Get salty with me : acetate production through homoacetogenesis in (hyper)saline conditions
Homoacetogenesis has become a hot topic, with industry interest, as a bio-process that can convert waste CO2 directly to a valorizable product (organic acids). However, the microorganisms catalyzing this reaction have remained little explored outside of their original discovery and later in the pion...
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ftunivgent:oai:archive.ugent.be:8643183 2024-02-11T10:06:48+01:00 Get salty with me : acetate production through homoacetogenesis in (hyper)saline conditions Arbour, Tyler 2019 https://biblio.ugent.be/publication/8643183 http://hdl.handle.net/1854/LU-8643183 eng eng https://biblio.ugent.be/publication/8643183 http://hdl.handle.net/1854/LU-8643183 ISMET 7, International Society for Microbial Electrochemistry and Technology, 7th Global Conference, Abstracts Biology and Life Sciences acetate acetogenesis saline north sea sediment anaerobic bioproduction conference info:eu-repo/semantics/conferenceObject info:eu-repo/semantics/publishedVersion 2019 ftunivgent 2024-01-24T23:09:01Z Homoacetogenesis has become a hot topic, with industry interest, as a bio-process that can convert waste CO2 directly to a valorizable product (organic acids). However, the microorganisms catalyzing this reaction have remained little explored outside of their original discovery and later in the pioneering work on a few isolates in elucidation of the Wood-Ljungdahl pathway. We are exploring homoacetogenesis in saline environments for two main reasons: i) application in microbial electrosynthesis (MES) reactors under saline conditions, where salinity decreases ohmic resistance and inhibits competing methanogens by salt stress; and ii) fundamental microbiological interest. Motivating questions include: Can we find and enrich for novel salt-tolerant homoacetogens from un- or underexplored environments? Where is homoacetogenesis most active in the depth/redox profile of saline soil and sediment? We collected samples from North Atlantic coastal marine sediment (10 m.b.s.l.) and from high-altitude (3921 m.a.s.l.) shallow salt lakes of the Argentinian Andes. Serum-flask enrichments of North Sea samples in a homoacetogen seawater medium (35 g/L salinity) using a headspace of H2/CO2 (80/20 vol%) showed an initial period of acetate production (1-2 g/L in two weeks) from the top 10 cm of sediment, followed by a switch to methanogenesis. Samples collected from a salt lake in Argentina ranged in salinity from ~10 to 90 g/L total salts. We therefore explored the correlation between environmental salinity and acetate-production capacity in culture media of 20, 60, and 100 g/L salts, also with a H2/CO2 headspace. Acetate concentrations in the 20-, 60-, and 100 g/L media reached 8500, 3000, and 73 mg/L, respectively, after 150 days. Furthermore, methane production was only observed in one of the 20 g/L incubations. Results from 16S rRNA amplicon sequencing, targeted RNA-based qPCR and flow cytometry of environmental samples and enriched cultures will also be presented. Finally, we have begun testing the performance of selected ... Conference Object North Atlantic Ghent University Academic Bibliography Argentina |
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Open Polar |
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Ghent University Academic Bibliography |
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ftunivgent |
language |
English |
topic |
Biology and Life Sciences acetate acetogenesis saline north sea sediment anaerobic bioproduction |
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Biology and Life Sciences acetate acetogenesis saline north sea sediment anaerobic bioproduction Arbour, Tyler Get salty with me : acetate production through homoacetogenesis in (hyper)saline conditions |
topic_facet |
Biology and Life Sciences acetate acetogenesis saline north sea sediment anaerobic bioproduction |
description |
Homoacetogenesis has become a hot topic, with industry interest, as a bio-process that can convert waste CO2 directly to a valorizable product (organic acids). However, the microorganisms catalyzing this reaction have remained little explored outside of their original discovery and later in the pioneering work on a few isolates in elucidation of the Wood-Ljungdahl pathway. We are exploring homoacetogenesis in saline environments for two main reasons: i) application in microbial electrosynthesis (MES) reactors under saline conditions, where salinity decreases ohmic resistance and inhibits competing methanogens by salt stress; and ii) fundamental microbiological interest. Motivating questions include: Can we find and enrich for novel salt-tolerant homoacetogens from un- or underexplored environments? Where is homoacetogenesis most active in the depth/redox profile of saline soil and sediment? We collected samples from North Atlantic coastal marine sediment (10 m.b.s.l.) and from high-altitude (3921 m.a.s.l.) shallow salt lakes of the Argentinian Andes. Serum-flask enrichments of North Sea samples in a homoacetogen seawater medium (35 g/L salinity) using a headspace of H2/CO2 (80/20 vol%) showed an initial period of acetate production (1-2 g/L in two weeks) from the top 10 cm of sediment, followed by a switch to methanogenesis. Samples collected from a salt lake in Argentina ranged in salinity from ~10 to 90 g/L total salts. We therefore explored the correlation between environmental salinity and acetate-production capacity in culture media of 20, 60, and 100 g/L salts, also with a H2/CO2 headspace. Acetate concentrations in the 20-, 60-, and 100 g/L media reached 8500, 3000, and 73 mg/L, respectively, after 150 days. Furthermore, methane production was only observed in one of the 20 g/L incubations. Results from 16S rRNA amplicon sequencing, targeted RNA-based qPCR and flow cytometry of environmental samples and enriched cultures will also be presented. Finally, we have begun testing the performance of selected ... |
format |
Conference Object |
author |
Arbour, Tyler |
author_facet |
Arbour, Tyler |
author_sort |
Arbour, Tyler |
title |
Get salty with me : acetate production through homoacetogenesis in (hyper)saline conditions |
title_short |
Get salty with me : acetate production through homoacetogenesis in (hyper)saline conditions |
title_full |
Get salty with me : acetate production through homoacetogenesis in (hyper)saline conditions |
title_fullStr |
Get salty with me : acetate production through homoacetogenesis in (hyper)saline conditions |
title_full_unstemmed |
Get salty with me : acetate production through homoacetogenesis in (hyper)saline conditions |
title_sort |
get salty with me : acetate production through homoacetogenesis in (hyper)saline conditions |
publishDate |
2019 |
url |
https://biblio.ugent.be/publication/8643183 http://hdl.handle.net/1854/LU-8643183 |
geographic |
Argentina |
geographic_facet |
Argentina |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_source |
ISMET 7, International Society for Microbial Electrochemistry and Technology, 7th Global Conference, Abstracts |
op_relation |
https://biblio.ugent.be/publication/8643183 http://hdl.handle.net/1854/LU-8643183 |
_version_ |
1790604769699561472 |