Assay optimisation and age-related baseline variation in biochemical markers in lesser black-backed gulls
Free-ranging animals are often used as bioindicators of both short- and long-term changes in ecosystem health, mainly to detect the presence and effects of contaminants. Birds, and gulls in particular, have been used as bioindicators over a broad range of marine and terrestrial ecosystems. In this s...
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ftunivgent:oai:archive.ugent.be:8612671 2023-06-11T04:13:50+02:00 Assay optimisation and age-related baseline variation in biochemical markers in lesser black-backed gulls Andrade Dos Santos, Cátia Sofia Loureiro, Susana Sotillo, Alejandro Mueller, Wendt Stienen, Eric WM De Neve, Liesbeth Lens, Luc Monteiro, Marta S 2019 application/pdf https://biblio.ugent.be/publication/8612671 http://hdl.handle.net/1854/LU-8612671 https://doi.org/10.1016/j.ecoenv.2019.01.084 https://biblio.ugent.be/publication/8612671/file/8612672 eng eng https://biblio.ugent.be/publication/8612671 http://hdl.handle.net/1854/LU-8612671 http://dx.doi.org/10.1016/j.ecoenv.2019.01.084 https://biblio.ugent.be/publication/8612671/file/8612672 No license (in copyright) info:eu-repo/semantics/restrictedAccess ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY ISSN: 0147-6513 ISSN: 1090-2414 Biology and Life Sciences OXIDATIVE STRESS PESTICIDE EXPOSURE CHOLINESTERASE BIOMARKERS PLASMA NESTLINGS INDICATOR BLOOD CONTAMINATION SELECTION Lactate dehydrogenase Oxidative stress Gulls journalArticle info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2019 ftunivgent https://doi.org/10.1016/j.ecoenv.2019.01.084 2023-05-10T22:23:33Z Free-ranging animals are often used as bioindicators of both short- and long-term changes in ecosystem health, mainly to detect the presence and effects of contaminants. Birds, and gulls in particular, have been used as bioindicators over a broad range of marine and terrestrial ecosystems. In this study, we standardise the conditions for the use of a suite of biochemical markers in non-destructive matrices of Lesser Black-backed Gull (Larus fuscus) to facilitate future biomonitoring of marine and terrestrial contaminants. We characterized cholinesterase (ChE) in plasma and optimized assay conditions for ChE activity as a marker of neurotoxic damage. Moreover, we quantified variation in activity of ChE, lactate dehydrogenase (LDH), glutathione-S-transferase (GST) and catalase (CAT) as well as variation ranges of lipid peroxidation (LPO), in free-ranging adults and captive chicks. The main ChE form present in plasma of both adults and chicks was butyrylcholinesterase (BChE) followed by acetylcholinesterase (AChE), whose relative proportion in plasma tended to decrease with increased chick age. LPO levels and GST activity in blood cells (BCs) decreased significantly with increasing chick age, while BChE and LDH activity in plasma were not age-dependent. CAT in BCs tended to decline non-significantly in older chicks. Results of this study underscore the importance of standardising assay conditions and assessing intrinsic baseline variation in biochemical markers, before biochemical quantification. Data presented here provide a foundation for future use of BChE and LDH activity in plasma, as well as oxidative stress markers (LPO, CAT and GST) in BCs, to monitor environmental stress effects in Lesser Black-backed gulls. Article in Journal/Newspaper Lesser black-backed gull Ghent University Academic Bibliography Ecotoxicology and Environmental Safety 172 246 254 |
institution |
Open Polar |
collection |
Ghent University Academic Bibliography |
op_collection_id |
ftunivgent |
language |
English |
topic |
Biology and Life Sciences OXIDATIVE STRESS PESTICIDE EXPOSURE CHOLINESTERASE BIOMARKERS PLASMA NESTLINGS INDICATOR BLOOD CONTAMINATION SELECTION Lactate dehydrogenase Oxidative stress Gulls |
spellingShingle |
Biology and Life Sciences OXIDATIVE STRESS PESTICIDE EXPOSURE CHOLINESTERASE BIOMARKERS PLASMA NESTLINGS INDICATOR BLOOD CONTAMINATION SELECTION Lactate dehydrogenase Oxidative stress Gulls Andrade Dos Santos, Cátia Sofia Loureiro, Susana Sotillo, Alejandro Mueller, Wendt Stienen, Eric WM De Neve, Liesbeth Lens, Luc Monteiro, Marta S Assay optimisation and age-related baseline variation in biochemical markers in lesser black-backed gulls |
topic_facet |
Biology and Life Sciences OXIDATIVE STRESS PESTICIDE EXPOSURE CHOLINESTERASE BIOMARKERS PLASMA NESTLINGS INDICATOR BLOOD CONTAMINATION SELECTION Lactate dehydrogenase Oxidative stress Gulls |
description |
Free-ranging animals are often used as bioindicators of both short- and long-term changes in ecosystem health, mainly to detect the presence and effects of contaminants. Birds, and gulls in particular, have been used as bioindicators over a broad range of marine and terrestrial ecosystems. In this study, we standardise the conditions for the use of a suite of biochemical markers in non-destructive matrices of Lesser Black-backed Gull (Larus fuscus) to facilitate future biomonitoring of marine and terrestrial contaminants. We characterized cholinesterase (ChE) in plasma and optimized assay conditions for ChE activity as a marker of neurotoxic damage. Moreover, we quantified variation in activity of ChE, lactate dehydrogenase (LDH), glutathione-S-transferase (GST) and catalase (CAT) as well as variation ranges of lipid peroxidation (LPO), in free-ranging adults and captive chicks. The main ChE form present in plasma of both adults and chicks was butyrylcholinesterase (BChE) followed by acetylcholinesterase (AChE), whose relative proportion in plasma tended to decrease with increased chick age. LPO levels and GST activity in blood cells (BCs) decreased significantly with increasing chick age, while BChE and LDH activity in plasma were not age-dependent. CAT in BCs tended to decline non-significantly in older chicks. Results of this study underscore the importance of standardising assay conditions and assessing intrinsic baseline variation in biochemical markers, before biochemical quantification. Data presented here provide a foundation for future use of BChE and LDH activity in plasma, as well as oxidative stress markers (LPO, CAT and GST) in BCs, to monitor environmental stress effects in Lesser Black-backed gulls. |
format |
Article in Journal/Newspaper |
author |
Andrade Dos Santos, Cátia Sofia Loureiro, Susana Sotillo, Alejandro Mueller, Wendt Stienen, Eric WM De Neve, Liesbeth Lens, Luc Monteiro, Marta S |
author_facet |
Andrade Dos Santos, Cátia Sofia Loureiro, Susana Sotillo, Alejandro Mueller, Wendt Stienen, Eric WM De Neve, Liesbeth Lens, Luc Monteiro, Marta S |
author_sort |
Andrade Dos Santos, Cátia Sofia |
title |
Assay optimisation and age-related baseline variation in biochemical markers in lesser black-backed gulls |
title_short |
Assay optimisation and age-related baseline variation in biochemical markers in lesser black-backed gulls |
title_full |
Assay optimisation and age-related baseline variation in biochemical markers in lesser black-backed gulls |
title_fullStr |
Assay optimisation and age-related baseline variation in biochemical markers in lesser black-backed gulls |
title_full_unstemmed |
Assay optimisation and age-related baseline variation in biochemical markers in lesser black-backed gulls |
title_sort |
assay optimisation and age-related baseline variation in biochemical markers in lesser black-backed gulls |
publishDate |
2019 |
url |
https://biblio.ugent.be/publication/8612671 http://hdl.handle.net/1854/LU-8612671 https://doi.org/10.1016/j.ecoenv.2019.01.084 https://biblio.ugent.be/publication/8612671/file/8612672 |
genre |
Lesser black-backed gull |
genre_facet |
Lesser black-backed gull |
op_source |
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY ISSN: 0147-6513 ISSN: 1090-2414 |
op_relation |
https://biblio.ugent.be/publication/8612671 http://hdl.handle.net/1854/LU-8612671 http://dx.doi.org/10.1016/j.ecoenv.2019.01.084 https://biblio.ugent.be/publication/8612671/file/8612672 |
op_rights |
No license (in copyright) info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1016/j.ecoenv.2019.01.084 |
container_title |
Ecotoxicology and Environmental Safety |
container_volume |
172 |
container_start_page |
246 |
op_container_end_page |
254 |
_version_ |
1768391222971334656 |