Induced expression of selected plant defence related genes in pot azalea, Rhododendron simsii hybrid
A set of putative marker genes to study plant defense responses against Polyphagotarsonemus latus, a key pest in the production of Rhododendron simsii hybrids, was selected and validated. Genes belonged to the biosynthetic pathway of phytohormones jasmonic acid (JA) (RsLOX, RsAOS, RsAOC, RsOPR3 and...
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ftunivgent:oai:archive.ugent.be:8533328 2023-06-11T04:17:39+02:00 Induced expression of selected plant defence related genes in pot azalea, Rhododendron simsii hybrid Luypaert, Gil Witters, Johan Van Huylenbroeck, Johan De Clercq, Patrick De Riek, Jan De Keyser, Ellen 2017 application/pdf https://biblio.ugent.be/publication/8533328 http://hdl.handle.net/1854/LU-8533328 https://doi.org/10.1007/s10681-017-2010-5 https://biblio.ugent.be/publication/8533328/file/8533331 eng eng https://biblio.ugent.be/publication/8533328 http://hdl.handle.net/1854/LU-8533328 http://dx.doi.org/10.1007/s10681-017-2010-5 https://biblio.ugent.be/publication/8533328/file/8533331 No license (in copyright) info:eu-repo/semantics/restrictedAccess EUPHYTICA ISSN: 0014-2336 ISSN: 1573-5060 Biology and Life Sciences Jasmonic acid (JA) Methyl jasmonate (MeJA) Polyphagotarsonemus latus Rhododendron simsii hybrids RT-qPCR RNA extraction quality Salicylic acid (SA) LATUS ACARI TARSONEMIDAE TIME RT-PCR ACID CARBOXYL METHYLTRANSFERASE LEAF POLYPHENOL OXIDASE HOT BORATE METHOD HIGH-QUALITY RNA JASMONIC-ACID BROAD MITE POLYPHAGOTARSONEMUS-LATUS ARABIDOPSIS-THALIANA journalArticle info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2017 ftunivgent https://doi.org/10.1007/s10681-017-2010-5 2023-05-10T22:44:10Z A set of putative marker genes to study plant defense responses against Polyphagotarsonemus latus, a key pest in the production of Rhododendron simsii hybrids, was selected and validated. Genes belonged to the biosynthetic pathway of phytohormones jasmonic acid (JA) (RsLOX, RsAOS, RsAOC, RsOPR3 and RsJMT) and salicylic acid (SA) (RsPAL and RsICS). Furthermore, RsPPO, a putative marker gene for oxidative stress response was successfully cloned from R. simsii. A CTAB-based extraction protocol was optimized to assure excellent RNA quality for subsequent RT-qPCR analysis. The RT-qPCR protocol was extensively tested and RsRG7 and RsRG14 were selected as reference genes from a geNorm pilot study. Validation of the marker genes was done after application with elicitors [methyl jasmonate (MeJA), coronatine, beta-aminobutyric acid and acibenzolar-Smethyl] or wounding. Both 100 mu M MeJA and 0.1 lM coronatine had a significant effect on the expression of all marker genes. Foliar application of MeJA on the shoots resulted in a significantly earlier response when compared to root application and subsequent sampling of the shoots. Expression patterns after MeJA treatment were generally the same in six R. simsii genotypes: 'Nordlicht', 'Elien', 'Aiko Pink', ' Michelle Marie', 'Mevrouw Gerard Kint' and 'Sachsenstern'. Wounding resulted in the same expression patterns as MeJA treatment except for RsJMT. None of the genotypes showed a significant induction of the latter gene 6 h upon wounding. Findings of these experiments indicated that the tolerant genotype 'Elien' has low basal expression levels of RsPPO. This might be the first step towards the breeding of mite-tolerant genotypes. Article in Journal/Newspaper Mite Nordlicht Ghent University Academic Bibliography Euphytica 213 10 |
institution |
Open Polar |
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Ghent University Academic Bibliography |
op_collection_id |
ftunivgent |
language |
English |
topic |
Biology and Life Sciences Jasmonic acid (JA) Methyl jasmonate (MeJA) Polyphagotarsonemus latus Rhododendron simsii hybrids RT-qPCR RNA extraction quality Salicylic acid (SA) LATUS ACARI TARSONEMIDAE TIME RT-PCR ACID CARBOXYL METHYLTRANSFERASE LEAF POLYPHENOL OXIDASE HOT BORATE METHOD HIGH-QUALITY RNA JASMONIC-ACID BROAD MITE POLYPHAGOTARSONEMUS-LATUS ARABIDOPSIS-THALIANA |
spellingShingle |
Biology and Life Sciences Jasmonic acid (JA) Methyl jasmonate (MeJA) Polyphagotarsonemus latus Rhododendron simsii hybrids RT-qPCR RNA extraction quality Salicylic acid (SA) LATUS ACARI TARSONEMIDAE TIME RT-PCR ACID CARBOXYL METHYLTRANSFERASE LEAF POLYPHENOL OXIDASE HOT BORATE METHOD HIGH-QUALITY RNA JASMONIC-ACID BROAD MITE POLYPHAGOTARSONEMUS-LATUS ARABIDOPSIS-THALIANA Luypaert, Gil Witters, Johan Van Huylenbroeck, Johan De Clercq, Patrick De Riek, Jan De Keyser, Ellen Induced expression of selected plant defence related genes in pot azalea, Rhododendron simsii hybrid |
topic_facet |
Biology and Life Sciences Jasmonic acid (JA) Methyl jasmonate (MeJA) Polyphagotarsonemus latus Rhododendron simsii hybrids RT-qPCR RNA extraction quality Salicylic acid (SA) LATUS ACARI TARSONEMIDAE TIME RT-PCR ACID CARBOXYL METHYLTRANSFERASE LEAF POLYPHENOL OXIDASE HOT BORATE METHOD HIGH-QUALITY RNA JASMONIC-ACID BROAD MITE POLYPHAGOTARSONEMUS-LATUS ARABIDOPSIS-THALIANA |
description |
A set of putative marker genes to study plant defense responses against Polyphagotarsonemus latus, a key pest in the production of Rhododendron simsii hybrids, was selected and validated. Genes belonged to the biosynthetic pathway of phytohormones jasmonic acid (JA) (RsLOX, RsAOS, RsAOC, RsOPR3 and RsJMT) and salicylic acid (SA) (RsPAL and RsICS). Furthermore, RsPPO, a putative marker gene for oxidative stress response was successfully cloned from R. simsii. A CTAB-based extraction protocol was optimized to assure excellent RNA quality for subsequent RT-qPCR analysis. The RT-qPCR protocol was extensively tested and RsRG7 and RsRG14 were selected as reference genes from a geNorm pilot study. Validation of the marker genes was done after application with elicitors [methyl jasmonate (MeJA), coronatine, beta-aminobutyric acid and acibenzolar-Smethyl] or wounding. Both 100 mu M MeJA and 0.1 lM coronatine had a significant effect on the expression of all marker genes. Foliar application of MeJA on the shoots resulted in a significantly earlier response when compared to root application and subsequent sampling of the shoots. Expression patterns after MeJA treatment were generally the same in six R. simsii genotypes: 'Nordlicht', 'Elien', 'Aiko Pink', ' Michelle Marie', 'Mevrouw Gerard Kint' and 'Sachsenstern'. Wounding resulted in the same expression patterns as MeJA treatment except for RsJMT. None of the genotypes showed a significant induction of the latter gene 6 h upon wounding. Findings of these experiments indicated that the tolerant genotype 'Elien' has low basal expression levels of RsPPO. This might be the first step towards the breeding of mite-tolerant genotypes. |
format |
Article in Journal/Newspaper |
author |
Luypaert, Gil Witters, Johan Van Huylenbroeck, Johan De Clercq, Patrick De Riek, Jan De Keyser, Ellen |
author_facet |
Luypaert, Gil Witters, Johan Van Huylenbroeck, Johan De Clercq, Patrick De Riek, Jan De Keyser, Ellen |
author_sort |
Luypaert, Gil |
title |
Induced expression of selected plant defence related genes in pot azalea, Rhododendron simsii hybrid |
title_short |
Induced expression of selected plant defence related genes in pot azalea, Rhododendron simsii hybrid |
title_full |
Induced expression of selected plant defence related genes in pot azalea, Rhododendron simsii hybrid |
title_fullStr |
Induced expression of selected plant defence related genes in pot azalea, Rhododendron simsii hybrid |
title_full_unstemmed |
Induced expression of selected plant defence related genes in pot azalea, Rhododendron simsii hybrid |
title_sort |
induced expression of selected plant defence related genes in pot azalea, rhododendron simsii hybrid |
publishDate |
2017 |
url |
https://biblio.ugent.be/publication/8533328 http://hdl.handle.net/1854/LU-8533328 https://doi.org/10.1007/s10681-017-2010-5 https://biblio.ugent.be/publication/8533328/file/8533331 |
genre |
Mite Nordlicht |
genre_facet |
Mite Nordlicht |
op_source |
EUPHYTICA ISSN: 0014-2336 ISSN: 1573-5060 |
op_relation |
https://biblio.ugent.be/publication/8533328 http://hdl.handle.net/1854/LU-8533328 http://dx.doi.org/10.1007/s10681-017-2010-5 https://biblio.ugent.be/publication/8533328/file/8533331 |
op_rights |
No license (in copyright) info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1007/s10681-017-2010-5 |
container_title |
Euphytica |
container_volume |
213 |
container_issue |
10 |
_version_ |
1768377028515463168 |