Experimental infection model for vibriosis in Dover sole (Solea solea) larvae as an aid in studying its pathogenesis and alternative treatments
Severe economic losses due to diseases in marine larviculture may be linked to vibriosis. To better understand the pathogenesis of vibriosis and evaluate new ways to prevent and combat this important disease, there is a great need for reliable and reproducible experimental infection models. The pres...
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ftunivgent:oai:archive.ugent.be:8552080 2023-06-11T04:11:48+02:00 Experimental infection model for vibriosis in Dover sole (Solea solea) larvae as an aid in studying its pathogenesis and alternative treatments De Swaef, Evelien Vercauteren, Maaike Duchateau, Luc Haesebrouck, Freddy Decostere, Annemie 2018 application/pdf https://biblio.ugent.be/publication/8552080 http://hdl.handle.net/1854/LU-8552080 https://doi.org/10.1186/s13567-018-0520-3 https://biblio.ugent.be/publication/8552080/file/8552081 eng eng https://biblio.ugent.be/publication/8552080 http://hdl.handle.net/1854/LU-8552080 http://dx.doi.org/10.1186/s13567-018-0520-3 https://biblio.ugent.be/publication/8552080/file/8552081 Creative Commons Attribution 4.0 International Public License (CC-BY 4.0) info:eu-repo/semantics/openAccess VETERINARY RESEARCH ISSN: 0928-4249 ISSN: 1297-9716 Veterinary Sciences Biology and Life Sciences TURBOT SCOPHTHALMUS-MAXIMUS DICENTRARCHUS-LABRAX L LACTIC-ACID BACTERIA COD GADUS-MORHUA YOLK-SAC LARVAE LISTONELLA-ANGUILLARUM PROBIOTIC BACTERIA SENEGALENSIS KAUP CHALLENGE TEST FOOD-CHAIN journalArticle info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2018 ftunivgent https://doi.org/10.1186/s13567-018-0520-3 2023-05-10T22:26:51Z Severe economic losses due to diseases in marine larviculture may be linked to vibriosis. To better understand the pathogenesis of vibriosis and evaluate new ways to prevent and combat this important disease, there is a great need for reliable and reproducible experimental infection models. The present study aimed at developing a challenge model for vibriosis in Dover sole larvae and testing its applicability to study the effect of the probiotic treatment. For that purpose, larvae were challenged at 10 days post hatching with Vibrio anguillarum WT, V. anguillarum HI610 or V. harveyi WT. Following administration of V. anguillarum WT via immersion at 1 × 107 colony forming units/mL, a larval mortality of 50% was observed at 17 days post-inoculation. In a next step, the probiotic potential of 371 isolates retrieved from Dover sole was assessed by screening for their inhibitory effects against Vibrio spp. and absence of haemolytic activity. One remaining isolate (V. proteolyticus) and V. lentus, known for its protective characteristics in seabass larvae, were further tested in vivo by means of the pinpointed experimental infection model. Neither isolate provided via the water or feed proved to be protective for the Dover sole larvae against challenge with V. anguillarum WT. This developed challenge model constitutes a firm basis to expedite basic and applied research regarding the pathogenesis and treatment of vibriosis as well as for studying the impact of (a)biotic components on larval health. Article in Journal/Newspaper Gadus morhua Scophthalmus maximus Turbot Ghent University Academic Bibliography Dover ENVELOPE(-55.753,-55.753,-83.777,-83.777) Veterinary Research 49 1 |
institution |
Open Polar |
collection |
Ghent University Academic Bibliography |
op_collection_id |
ftunivgent |
language |
English |
topic |
Veterinary Sciences Biology and Life Sciences TURBOT SCOPHTHALMUS-MAXIMUS DICENTRARCHUS-LABRAX L LACTIC-ACID BACTERIA COD GADUS-MORHUA YOLK-SAC LARVAE LISTONELLA-ANGUILLARUM PROBIOTIC BACTERIA SENEGALENSIS KAUP CHALLENGE TEST FOOD-CHAIN |
spellingShingle |
Veterinary Sciences Biology and Life Sciences TURBOT SCOPHTHALMUS-MAXIMUS DICENTRARCHUS-LABRAX L LACTIC-ACID BACTERIA COD GADUS-MORHUA YOLK-SAC LARVAE LISTONELLA-ANGUILLARUM PROBIOTIC BACTERIA SENEGALENSIS KAUP CHALLENGE TEST FOOD-CHAIN De Swaef, Evelien Vercauteren, Maaike Duchateau, Luc Haesebrouck, Freddy Decostere, Annemie Experimental infection model for vibriosis in Dover sole (Solea solea) larvae as an aid in studying its pathogenesis and alternative treatments |
topic_facet |
Veterinary Sciences Biology and Life Sciences TURBOT SCOPHTHALMUS-MAXIMUS DICENTRARCHUS-LABRAX L LACTIC-ACID BACTERIA COD GADUS-MORHUA YOLK-SAC LARVAE LISTONELLA-ANGUILLARUM PROBIOTIC BACTERIA SENEGALENSIS KAUP CHALLENGE TEST FOOD-CHAIN |
description |
Severe economic losses due to diseases in marine larviculture may be linked to vibriosis. To better understand the pathogenesis of vibriosis and evaluate new ways to prevent and combat this important disease, there is a great need for reliable and reproducible experimental infection models. The present study aimed at developing a challenge model for vibriosis in Dover sole larvae and testing its applicability to study the effect of the probiotic treatment. For that purpose, larvae were challenged at 10 days post hatching with Vibrio anguillarum WT, V. anguillarum HI610 or V. harveyi WT. Following administration of V. anguillarum WT via immersion at 1 × 107 colony forming units/mL, a larval mortality of 50% was observed at 17 days post-inoculation. In a next step, the probiotic potential of 371 isolates retrieved from Dover sole was assessed by screening for their inhibitory effects against Vibrio spp. and absence of haemolytic activity. One remaining isolate (V. proteolyticus) and V. lentus, known for its protective characteristics in seabass larvae, were further tested in vivo by means of the pinpointed experimental infection model. Neither isolate provided via the water or feed proved to be protective for the Dover sole larvae against challenge with V. anguillarum WT. This developed challenge model constitutes a firm basis to expedite basic and applied research regarding the pathogenesis and treatment of vibriosis as well as for studying the impact of (a)biotic components on larval health. |
format |
Article in Journal/Newspaper |
author |
De Swaef, Evelien Vercauteren, Maaike Duchateau, Luc Haesebrouck, Freddy Decostere, Annemie |
author_facet |
De Swaef, Evelien Vercauteren, Maaike Duchateau, Luc Haesebrouck, Freddy Decostere, Annemie |
author_sort |
De Swaef, Evelien |
title |
Experimental infection model for vibriosis in Dover sole (Solea solea) larvae as an aid in studying its pathogenesis and alternative treatments |
title_short |
Experimental infection model for vibriosis in Dover sole (Solea solea) larvae as an aid in studying its pathogenesis and alternative treatments |
title_full |
Experimental infection model for vibriosis in Dover sole (Solea solea) larvae as an aid in studying its pathogenesis and alternative treatments |
title_fullStr |
Experimental infection model for vibriosis in Dover sole (Solea solea) larvae as an aid in studying its pathogenesis and alternative treatments |
title_full_unstemmed |
Experimental infection model for vibriosis in Dover sole (Solea solea) larvae as an aid in studying its pathogenesis and alternative treatments |
title_sort |
experimental infection model for vibriosis in dover sole (solea solea) larvae as an aid in studying its pathogenesis and alternative treatments |
publishDate |
2018 |
url |
https://biblio.ugent.be/publication/8552080 http://hdl.handle.net/1854/LU-8552080 https://doi.org/10.1186/s13567-018-0520-3 https://biblio.ugent.be/publication/8552080/file/8552081 |
long_lat |
ENVELOPE(-55.753,-55.753,-83.777,-83.777) |
geographic |
Dover |
geographic_facet |
Dover |
genre |
Gadus morhua Scophthalmus maximus Turbot |
genre_facet |
Gadus morhua Scophthalmus maximus Turbot |
op_source |
VETERINARY RESEARCH ISSN: 0928-4249 ISSN: 1297-9716 |
op_relation |
https://biblio.ugent.be/publication/8552080 http://hdl.handle.net/1854/LU-8552080 http://dx.doi.org/10.1186/s13567-018-0520-3 https://biblio.ugent.be/publication/8552080/file/8552081 |
op_rights |
Creative Commons Attribution 4.0 International Public License (CC-BY 4.0) info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1186/s13567-018-0520-3 |
container_title |
Veterinary Research |
container_volume |
49 |
container_issue |
1 |
_version_ |
1768387128319803392 |