Development of a reliable experimental set-up for Dover sole larvae Solea solea L. and exploring the possibility of implementing this housing system in a gnotobiotic model

Due to the increasing importance of the aquaculture sector, diversification in the number of cultured species imposes itself. Dover sole Solea solea L. is put forward as an important new aquaculture candidate due to its high market value and high flesh quality. However, as for many other fish specie...

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Bibliographic Details
Published in:Research in Veterinary Science
Main Authors: De Swaef, Evelien, Demeestere, Kristof, Boon, Nico, Van Den Broeck, Wim, Haesebrouck, Freddy, Decostere, Annemie
Format: Article in Journal/Newspaper
Language:English
Published: 2017
Subjects:
Biology and Life Sciences
Veterinary Sciences
Disinfection
Dover sole
Egg
Housing
Larvae
Well plate
SCOPHTHALMUS-MAXIMUS L
BACTERIAL CHALLENGE TEST
EUROPEAN FISH LARVAE
MARINE FISH
SURFACE DISINFECTION
VIBRIO-ANGUILLARUM
ATLANTIC HALIBUT
TOXIC DINOFLAGELLATE
SKELETAL ANOMALIES
HYDROGEN-PEROXIDE
Dover
Scophthalmus maximus
Online Access:https://biblio.ugent.be/publication/8538555
http://hdl.handle.net/1854/LU-8538555
https://doi.org/10.1016/j.rvsc.2017.07.025
https://biblio.ugent.be/publication/8538555/file/8538561
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Summary:Due to the increasing importance of the aquaculture sector, diversification in the number of cultured species imposes itself. Dover sole Solea solea L. is put forward as an important new aquaculture candidate due to its high market value and high flesh quality. However, as for many other fish species, sole production is hampered by amongst others high susceptibility to diseases and larval mortality, rendering the need for more research in this area. In this respect, in first instance, a housing system for Dover sole larvae was pinpointed by keeping the animals individually in 24-well plates for 26 days with good survival rates and initiating metamorphosis. This ensures a standardised and reliable experimental set-up in which the possible death of one larva has no effect on the other larvae, rendering experiments adopting such a system more reproducible. In addition to proving valuable in many other applications, this multi well system constitutes a firm basis to enable the gnotobiotic rearing of larvae, which hitherto is non-existing for Dover sole. In this respect, secondly, a large number of disinfection protocols were tested, making use of widely employed disinfectants as hydrogen peroxide, glutaraldehyde and/or ozone whether or not combined with a mixture of antimicrobial agents for 24 h. Although none of the tested protocols was sufficient to reproducibly generate a gnotobiotic model, the combination of glutaraldehyde and hydrogen peroxide resulted in hatchable, bacteria-free eggs in some cases.

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