondt, Sofie Lavaud, Johann Lepetit, Bernard Winge, Per Bones, Atle M. Vandepoele, Klaas Vyverman, Wim Sabbe, Koen |
author_sort |
Blommaert, Lander |
title |
Light regulation of LHCX genes in the benthic diatom Seminavis robusta |
title_short |
Light regulation of LHCX genes in the benthic diatom Seminavis robusta |
title_full |
Light regulation of LHCX genes in the benthic diatom Seminavis robusta |
title_fullStr |
Light regulation of LHCX genes in the benthic diatom Seminavis robusta |
title_full_unstemmed |
Light regulation of LHCX genes in the benthic diatom Seminavis robusta |
title_sort |
light regulation of lhcx genes in the benthic diatom seminavis robusta |
publishDate |
2020 |
url |
https://biblio.ugent.be/publication/8660083 http://hdl.handle.net/1854/LU-8660083 https://doi.org/10.3389/fmars.2020.00192 https://biblio.ugent.be/publication/8660083/file/8660085 |
genre |
Sea ice |
genre_facet |
Sea ice |
op_source |
FRONTIERS IN MARINE SCIENCE ISSN: 2296-7745 |
op_relation |
https://biblio.ugent.be/publication/8660083 http://hdl.handle.net/1854/LU-8660083 http://dx.doi.org/10.3389/fmars.2020.00192 https://biblio.ugent.be/publication/8660083/file/8660085 |
op_rights |
Creative Commons Attribution 4.0 International Public License (CC-BY 4.0) info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.3389/fmars.2020.00192 |
container_title |
Frontiers in Marine Science |
container_volume |
7 |
_version_ |
1768375062348431360 |