Development of a reliable experimental set-up for Dover sole larvae Solea solea L. and exploring the possibility of implementing this housing system in a gnotobiotic model
Due to the increasing importance of the aquaculture sector, diversification in the number of cultured species imposes itself. Dover sole Solea solea L. is put forward as an important new aquaculture candidate due to its high market value and high flesh quality. However, as for many other fish specie...
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ftunivgent:oai:archive.ugent.be:8538555 2023-06-11T04:16:29+02:00 Development of a reliable experimental set-up for Dover sole larvae Solea solea L. and exploring the possibility of implementing this housing system in a gnotobiotic model De Swaef, Evelien Demeestere, Kristof Boon, Nico Van Den Broeck, Wim Haesebrouck, Freddy Decostere, Annemie 2017 application/pdf https://biblio.ugent.be/publication/8538555 http://hdl.handle.net/1854/LU-8538555 https://doi.org/10.1016/j.rvsc.2017.07.025 https://biblio.ugent.be/publication/8538555/file/8538561 eng eng https://biblio.ugent.be/publication/8538555 http://hdl.handle.net/1854/LU-8538555 http://dx.doi.org/10.1016/j.rvsc.2017.07.025 https://biblio.ugent.be/publication/8538555/file/8538561 No license (in copyright) info:eu-repo/semantics/restrictedAccess RESEARCH IN VETERINARY SCIENCE ISSN: 0034-5288 Biology and Life Sciences Veterinary Sciences Disinfection Dover sole Egg Housing Larvae Well plate SCOPHTHALMUS-MAXIMUS L BACTERIAL CHALLENGE TEST EUROPEAN FISH LARVAE MARINE FISH SURFACE DISINFECTION VIBRIO-ANGUILLARUM ATLANTIC HALIBUT TOXIC DINOFLAGELLATE SKELETAL ANOMALIES HYDROGEN-PEROXIDE journalArticle info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2017 ftunivgent https://doi.org/10.1016/j.rvsc.2017.07.025 2023-05-10T22:41:48Z Due to the increasing importance of the aquaculture sector, diversification in the number of cultured species imposes itself. Dover sole Solea solea L. is put forward as an important new aquaculture candidate due to its high market value and high flesh quality. However, as for many other fish species, sole production is hampered by amongst others high susceptibility to diseases and larval mortality, rendering the need for more research in this area. In this respect, in first instance, a housing system for Dover sole larvae was pinpointed by keeping the animals individually in 24-well plates for 26 days with good survival rates and initiating metamorphosis. This ensures a standardised and reliable experimental set-up in which the possible death of one larva has no effect on the other larvae, rendering experiments adopting such a system more reproducible. In addition to proving valuable in many other applications, this multi well system constitutes a firm basis to enable the gnotobiotic rearing of larvae, which hitherto is non-existing for Dover sole. In this respect, secondly, a large number of disinfection protocols were tested, making use of widely employed disinfectants as hydrogen peroxide, glutaraldehyde and/or ozone whether or not combined with a mixture of antimicrobial agents for 24 h. Although none of the tested protocols was sufficient to reproducibly generate a gnotobiotic model, the combination of glutaraldehyde and hydrogen peroxide resulted in hatchable, bacteria-free eggs in some cases. Article in Journal/Newspaper Scophthalmus maximus Ghent University Academic Bibliography Dover ENVELOPE(-55.753,-55.753,-83.777,-83.777) Research in Veterinary Science 115 418 424 |
institution |
Open Polar |
collection |
Ghent University Academic Bibliography |
op_collection_id |
ftunivgent |
language |
English |
topic |
Biology and Life Sciences Veterinary Sciences Disinfection Dover sole Egg Housing Larvae Well plate SCOPHTHALMUS-MAXIMUS L BACTERIAL CHALLENGE TEST EUROPEAN FISH LARVAE MARINE FISH SURFACE DISINFECTION VIBRIO-ANGUILLARUM ATLANTIC HALIBUT TOXIC DINOFLAGELLATE SKELETAL ANOMALIES HYDROGEN-PEROXIDE |
spellingShingle |
Biology and Life Sciences Veterinary Sciences Disinfection Dover sole Egg Housing Larvae Well plate SCOPHTHALMUS-MAXIMUS L BACTERIAL CHALLENGE TEST EUROPEAN FISH LARVAE MARINE FISH SURFACE DISINFECTION VIBRIO-ANGUILLARUM ATLANTIC HALIBUT TOXIC DINOFLAGELLATE SKELETAL ANOMALIES HYDROGEN-PEROXIDE De Swaef, Evelien Demeestere, Kristof Boon, Nico Van Den Broeck, Wim Haesebrouck, Freddy Decostere, Annemie Development of a reliable experimental set-up for Dover sole larvae Solea solea L. and exploring the possibility of implementing this housing system in a gnotobiotic model |
topic_facet |
Biology and Life Sciences Veterinary Sciences Disinfection Dover sole Egg Housing Larvae Well plate SCOPHTHALMUS-MAXIMUS L BACTERIAL CHALLENGE TEST EUROPEAN FISH LARVAE MARINE FISH SURFACE DISINFECTION VIBRIO-ANGUILLARUM ATLANTIC HALIBUT TOXIC DINOFLAGELLATE SKELETAL ANOMALIES HYDROGEN-PEROXIDE |
description |
Due to the increasing importance of the aquaculture sector, diversification in the number of cultured species imposes itself. Dover sole Solea solea L. is put forward as an important new aquaculture candidate due to its high market value and high flesh quality. However, as for many other fish species, sole production is hampered by amongst others high susceptibility to diseases and larval mortality, rendering the need for more research in this area. In this respect, in first instance, a housing system for Dover sole larvae was pinpointed by keeping the animals individually in 24-well plates for 26 days with good survival rates and initiating metamorphosis. This ensures a standardised and reliable experimental set-up in which the possible death of one larva has no effect on the other larvae, rendering experiments adopting such a system more reproducible. In addition to proving valuable in many other applications, this multi well system constitutes a firm basis to enable the gnotobiotic rearing of larvae, which hitherto is non-existing for Dover sole. In this respect, secondly, a large number of disinfection protocols were tested, making use of widely employed disinfectants as hydrogen peroxide, glutaraldehyde and/or ozone whether or not combined with a mixture of antimicrobial agents for 24 h. Although none of the tested protocols was sufficient to reproducibly generate a gnotobiotic model, the combination of glutaraldehyde and hydrogen peroxide resulted in hatchable, bacteria-free eggs in some cases. |
format |
Article in Journal/Newspaper |
author |
De Swaef, Evelien Demeestere, Kristof Boon, Nico Van Den Broeck, Wim Haesebrouck, Freddy Decostere, Annemie |
author_facet |
De Swaef, Evelien Demeestere, Kristof Boon, Nico Van Den Broeck, Wim Haesebrouck, Freddy Decostere, Annemie |
author_sort |
De Swaef, Evelien |
title |
Development of a reliable experimental set-up for Dover sole larvae Solea solea L. and exploring the possibility of implementing this housing system in a gnotobiotic model |
title_short |
Development of a reliable experimental set-up for Dover sole larvae Solea solea L. and exploring the possibility of implementing this housing system in a gnotobiotic model |
title_full |
Development of a reliable experimental set-up for Dover sole larvae Solea solea L. and exploring the possibility of implementing this housing system in a gnotobiotic model |
title_fullStr |
Development of a reliable experimental set-up for Dover sole larvae Solea solea L. and exploring the possibility of implementing this housing system in a gnotobiotic model |
title_full_unstemmed |
Development of a reliable experimental set-up for Dover sole larvae Solea solea L. and exploring the possibility of implementing this housing system in a gnotobiotic model |
title_sort |
development of a reliable experimental set-up for dover sole larvae solea solea l. and exploring the possibility of implementing this housing system in a gnotobiotic model |
publishDate |
2017 |
url |
https://biblio.ugent.be/publication/8538555 http://hdl.handle.net/1854/LU-8538555 https://doi.org/10.1016/j.rvsc.2017.07.025 https://biblio.ugent.be/publication/8538555/file/8538561 |
long_lat |
ENVELOPE(-55.753,-55.753,-83.777,-83.777) |
geographic |
Dover |
geographic_facet |
Dover |
genre |
Scophthalmus maximus |
genre_facet |
Scophthalmus maximus |
op_source |
RESEARCH IN VETERINARY SCIENCE ISSN: 0034-5288 |
op_relation |
https://biblio.ugent.be/publication/8538555 http://hdl.handle.net/1854/LU-8538555 http://dx.doi.org/10.1016/j.rvsc.2017.07.025 https://biblio.ugent.be/publication/8538555/file/8538561 |
op_rights |
No license (in copyright) info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1016/j.rvsc.2017.07.025 |
container_title |
Research in Veterinary Science |
container_volume |
115 |
container_start_page |
418 |
op_container_end_page |
424 |
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1768374786488008704 |