Diversity of key genes for carbon and nitrogen fixation in soils from the Sør Rondane Mountains, East Antarctica
Although Cyanobacteria are generally considered the most important primary producers and diazotrophs in Antarctic terrestrial ecosystems, in several high-altitude Antarctic regions, they were previously reported to be relatively rare in some samples. In view of these observations, we investigated th...
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ftunivgent:oai:archive.ugent.be:8584251 2023-06-11T04:06:01+02:00 Diversity of key genes for carbon and nitrogen fixation in soils from the Sør Rondane Mountains, East Antarctica Tahon, Guillaume Tytgat, Bjorn Willems, Anne 2018 application/pdf https://biblio.ugent.be/publication/8584251 http://hdl.handle.net/1854/LU-8584251 https://doi.org/10.1007/s00300-018-2353-y https://biblio.ugent.be/publication/8584251/file/8584259 eng eng https://biblio.ugent.be/publication/8584251 http://hdl.handle.net/1854/LU-8584251 http://dx.doi.org/10.1007/s00300-018-2353-y https://biblio.ugent.be/publication/8584251/file/8584259 No license (in copyright) info:eu-repo/semantics/restrictedAccess POLAR BIOLOGY ISSN: 0722-4060 ISSN: 1432-2056 Biology and Life Sciences Earth and Environmental Sciences RIBULOSE 1 5-BISPHOSPHATE CARBOXYLASE/OXYGENASE RIBOSOMAL-RNA SEQUENCES LARGE-SUBUNIT GENES CYANOBACTERIAL DIVERSITY AUTOTROPHIC BACTERIA CO2 FIXATION DRY VALLEYS CD-HIT CYCLE BIODIVERSITY RuBisCO Diazotrophy Sequencing Autotrophy Community analysis Antarctic journalArticle info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2018 ftunivgent https://doi.org/10.1007/s00300-018-2353-y 2023-05-10T22:52:32Z Although Cyanobacteria are generally considered the most important primary producers and diazotrophs in Antarctic terrestrial ecosystems, in several high-altitude Antarctic regions, they were previously reported to be relatively rare in some samples. In view of these observations, we investigated the presence of non-cyanobacterial carbon- and nitrogen-fixing microorganisms in oligotrophic exposed soils without visible cyanobacterial biomass from the Sor Rondane Mountains, East Antarctica. An Illumina amplicon sequencing approach was used to analyze the bacterial community composition and the diversity and abundance of key genes involved in the carbon- and nitrogen-fixation processes. Analysis of the large subunit of type I ribulose-1,5-biphosphate carboxylase/oxygenase genes (cbbL) revealed a large actinobacterial, and alpha-, beta-, and gammaproteobacterial diversity of CbbL type IC, whereas type IA diversity was restricted to Bradyrhizobium sp.-like sequences. Although a large portion of the CbbL sequences grouped with those of cultivated bacteria, some belonged to currently unknown phylotypes. Data of 16S rRNA genes, however, also revealed that samples contained either considerable numbers of Cyanobacteria or Trebouxiophyceae as oxygenic phototrophic primary producers. Dinitrogenase-reductase genes (nifH) most similar to those of Nostocales cyanobacteria were dominantly retrieved from these oligotrophic soils. These findings suggest that diverse microorganisms capable of assimilating carbon dioxide through the Calvin-Benson-Bassham cycle inhabit these extreme terrestrial systems and potentially may contribute to primary production. However, Cyanobacteria, present in greatly varying numbers as assessed by Illumina amplicon sequencing of a 16S rRNA gene fragment, appear to be the most important nitrogen fixers in these habitats. Article in Journal/Newspaper Antarc* Antarctic Antarctica East Antarctica Polar Biology Ghent University Academic Bibliography Antarctic Calvin ENVELOPE(165.100,165.100,-71.283,-71.283) East Antarctica Sor Rondane Mountains ENVELOPE(25.000,25.000,-72.000,-72.000) Sor-Rondane ENVELOPE(25.000,25.000,-72.000,-72.000) Sør Rondane Mountains ENVELOPE(25.000,25.000,-72.000,-72.000) Sør-Rondane ENVELOPE(25.000,25.000,-72.000,-72.000) Polar Biology 41 11 2181 2198 |
institution |
Open Polar |
collection |
Ghent University Academic Bibliography |
op_collection_id |
ftunivgent |
language |
English |
topic |
Biology and Life Sciences Earth and Environmental Sciences RIBULOSE 1 5-BISPHOSPHATE CARBOXYLASE/OXYGENASE RIBOSOMAL-RNA SEQUENCES LARGE-SUBUNIT GENES CYANOBACTERIAL DIVERSITY AUTOTROPHIC BACTERIA CO2 FIXATION DRY VALLEYS CD-HIT CYCLE BIODIVERSITY RuBisCO Diazotrophy Sequencing Autotrophy Community analysis Antarctic |
spellingShingle |
Biology and Life Sciences Earth and Environmental Sciences RIBULOSE 1 5-BISPHOSPHATE CARBOXYLASE/OXYGENASE RIBOSOMAL-RNA SEQUENCES LARGE-SUBUNIT GENES CYANOBACTERIAL DIVERSITY AUTOTROPHIC BACTERIA CO2 FIXATION DRY VALLEYS CD-HIT CYCLE BIODIVERSITY RuBisCO Diazotrophy Sequencing Autotrophy Community analysis Antarctic Tahon, Guillaume Tytgat, Bjorn Willems, Anne Diversity of key genes for carbon and nitrogen fixation in soils from the Sør Rondane Mountains, East Antarctica |
topic_facet |
Biology and Life Sciences Earth and Environmental Sciences RIBULOSE 1 5-BISPHOSPHATE CARBOXYLASE/OXYGENASE RIBOSOMAL-RNA SEQUENCES LARGE-SUBUNIT GENES CYANOBACTERIAL DIVERSITY AUTOTROPHIC BACTERIA CO2 FIXATION DRY VALLEYS CD-HIT CYCLE BIODIVERSITY RuBisCO Diazotrophy Sequencing Autotrophy Community analysis Antarctic |
description |
Although Cyanobacteria are generally considered the most important primary producers and diazotrophs in Antarctic terrestrial ecosystems, in several high-altitude Antarctic regions, they were previously reported to be relatively rare in some samples. In view of these observations, we investigated the presence of non-cyanobacterial carbon- and nitrogen-fixing microorganisms in oligotrophic exposed soils without visible cyanobacterial biomass from the Sor Rondane Mountains, East Antarctica. An Illumina amplicon sequencing approach was used to analyze the bacterial community composition and the diversity and abundance of key genes involved in the carbon- and nitrogen-fixation processes. Analysis of the large subunit of type I ribulose-1,5-biphosphate carboxylase/oxygenase genes (cbbL) revealed a large actinobacterial, and alpha-, beta-, and gammaproteobacterial diversity of CbbL type IC, whereas type IA diversity was restricted to Bradyrhizobium sp.-like sequences. Although a large portion of the CbbL sequences grouped with those of cultivated bacteria, some belonged to currently unknown phylotypes. Data of 16S rRNA genes, however, also revealed that samples contained either considerable numbers of Cyanobacteria or Trebouxiophyceae as oxygenic phototrophic primary producers. Dinitrogenase-reductase genes (nifH) most similar to those of Nostocales cyanobacteria were dominantly retrieved from these oligotrophic soils. These findings suggest that diverse microorganisms capable of assimilating carbon dioxide through the Calvin-Benson-Bassham cycle inhabit these extreme terrestrial systems and potentially may contribute to primary production. However, Cyanobacteria, present in greatly varying numbers as assessed by Illumina amplicon sequencing of a 16S rRNA gene fragment, appear to be the most important nitrogen fixers in these habitats. |
format |
Article in Journal/Newspaper |
author |
Tahon, Guillaume Tytgat, Bjorn Willems, Anne |
author_facet |
Tahon, Guillaume Tytgat, Bjorn Willems, Anne |
author_sort |
Tahon, Guillaume |
title |
Diversity of key genes for carbon and nitrogen fixation in soils from the Sør Rondane Mountains, East Antarctica |
title_short |
Diversity of key genes for carbon and nitrogen fixation in soils from the Sør Rondane Mountains, East Antarctica |
title_full |
Diversity of key genes for carbon and nitrogen fixation in soils from the Sør Rondane Mountains, East Antarctica |
title_fullStr |
Diversity of key genes for carbon and nitrogen fixation in soils from the Sør Rondane Mountains, East Antarctica |
title_full_unstemmed |
Diversity of key genes for carbon and nitrogen fixation in soils from the Sør Rondane Mountains, East Antarctica |
title_sort |
diversity of key genes for carbon and nitrogen fixation in soils from the sør rondane mountains, east antarctica |
publishDate |
2018 |
url |
https://biblio.ugent.be/publication/8584251 http://hdl.handle.net/1854/LU-8584251 https://doi.org/10.1007/s00300-018-2353-y https://biblio.ugent.be/publication/8584251/file/8584259 |
long_lat |
ENVELOPE(165.100,165.100,-71.283,-71.283) ENVELOPE(25.000,25.000,-72.000,-72.000) ENVELOPE(25.000,25.000,-72.000,-72.000) ENVELOPE(25.000,25.000,-72.000,-72.000) ENVELOPE(25.000,25.000,-72.000,-72.000) |
geographic |
Antarctic Calvin East Antarctica Sor Rondane Mountains Sor-Rondane Sør Rondane Mountains Sør-Rondane |
geographic_facet |
Antarctic Calvin East Antarctica Sor Rondane Mountains Sor-Rondane Sør Rondane Mountains Sør-Rondane |
genre |
Antarc* Antarctic Antarctica East Antarctica Polar Biology |
genre_facet |
Antarc* Antarctic Antarctica East Antarctica Polar Biology |
op_source |
POLAR BIOLOGY ISSN: 0722-4060 ISSN: 1432-2056 |
op_relation |
https://biblio.ugent.be/publication/8584251 http://hdl.handle.net/1854/LU-8584251 http://dx.doi.org/10.1007/s00300-018-2353-y https://biblio.ugent.be/publication/8584251/file/8584259 |
op_rights |
No license (in copyright) info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1007/s00300-018-2353-y |
container_title |
Polar Biology |
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41 |
container_issue |
11 |
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2181 |
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2198 |
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1768377750987472896 |