Characterization of snakehead rhabdovirus infection in zebrafish (Danio rerio)
The zebrafish, Danio rerio, has become recognized as a valuable model for the study of development, genetics, and toxicology. Recently, the zebrafish has been recognized as a useful model for infectious disease and immunity. In this study, the pathogenesis and antiviral immune response of zebrafish...
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ftunivgent:oai:archive.ugent.be:8646046 2023-06-11T04:10:21+02:00 Characterization of snakehead rhabdovirus infection in zebrafish (Danio rerio) Phelan, P. E. Pressley, M. E. Witten, Paul Eckhard Mellon, M. T. Blake, S. Kim, C. H. 2005 application/pdf https://biblio.ugent.be/publication/8646046 http://hdl.handle.net/1854/LU-8646046 https://doi.org/10.1128/jvi.79.3.1842-1852.2005 https://biblio.ugent.be/publication/8646046/file/8646047 eng eng https://biblio.ugent.be/publication/8646046 http://hdl.handle.net/1854/LU-8646046 http://dx.doi.org/10.1128/jvi.79.3.1842-1852.2005 https://biblio.ugent.be/publication/8646046/file/8646047 No license (in copyright) info:eu-repo/semantics/restrictedAccess JOURNAL OF VIROLOGY ISSN: 0022-538X ISSN: 1098-5514 Biology and Life Sciences HUMAN MXA PROTEIN HEMATOPOIETIC NECROSIS VIRUS DOUBLE-STRANDED-RNA TOLL-LIKE RECEPTOR OPHICEPHALUS-STRIATUS CELL-LINE RAINBOW-TROUT EXPRESSION ANALYSIS INTERFERON GENE ATLANTIC SALMON journalArticle info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2005 ftunivgent https://doi.org/10.1128/jvi.79.3.1842-1852.2005 2023-05-10T22:38:27Z The zebrafish, Danio rerio, has become recognized as a valuable model for the study of development, genetics, and toxicology. Recently, the zebrafish has been recognized as a useful model for infectious disease and immunity. In this study, the pathogenesis and antiviral immune response of zebrafish to experimental snake-head rhabdovirus (SHRV) infection was characterized. Zebrafish 24 h postfertilization to 30 days postfertilization were susceptible to infection by immersion in 10(6) 50% tissue culture infective doses (TCID50) of SHRV/ml, and adult zebrafish were susceptible to infection by intraperitoneal (i.p.) injection of 10(5) TCID50 of SHRV/ml. Mortalities exceeded 40% in infected fish, and clinical presentation of infection included petechial hemorrhaging, redness of the abdomen, and erratic swim behavior. Virus reisolation and reverse transcription-PCR analysis of the viral nucleocapsid gene confirmed the presence of SHRV. Histological sections of moribund embryonic and juvenile fish revealed necrosis of the pharyngeal epithelium and liver, in addition to congestion of the swim bladder by cell debris. Histopathology in adult fish injected i.p. was confined to the site of injection. The antiviral response in zebrafish was monitored by quantitative real-time PCR analysis of zebrafish interferon (IFN) and Mx expression. IFN and Mx levels were elevated in zebrafish exposed to SHRV, although expression and intensity differed with age and route of infection. This study is the first to examine the pathogenesis of SHRV infection in zebrafish. Furthermore, this study is the first to describe experimental infection of zebrafish embryos with a viral pathogen, which will be important for future experiments involving targeted gene disruption and forward genetic screens. Article in Journal/Newspaper Atlantic salmon Ghent University Academic Bibliography Journal of Virology 79 3 1842 1852 |
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Open Polar |
collection |
Ghent University Academic Bibliography |
op_collection_id |
ftunivgent |
language |
English |
topic |
Biology and Life Sciences HUMAN MXA PROTEIN HEMATOPOIETIC NECROSIS VIRUS DOUBLE-STRANDED-RNA TOLL-LIKE RECEPTOR OPHICEPHALUS-STRIATUS CELL-LINE RAINBOW-TROUT EXPRESSION ANALYSIS INTERFERON GENE ATLANTIC SALMON |
spellingShingle |
Biology and Life Sciences HUMAN MXA PROTEIN HEMATOPOIETIC NECROSIS VIRUS DOUBLE-STRANDED-RNA TOLL-LIKE RECEPTOR OPHICEPHALUS-STRIATUS CELL-LINE RAINBOW-TROUT EXPRESSION ANALYSIS INTERFERON GENE ATLANTIC SALMON Phelan, P. E. Pressley, M. E. Witten, Paul Eckhard Mellon, M. T. Blake, S. Kim, C. H. Characterization of snakehead rhabdovirus infection in zebrafish (Danio rerio) |
topic_facet |
Biology and Life Sciences HUMAN MXA PROTEIN HEMATOPOIETIC NECROSIS VIRUS DOUBLE-STRANDED-RNA TOLL-LIKE RECEPTOR OPHICEPHALUS-STRIATUS CELL-LINE RAINBOW-TROUT EXPRESSION ANALYSIS INTERFERON GENE ATLANTIC SALMON |
description |
The zebrafish, Danio rerio, has become recognized as a valuable model for the study of development, genetics, and toxicology. Recently, the zebrafish has been recognized as a useful model for infectious disease and immunity. In this study, the pathogenesis and antiviral immune response of zebrafish to experimental snake-head rhabdovirus (SHRV) infection was characterized. Zebrafish 24 h postfertilization to 30 days postfertilization were susceptible to infection by immersion in 10(6) 50% tissue culture infective doses (TCID50) of SHRV/ml, and adult zebrafish were susceptible to infection by intraperitoneal (i.p.) injection of 10(5) TCID50 of SHRV/ml. Mortalities exceeded 40% in infected fish, and clinical presentation of infection included petechial hemorrhaging, redness of the abdomen, and erratic swim behavior. Virus reisolation and reverse transcription-PCR analysis of the viral nucleocapsid gene confirmed the presence of SHRV. Histological sections of moribund embryonic and juvenile fish revealed necrosis of the pharyngeal epithelium and liver, in addition to congestion of the swim bladder by cell debris. Histopathology in adult fish injected i.p. was confined to the site of injection. The antiviral response in zebrafish was monitored by quantitative real-time PCR analysis of zebrafish interferon (IFN) and Mx expression. IFN and Mx levels were elevated in zebrafish exposed to SHRV, although expression and intensity differed with age and route of infection. This study is the first to examine the pathogenesis of SHRV infection in zebrafish. Furthermore, this study is the first to describe experimental infection of zebrafish embryos with a viral pathogen, which will be important for future experiments involving targeted gene disruption and forward genetic screens. |
format |
Article in Journal/Newspaper |
author |
Phelan, P. E. Pressley, M. E. Witten, Paul Eckhard Mellon, M. T. Blake, S. Kim, C. H. |
author_facet |
Phelan, P. E. Pressley, M. E. Witten, Paul Eckhard Mellon, M. T. Blake, S. Kim, C. H. |
author_sort |
Phelan, P. E. |
title |
Characterization of snakehead rhabdovirus infection in zebrafish (Danio rerio) |
title_short |
Characterization of snakehead rhabdovirus infection in zebrafish (Danio rerio) |
title_full |
Characterization of snakehead rhabdovirus infection in zebrafish (Danio rerio) |
title_fullStr |
Characterization of snakehead rhabdovirus infection in zebrafish (Danio rerio) |
title_full_unstemmed |
Characterization of snakehead rhabdovirus infection in zebrafish (Danio rerio) |
title_sort |
characterization of snakehead rhabdovirus infection in zebrafish (danio rerio) |
publishDate |
2005 |
url |
https://biblio.ugent.be/publication/8646046 http://hdl.handle.net/1854/LU-8646046 https://doi.org/10.1128/jvi.79.3.1842-1852.2005 https://biblio.ugent.be/publication/8646046/file/8646047 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
JOURNAL OF VIROLOGY ISSN: 0022-538X ISSN: 1098-5514 |
op_relation |
https://biblio.ugent.be/publication/8646046 http://hdl.handle.net/1854/LU-8646046 http://dx.doi.org/10.1128/jvi.79.3.1842-1852.2005 https://biblio.ugent.be/publication/8646046/file/8646047 |
op_rights |
No license (in copyright) info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1128/jvi.79.3.1842-1852.2005 |
container_title |
Journal of Virology |
container_volume |
79 |
container_issue |
3 |
container_start_page |
1842 |
op_container_end_page |
1852 |
_version_ |
1768384699020869632 |