High accumulation in tobacco seeds of hemagglutinin antigen from avian (H5N1) influenza
Tobacco seeds can be used as a cost effective system for production of recombinant vaccines. Avian influenza is an important respiratory pathogen that causes a high degree of mortality and becomes a serious threat for the poultry industry. A safe vaccine against avian flu produced at low cost could...
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ftunivgent:oai:archive.ugent.be:8540272 2023-06-11T04:10:24+02:00 High accumulation in tobacco seeds of hemagglutinin antigen from avian (H5N1) influenza Ceballo, Yanaysi Tiel, Kenia López, Alina Cabrera, Gleysin Pérez, Marlene Ramos, Osmany Rosabal, Yamilka Montero, Carlos Menassa, Rima Depicker, Anna Hernández, Abel 2017 application/pdf https://biblio.ugent.be/publication/8540272 http://hdl.handle.net/1854/LU-8540272 https://doi.org/10.1007/s11248-017-0047-9 https://biblio.ugent.be/publication/8540272/file/8540273 eng eng https://biblio.ugent.be/publication/8540272 http://hdl.handle.net/1854/LU-8540272 http://dx.doi.org/10.1007/s11248-017-0047-9 https://biblio.ugent.be/publication/8540272/file/8540273 No license (in copyright) info:eu-repo/semantics/restrictedAccess TRANSGENIC RESEARCH ISSN: 0962-8819 ISSN: 1573-9368 Biology and Life Sciences VIRUS-LIKE PARTICLES BROADLY NEUTRALIZING ANTIBODIES N-GLYCANS NICOTIANA-BENTHAMIANA PROTECTS CHICKENS IMMUNE-RESPONSE CHALLENGE INFECTION ARABIDOPSIS SEEDS VACCINE ANTIGEN A H5N1 Avian influenza Tobacco seed Hemagglutinin Phaseolin promoter journalArticle info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2017 ftunivgent https://doi.org/10.1007/s11248-017-0047-9 2023-05-10T22:41:48Z Tobacco seeds can be used as a cost effective system for production of recombinant vaccines. Avian influenza is an important respiratory pathogen that causes a high degree of mortality and becomes a serious threat for the poultry industry. A safe vaccine against avian flu produced at low cost could help to prevent future outbreaks. We have genetically engineered tobacco plants to express extracellular domain of hemagglutinin protein from H5N1 avian influenza virus as an inexpensive alternative for production purposes. Two regulatory sequences of seed storage protein genes from Phaseolus vulgaris L. were used to direct the expression, yielding 3.0 mg of the viral antigen per g of seeds. The production and stability of seed-produced recombinant HA protein was characterized by different molecular techniques. The aqueous extract of tobacco seed proteins was used for subcutaneous immunization of chickens, which developed antibodies that inhibited the agglutination of erythrocytes after the second application of the antigen. The feasibility of using tobacco seeds as a vaccine carrier is discussed. Article in Journal/Newspaper Avian flu Ghent University Academic Bibliography Transgenic Research 26 6 775 789 |
institution |
Open Polar |
collection |
Ghent University Academic Bibliography |
op_collection_id |
ftunivgent |
language |
English |
topic |
Biology and Life Sciences VIRUS-LIKE PARTICLES BROADLY NEUTRALIZING ANTIBODIES N-GLYCANS NICOTIANA-BENTHAMIANA PROTECTS CHICKENS IMMUNE-RESPONSE CHALLENGE INFECTION ARABIDOPSIS SEEDS VACCINE ANTIGEN A H5N1 Avian influenza Tobacco seed Hemagglutinin Phaseolin promoter |
spellingShingle |
Biology and Life Sciences VIRUS-LIKE PARTICLES BROADLY NEUTRALIZING ANTIBODIES N-GLYCANS NICOTIANA-BENTHAMIANA PROTECTS CHICKENS IMMUNE-RESPONSE CHALLENGE INFECTION ARABIDOPSIS SEEDS VACCINE ANTIGEN A H5N1 Avian influenza Tobacco seed Hemagglutinin Phaseolin promoter Ceballo, Yanaysi Tiel, Kenia López, Alina Cabrera, Gleysin Pérez, Marlene Ramos, Osmany Rosabal, Yamilka Montero, Carlos Menassa, Rima Depicker, Anna Hernández, Abel High accumulation in tobacco seeds of hemagglutinin antigen from avian (H5N1) influenza |
topic_facet |
Biology and Life Sciences VIRUS-LIKE PARTICLES BROADLY NEUTRALIZING ANTIBODIES N-GLYCANS NICOTIANA-BENTHAMIANA PROTECTS CHICKENS IMMUNE-RESPONSE CHALLENGE INFECTION ARABIDOPSIS SEEDS VACCINE ANTIGEN A H5N1 Avian influenza Tobacco seed Hemagglutinin Phaseolin promoter |
description |
Tobacco seeds can be used as a cost effective system for production of recombinant vaccines. Avian influenza is an important respiratory pathogen that causes a high degree of mortality and becomes a serious threat for the poultry industry. A safe vaccine against avian flu produced at low cost could help to prevent future outbreaks. We have genetically engineered tobacco plants to express extracellular domain of hemagglutinin protein from H5N1 avian influenza virus as an inexpensive alternative for production purposes. Two regulatory sequences of seed storage protein genes from Phaseolus vulgaris L. were used to direct the expression, yielding 3.0 mg of the viral antigen per g of seeds. The production and stability of seed-produced recombinant HA protein was characterized by different molecular techniques. The aqueous extract of tobacco seed proteins was used for subcutaneous immunization of chickens, which developed antibodies that inhibited the agglutination of erythrocytes after the second application of the antigen. The feasibility of using tobacco seeds as a vaccine carrier is discussed. |
format |
Article in Journal/Newspaper |
author |
Ceballo, Yanaysi Tiel, Kenia López, Alina Cabrera, Gleysin Pérez, Marlene Ramos, Osmany Rosabal, Yamilka Montero, Carlos Menassa, Rima Depicker, Anna Hernández, Abel |
author_facet |
Ceballo, Yanaysi Tiel, Kenia López, Alina Cabrera, Gleysin Pérez, Marlene Ramos, Osmany Rosabal, Yamilka Montero, Carlos Menassa, Rima Depicker, Anna Hernández, Abel |
author_sort |
Ceballo, Yanaysi |
title |
High accumulation in tobacco seeds of hemagglutinin antigen from avian (H5N1) influenza |
title_short |
High accumulation in tobacco seeds of hemagglutinin antigen from avian (H5N1) influenza |
title_full |
High accumulation in tobacco seeds of hemagglutinin antigen from avian (H5N1) influenza |
title_fullStr |
High accumulation in tobacco seeds of hemagglutinin antigen from avian (H5N1) influenza |
title_full_unstemmed |
High accumulation in tobacco seeds of hemagglutinin antigen from avian (H5N1) influenza |
title_sort |
high accumulation in tobacco seeds of hemagglutinin antigen from avian (h5n1) influenza |
publishDate |
2017 |
url |
https://biblio.ugent.be/publication/8540272 http://hdl.handle.net/1854/LU-8540272 https://doi.org/10.1007/s11248-017-0047-9 https://biblio.ugent.be/publication/8540272/file/8540273 |
genre |
Avian flu |
genre_facet |
Avian flu |
op_source |
TRANSGENIC RESEARCH ISSN: 0962-8819 ISSN: 1573-9368 |
op_relation |
https://biblio.ugent.be/publication/8540272 http://hdl.handle.net/1854/LU-8540272 http://dx.doi.org/10.1007/s11248-017-0047-9 https://biblio.ugent.be/publication/8540272/file/8540273 |
op_rights |
No license (in copyright) info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1007/s11248-017-0047-9 |
container_title |
Transgenic Research |
container_volume |
26 |
container_issue |
6 |
container_start_page |
775 |
op_container_end_page |
789 |
_version_ |
1768384767850446848 |