Combined effects of temperature and salinity on fatty acid content and lipid damage in Antarctic phytoplankton
We investigated the effects of ocean warming and glacial melting on phytoplankton assemblage composition and physiology in coastal Antarctica by exposing assemblages to a 4 degrees C increase in seawater temperature (T) and a 4 psu decrease in salinity (S) with respect to ambient values in a 6-day m...
Published in: | Journal of Experimental Marine Biology and Ecology |
---|---|
Main Authors: | , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
2018
|
Subjects: | |
Online Access: | https://biblio.ugent.be/publication/8558633 http://hdl.handle.net/1854/LU-8558633 https://doi.org/10.1016/j.jembe.2018.03.004 https://biblio.ugent.be/publication/8558633/file/8558708 |
id |
ftunivgent:oai:archive.ugent.be:8558633 |
---|---|
record_format |
openpolar |
spelling |
ftunivgent:oai:archive.ugent.be:8558633 2023-06-11T04:06:13+02:00 Combined effects of temperature and salinity on fatty acid content and lipid damage in Antarctic phytoplankton Hernando, Marcelo Schloss, Irene R Almandoz, Gastón O Malanga, Gabriela Varela, Diana E De Troch, Marleen 2018 application/force-download https://biblio.ugent.be/publication/8558633 http://hdl.handle.net/1854/LU-8558633 https://doi.org/10.1016/j.jembe.2018.03.004 https://biblio.ugent.be/publication/8558633/file/8558708 eng eng https://biblio.ugent.be/publication/8558633 http://hdl.handle.net/1854/LU-8558633 http://dx.doi.org/10.1016/j.jembe.2018.03.004 https://biblio.ugent.be/publication/8558633/file/8558708 No license (in copyright) info:eu-repo/semantics/restrictedAccess JOURNAL OF EXPERIMENTAL MARINE BIOLOGY AND ECOLOGY ISSN: 0022-0981 Biology and Life Sciences Earth and Environmental Sciences High temperature Low salinity Unsaturated FAs TBARS Phytoplankton assemblage MEMBRANE-LIPIDS PHAEODACTYLUM-TRICORNUTUM BIOCHEMICAL-COMPOSITION COMMUNITY STRUCTURE DUNALIELLA-SALINA BATCH CULTURES SALT STRESS GROWTH-RATE MICROALGAE BACILLARIOPHYCEAE journalArticle info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2018 ftunivgent https://doi.org/10.1016/j.jembe.2018.03.004 2023-05-10T22:29:16Z We investigated the effects of ocean warming and glacial melting on phytoplankton assemblage composition and physiology in coastal Antarctica by exposing assemblages to a 4 degrees C increase in seawater temperature (T) and a 4 psu decrease in salinity (S) with respect to ambient values in a 6-day microcosm experiment. Seawater samples from Potter Cove in King George Island (Antarctica) were placed in outdoor microcosms and exposed to four treatments: ambient S-ambient T (S0T0, control), low S-ambient T (S-TO), ambient S-high T (SOT +), and low thigh T (S-T +). The relative abundance of unsaturated fatty acids (UFAs) 20:5 omega 3,18:4 omega 3 and 16: ha in relation to saturated FAs (14:0 and 16:0) significantly increased in all treatments at 24 h, compared to the control. At the same time, we detected a significant increase in the production of Thiobarbituric Acid Reactive Substances (TEARS), used as a proxy for lipid damage, in the S-TO and the SOT + treatments. In contrast, in S-T +, concentrations of TBARS remained significantly lower than in the control throughout the experiment. Although phytoplankton species composition did not change during the experiment, an increase in the relative abundance of diatoms ( > 20 mu m) was found in all treatments compared to the control at 24 h, with no further changes during the rest of the experiment. Furthermore, the relative abundance of small diatoms (10-20 mu m) increased only in SOT +, and small prasinophytes decreased at S-T + at the end of the incubation period. Our results show a stable unsaturated to saturated FA ratio under the synergistic effects of high temperature and lower salinity, which may help protect phytoplankton cells from lipid damage. When phytoplankton assemblages were exposed to high temperature or low salinity, separately, the proportion of unsaturated FAs increased after 48 h. This increase in FAs resulted in greater lipid damage, which could be potentially avoided, as shown by previous studies, by antioxidant responses or changes in ... Article in Journal/Newspaper Antarc* Antarctic Antarctica King George Island Ghent University Academic Bibliography Antarctic King George Island Potter Cove Journal of Experimental Marine Biology and Ecology 503 120 128 |
institution |
Open Polar |
collection |
Ghent University Academic Bibliography |
op_collection_id |
ftunivgent |
language |
English |
topic |
Biology and Life Sciences Earth and Environmental Sciences High temperature Low salinity Unsaturated FAs TBARS Phytoplankton assemblage MEMBRANE-LIPIDS PHAEODACTYLUM-TRICORNUTUM BIOCHEMICAL-COMPOSITION COMMUNITY STRUCTURE DUNALIELLA-SALINA BATCH CULTURES SALT STRESS GROWTH-RATE MICROALGAE BACILLARIOPHYCEAE |
spellingShingle |
Biology and Life Sciences Earth and Environmental Sciences High temperature Low salinity Unsaturated FAs TBARS Phytoplankton assemblage MEMBRANE-LIPIDS PHAEODACTYLUM-TRICORNUTUM BIOCHEMICAL-COMPOSITION COMMUNITY STRUCTURE DUNALIELLA-SALINA BATCH CULTURES SALT STRESS GROWTH-RATE MICROALGAE BACILLARIOPHYCEAE Hernando, Marcelo Schloss, Irene R Almandoz, Gastón O Malanga, Gabriela Varela, Diana E De Troch, Marleen Combined effects of temperature and salinity on fatty acid content and lipid damage in Antarctic phytoplankton |
topic_facet |
Biology and Life Sciences Earth and Environmental Sciences High temperature Low salinity Unsaturated FAs TBARS Phytoplankton assemblage MEMBRANE-LIPIDS PHAEODACTYLUM-TRICORNUTUM BIOCHEMICAL-COMPOSITION COMMUNITY STRUCTURE DUNALIELLA-SALINA BATCH CULTURES SALT STRESS GROWTH-RATE MICROALGAE BACILLARIOPHYCEAE |
description |
We investigated the effects of ocean warming and glacial melting on phytoplankton assemblage composition and physiology in coastal Antarctica by exposing assemblages to a 4 degrees C increase in seawater temperature (T) and a 4 psu decrease in salinity (S) with respect to ambient values in a 6-day microcosm experiment. Seawater samples from Potter Cove in King George Island (Antarctica) were placed in outdoor microcosms and exposed to four treatments: ambient S-ambient T (S0T0, control), low S-ambient T (S-TO), ambient S-high T (SOT +), and low thigh T (S-T +). The relative abundance of unsaturated fatty acids (UFAs) 20:5 omega 3,18:4 omega 3 and 16: ha in relation to saturated FAs (14:0 and 16:0) significantly increased in all treatments at 24 h, compared to the control. At the same time, we detected a significant increase in the production of Thiobarbituric Acid Reactive Substances (TEARS), used as a proxy for lipid damage, in the S-TO and the SOT + treatments. In contrast, in S-T +, concentrations of TBARS remained significantly lower than in the control throughout the experiment. Although phytoplankton species composition did not change during the experiment, an increase in the relative abundance of diatoms ( > 20 mu m) was found in all treatments compared to the control at 24 h, with no further changes during the rest of the experiment. Furthermore, the relative abundance of small diatoms (10-20 mu m) increased only in SOT +, and small prasinophytes decreased at S-T + at the end of the incubation period. Our results show a stable unsaturated to saturated FA ratio under the synergistic effects of high temperature and lower salinity, which may help protect phytoplankton cells from lipid damage. When phytoplankton assemblages were exposed to high temperature or low salinity, separately, the proportion of unsaturated FAs increased after 48 h. This increase in FAs resulted in greater lipid damage, which could be potentially avoided, as shown by previous studies, by antioxidant responses or changes in ... |
format |
Article in Journal/Newspaper |
author |
Hernando, Marcelo Schloss, Irene R Almandoz, Gastón O Malanga, Gabriela Varela, Diana E De Troch, Marleen |
author_facet |
Hernando, Marcelo Schloss, Irene R Almandoz, Gastón O Malanga, Gabriela Varela, Diana E De Troch, Marleen |
author_sort |
Hernando, Marcelo |
title |
Combined effects of temperature and salinity on fatty acid content and lipid damage in Antarctic phytoplankton |
title_short |
Combined effects of temperature and salinity on fatty acid content and lipid damage in Antarctic phytoplankton |
title_full |
Combined effects of temperature and salinity on fatty acid content and lipid damage in Antarctic phytoplankton |
title_fullStr |
Combined effects of temperature and salinity on fatty acid content and lipid damage in Antarctic phytoplankton |
title_full_unstemmed |
Combined effects of temperature and salinity on fatty acid content and lipid damage in Antarctic phytoplankton |
title_sort |
combined effects of temperature and salinity on fatty acid content and lipid damage in antarctic phytoplankton |
publishDate |
2018 |
url |
https://biblio.ugent.be/publication/8558633 http://hdl.handle.net/1854/LU-8558633 https://doi.org/10.1016/j.jembe.2018.03.004 https://biblio.ugent.be/publication/8558633/file/8558708 |
geographic |
Antarctic King George Island Potter Cove |
geographic_facet |
Antarctic King George Island Potter Cove |
genre |
Antarc* Antarctic Antarctica King George Island |
genre_facet |
Antarc* Antarctic Antarctica King George Island |
op_source |
JOURNAL OF EXPERIMENTAL MARINE BIOLOGY AND ECOLOGY ISSN: 0022-0981 |
op_relation |
https://biblio.ugent.be/publication/8558633 http://hdl.handle.net/1854/LU-8558633 http://dx.doi.org/10.1016/j.jembe.2018.03.004 https://biblio.ugent.be/publication/8558633/file/8558708 |
op_rights |
No license (in copyright) info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1016/j.jembe.2018.03.004 |
container_title |
Journal of Experimental Marine Biology and Ecology |
container_volume |
503 |
container_start_page |
120 |
op_container_end_page |
128 |
_version_ |
1768378028309610496 |