Single tracheal inoculation of Aspergillus fumigatus conidia induced aspergillosis in juvenile falcons (Falco spp.)
Aspergillosis is a common and life-threatening respiratory disease in raptors with acute and chronic courses. Among raptors, gyrfalcons (Falco rusticolus) and their hybrids are often declared to be highly susceptible with juvenile individuals being the most susceptible. However, species-and age-spec...
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ftunivgent:oai:archive.ugent.be:8560058 2023-06-11T04:11:35+02:00 Single tracheal inoculation of Aspergillus fumigatus conidia induced aspergillosis in juvenile falcons (Falco spp.) Fischer, Dominik Van Waeyenberghe, Lieven Failing, Klaus Martel, An Lierz, Michael 2017 application/pdf https://biblio.ugent.be/publication/8560058 http://hdl.handle.net/1854/LU-8560058 https://doi.org/10.1080/03079457.2017.1360470 https://biblio.ugent.be/publication/8560058/file/8560059 eng eng https://biblio.ugent.be/publication/8560058 http://hdl.handle.net/1854/LU-8560058 http://dx.doi.org/10.1080/03079457.2017.1360470 https://biblio.ugent.be/publication/8560058/file/8560059 No license (in copyright) info:eu-repo/semantics/restrictedAccess AVIAN PATHOLOGY ISSN: 0307-9457 ISSN: 1465-3338 Veterinary Sciences Birds of prey raptors mycosis fungal disease respiratory tract disease airway infection microbiology falconry PULMONARY ASPERGILLOSIS PATHOLOGICAL FINDINGS COTURNIX-JAPONICA CLINICAL SIGNS JAPANESE-QUAIL TURKEY POULTS SAUDI-ARABIA NEW-ZEALAND BIRDS INFECTIONS journalArticle info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2017 ftunivgent https://doi.org/10.1080/03079457.2017.1360470 2023-05-10T22:27:05Z Aspergillosis is a common and life-threatening respiratory disease in raptors with acute and chronic courses. Among raptors, gyrfalcons (Falco rusticolus) and their hybrids are often declared to be highly susceptible with juvenile individuals being the most susceptible. However, species-and age-specific experimental studies are lacking and minimal infective doses (IDs) for Aspergillus spp. conidia are unknown. Therefore, 8-week-old, healthy gyr-hybrid falcons (F. rusticolus X F. cherrug) (N = 18) were experimentally infected with Aspergillus fumigatus using a single intratracheal inoculation with varying dosages of conidia (10(2) to 10(7) conidia). Over 28 days, clinical signs were monitored as well as haematological and serological parameters. Following euthanasia, necropsy, histopathology, bacteriology, and mycology were performed. Re-isolated fungi were compared to the inoculum using microsatellite length polymorphisms. During the trial, clinical signs and dyspnoea correlated significantly with the ID. Necropsy revealed fungal lesions in the upper and lower airways of 10/18 inoculated falcons, but not in the control birds. In 9/18 inoculated falcons, fungal granulomas were confirmed in histopathology and A. fumigatus was re-isolated from these granulomas. Except one nasal isolate all re-isolated fungal strains were identical to the inoculum strain. Based on mycology and histopathology a minimal ID of 50% was calculated to be MID50% (+/- S.E.) = 10(4.52 +/- 0.67) for a single tracheal inoculation of A. fumigatus conidia. This study demonstrates for the first time that a single exposure is able to cause acute aspergillosis in juvenile falcons. Article in Journal/Newspaper Falco rusticolus Ghent University Academic Bibliography New Zealand Avian Pathology 47 1 33 46 |
institution |
Open Polar |
collection |
Ghent University Academic Bibliography |
op_collection_id |
ftunivgent |
language |
English |
topic |
Veterinary Sciences Birds of prey raptors mycosis fungal disease respiratory tract disease airway infection microbiology falconry PULMONARY ASPERGILLOSIS PATHOLOGICAL FINDINGS COTURNIX-JAPONICA CLINICAL SIGNS JAPANESE-QUAIL TURKEY POULTS SAUDI-ARABIA NEW-ZEALAND BIRDS INFECTIONS |
spellingShingle |
Veterinary Sciences Birds of prey raptors mycosis fungal disease respiratory tract disease airway infection microbiology falconry PULMONARY ASPERGILLOSIS PATHOLOGICAL FINDINGS COTURNIX-JAPONICA CLINICAL SIGNS JAPANESE-QUAIL TURKEY POULTS SAUDI-ARABIA NEW-ZEALAND BIRDS INFECTIONS Fischer, Dominik Van Waeyenberghe, Lieven Failing, Klaus Martel, An Lierz, Michael Single tracheal inoculation of Aspergillus fumigatus conidia induced aspergillosis in juvenile falcons (Falco spp.) |
topic_facet |
Veterinary Sciences Birds of prey raptors mycosis fungal disease respiratory tract disease airway infection microbiology falconry PULMONARY ASPERGILLOSIS PATHOLOGICAL FINDINGS COTURNIX-JAPONICA CLINICAL SIGNS JAPANESE-QUAIL TURKEY POULTS SAUDI-ARABIA NEW-ZEALAND BIRDS INFECTIONS |
description |
Aspergillosis is a common and life-threatening respiratory disease in raptors with acute and chronic courses. Among raptors, gyrfalcons (Falco rusticolus) and their hybrids are often declared to be highly susceptible with juvenile individuals being the most susceptible. However, species-and age-specific experimental studies are lacking and minimal infective doses (IDs) for Aspergillus spp. conidia are unknown. Therefore, 8-week-old, healthy gyr-hybrid falcons (F. rusticolus X F. cherrug) (N = 18) were experimentally infected with Aspergillus fumigatus using a single intratracheal inoculation with varying dosages of conidia (10(2) to 10(7) conidia). Over 28 days, clinical signs were monitored as well as haematological and serological parameters. Following euthanasia, necropsy, histopathology, bacteriology, and mycology were performed. Re-isolated fungi were compared to the inoculum using microsatellite length polymorphisms. During the trial, clinical signs and dyspnoea correlated significantly with the ID. Necropsy revealed fungal lesions in the upper and lower airways of 10/18 inoculated falcons, but not in the control birds. In 9/18 inoculated falcons, fungal granulomas were confirmed in histopathology and A. fumigatus was re-isolated from these granulomas. Except one nasal isolate all re-isolated fungal strains were identical to the inoculum strain. Based on mycology and histopathology a minimal ID of 50% was calculated to be MID50% (+/- S.E.) = 10(4.52 +/- 0.67) for a single tracheal inoculation of A. fumigatus conidia. This study demonstrates for the first time that a single exposure is able to cause acute aspergillosis in juvenile falcons. |
format |
Article in Journal/Newspaper |
author |
Fischer, Dominik Van Waeyenberghe, Lieven Failing, Klaus Martel, An Lierz, Michael |
author_facet |
Fischer, Dominik Van Waeyenberghe, Lieven Failing, Klaus Martel, An Lierz, Michael |
author_sort |
Fischer, Dominik |
title |
Single tracheal inoculation of Aspergillus fumigatus conidia induced aspergillosis in juvenile falcons (Falco spp.) |
title_short |
Single tracheal inoculation of Aspergillus fumigatus conidia induced aspergillosis in juvenile falcons (Falco spp.) |
title_full |
Single tracheal inoculation of Aspergillus fumigatus conidia induced aspergillosis in juvenile falcons (Falco spp.) |
title_fullStr |
Single tracheal inoculation of Aspergillus fumigatus conidia induced aspergillosis in juvenile falcons (Falco spp.) |
title_full_unstemmed |
Single tracheal inoculation of Aspergillus fumigatus conidia induced aspergillosis in juvenile falcons (Falco spp.) |
title_sort |
single tracheal inoculation of aspergillus fumigatus conidia induced aspergillosis in juvenile falcons (falco spp.) |
publishDate |
2017 |
url |
https://biblio.ugent.be/publication/8560058 http://hdl.handle.net/1854/LU-8560058 https://doi.org/10.1080/03079457.2017.1360470 https://biblio.ugent.be/publication/8560058/file/8560059 |
geographic |
New Zealand |
geographic_facet |
New Zealand |
genre |
Falco rusticolus |
genre_facet |
Falco rusticolus |
op_source |
AVIAN PATHOLOGY ISSN: 0307-9457 ISSN: 1465-3338 |
op_relation |
https://biblio.ugent.be/publication/8560058 http://hdl.handle.net/1854/LU-8560058 http://dx.doi.org/10.1080/03079457.2017.1360470 https://biblio.ugent.be/publication/8560058/file/8560059 |
op_rights |
No license (in copyright) info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1080/03079457.2017.1360470 |
container_title |
Avian Pathology |
container_volume |
47 |
container_issue |
1 |
container_start_page |
33 |
op_container_end_page |
46 |
_version_ |
1768386755711467520 |