Pinpointing the role of Aeromonas salmonicida in the development of skin ulcerations in common dab (Limanda limanda)
Aeromonas salmonicida was isolated from ulcerations in common dab (Limanda limanda). An experiment was performed to pinpoint its role in ulceration development, considering the importance of the skin barrier and the pigmented and non-pigmented sides. The skin of dab was treated in three zones, one w...
Published in: | Journal of Fish Diseases |
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2020
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Online Access: | https://biblio.ugent.be/publication/8644008 http://hdl.handle.net/1854/LU-8644008 https://doi.org/10.1111/jfd.13133 https://biblio.ugent.be/publication/8644008/file/8656050 |
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ftunivgent:oai:archive.ugent.be:8644008 2023-06-11T04:10:20+02:00 Pinpointing the role of Aeromonas salmonicida in the development of skin ulcerations in common dab (Limanda limanda) Vercauteren, Maaike De Swaef, Evelien Declercq, Annelies Aerts, Johan Ampe, Bart Gulla, Snorre Haesebrouck, Freddy Devriese, Lisa Decostere, Annemie Chiers, Koen 2020 application/pdf https://biblio.ugent.be/publication/8644008 http://hdl.handle.net/1854/LU-8644008 https://doi.org/10.1111/jfd.13133 https://biblio.ugent.be/publication/8644008/file/8656050 eng eng https://biblio.ugent.be/publication/8644008 http://hdl.handle.net/1854/LU-8644008 http://dx.doi.org/10.1111/jfd.13133 https://biblio.ugent.be/publication/8644008/file/8656050 No license (in copyright) info:eu-repo/semantics/restrictedAccess JOURNAL OF FISH DISEASES ISSN: 0140-7775 ISSN: 1365-2761 Biology and Life Sciences Veterinary Sciences Aeromonas salmonicida A-layer typing common dab skin barrier skin ulceration FLOUNDER PLATICHTHYS-FLESUS EPIDERMAL PAPILLOMA NUCLEOTIDE-SEQUENCE ATLANTIC SALMON ULCER DISEASE BALTIC SEA FISH MORTALITY TURBOT INFECTION journalArticle info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2020 ftunivgent https://doi.org/10.1111/jfd.13133 2023-05-10T22:53:30Z Aeromonas salmonicida was isolated from ulcerations in common dab (Limanda limanda). An experiment was performed to pinpoint its role in ulceration development, considering the importance of the skin barrier and the pigmented and non-pigmented sides. The skin of dab was treated in three zones, one where scales and epidermis were removed, one where mucus was discarded and one non-treated zone. Fish were tagged to allow individual identification and challenged with A. salmonicida. Mortality and severity of the developing lesions were recorded for 21 days post-inoculation. Starting 12 days post-inoculation, mortality occurred gradually in challenged fish; however, no direct cause could be established. Both control fish and challenged fish developed ulcerations containing A. salmonicida. Sequencing of vapA gene revealed that isolates retrieved from both groups were distinct, suggesting the presence of A. salmonicida prior to the trial. Most ulcerations developed in zones where skin was removed, suggesting that abrasion might be a predisposing factor in ulceration development. Ulcerations were also observed at the insertion site of the tag, where exposed muscle tissue might have favoured the development of ulcerations. In conclusion, A. salmonicida seems to be involved in the development of skin ulcerations in dab, although the exact pathogenesis needs to be elucidated. Article in Journal/Newspaper Atlantic salmon Turbot Ghent University Academic Bibliography Journal of Fish Diseases 43 3 347 357 |
institution |
Open Polar |
collection |
Ghent University Academic Bibliography |
op_collection_id |
ftunivgent |
language |
English |
topic |
Biology and Life Sciences Veterinary Sciences Aeromonas salmonicida A-layer typing common dab skin barrier skin ulceration FLOUNDER PLATICHTHYS-FLESUS EPIDERMAL PAPILLOMA NUCLEOTIDE-SEQUENCE ATLANTIC SALMON ULCER DISEASE BALTIC SEA FISH MORTALITY TURBOT INFECTION |
spellingShingle |
Biology and Life Sciences Veterinary Sciences Aeromonas salmonicida A-layer typing common dab skin barrier skin ulceration FLOUNDER PLATICHTHYS-FLESUS EPIDERMAL PAPILLOMA NUCLEOTIDE-SEQUENCE ATLANTIC SALMON ULCER DISEASE BALTIC SEA FISH MORTALITY TURBOT INFECTION Vercauteren, Maaike De Swaef, Evelien Declercq, Annelies Aerts, Johan Ampe, Bart Gulla, Snorre Haesebrouck, Freddy Devriese, Lisa Decostere, Annemie Chiers, Koen Pinpointing the role of Aeromonas salmonicida in the development of skin ulcerations in common dab (Limanda limanda) |
topic_facet |
Biology and Life Sciences Veterinary Sciences Aeromonas salmonicida A-layer typing common dab skin barrier skin ulceration FLOUNDER PLATICHTHYS-FLESUS EPIDERMAL PAPILLOMA NUCLEOTIDE-SEQUENCE ATLANTIC SALMON ULCER DISEASE BALTIC SEA FISH MORTALITY TURBOT INFECTION |
description |
Aeromonas salmonicida was isolated from ulcerations in common dab (Limanda limanda). An experiment was performed to pinpoint its role in ulceration development, considering the importance of the skin barrier and the pigmented and non-pigmented sides. The skin of dab was treated in three zones, one where scales and epidermis were removed, one where mucus was discarded and one non-treated zone. Fish were tagged to allow individual identification and challenged with A. salmonicida. Mortality and severity of the developing lesions were recorded for 21 days post-inoculation. Starting 12 days post-inoculation, mortality occurred gradually in challenged fish; however, no direct cause could be established. Both control fish and challenged fish developed ulcerations containing A. salmonicida. Sequencing of vapA gene revealed that isolates retrieved from both groups were distinct, suggesting the presence of A. salmonicida prior to the trial. Most ulcerations developed in zones where skin was removed, suggesting that abrasion might be a predisposing factor in ulceration development. Ulcerations were also observed at the insertion site of the tag, where exposed muscle tissue might have favoured the development of ulcerations. In conclusion, A. salmonicida seems to be involved in the development of skin ulcerations in dab, although the exact pathogenesis needs to be elucidated. |
format |
Article in Journal/Newspaper |
author |
Vercauteren, Maaike De Swaef, Evelien Declercq, Annelies Aerts, Johan Ampe, Bart Gulla, Snorre Haesebrouck, Freddy Devriese, Lisa Decostere, Annemie Chiers, Koen |
author_facet |
Vercauteren, Maaike De Swaef, Evelien Declercq, Annelies Aerts, Johan Ampe, Bart Gulla, Snorre Haesebrouck, Freddy Devriese, Lisa Decostere, Annemie Chiers, Koen |
author_sort |
Vercauteren, Maaike |
title |
Pinpointing the role of Aeromonas salmonicida in the development of skin ulcerations in common dab (Limanda limanda) |
title_short |
Pinpointing the role of Aeromonas salmonicida in the development of skin ulcerations in common dab (Limanda limanda) |
title_full |
Pinpointing the role of Aeromonas salmonicida in the development of skin ulcerations in common dab (Limanda limanda) |
title_fullStr |
Pinpointing the role of Aeromonas salmonicida in the development of skin ulcerations in common dab (Limanda limanda) |
title_full_unstemmed |
Pinpointing the role of Aeromonas salmonicida in the development of skin ulcerations in common dab (Limanda limanda) |
title_sort |
pinpointing the role of aeromonas salmonicida in the development of skin ulcerations in common dab (limanda limanda) |
publishDate |
2020 |
url |
https://biblio.ugent.be/publication/8644008 http://hdl.handle.net/1854/LU-8644008 https://doi.org/10.1111/jfd.13133 https://biblio.ugent.be/publication/8644008/file/8656050 |
genre |
Atlantic salmon Turbot |
genre_facet |
Atlantic salmon Turbot |
op_source |
JOURNAL OF FISH DISEASES ISSN: 0140-7775 ISSN: 1365-2761 |
op_relation |
https://biblio.ugent.be/publication/8644008 http://hdl.handle.net/1854/LU-8644008 http://dx.doi.org/10.1111/jfd.13133 https://biblio.ugent.be/publication/8644008/file/8656050 |
op_rights |
No license (in copyright) info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1111/jfd.13133 |
container_title |
Journal of Fish Diseases |
container_volume |
43 |
container_issue |
3 |
container_start_page |
347 |
op_container_end_page |
357 |
_version_ |
1768384684875579392 |