Biogeographic patterns in Antarctic lacustrine prokaryotes
Amplified climate change, increased human activity and the introduction of alien species likely form the biggest threat to Antarctic terrestrial ecosystems through range size expansions and contractions, regional extirpation and impacts on ecosystem functions. Despite their crucial role in the funct...
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ftunivgent:oai:archive.ugent.be:6934889 2023-06-11T04:06:05+02:00 Biogeographic patterns in Antarctic lacustrine prokaryotes Tytgat, Bjorn Sweetlove, Maxime Verleyen, Elie D'hondt, Sofie Wilmotte, Annick Vyverman, Wim Willems, Anne 2015 https://biblio.ugent.be/publication/6934889 http://hdl.handle.net/1854/LU-6934889 eng eng https://biblio.ugent.be/publication/6934889 http://hdl.handle.net/1854/LU-6934889 Polar and Alpine Microbiology, 6th International conference, Abstracts Biology and Life Sciences ILLUMINA BIOGEOGRAPHY ANTARCTICA CONSERVATION REGIONS PROKARYOTES conference info:eu-repo/semantics/conferenceObject info:eu-repo/semantics/publishedVersion 2015 ftunivgent 2023-05-10T22:30:52Z Amplified climate change, increased human activity and the introduction of alien species likely form the biggest threat to Antarctic terrestrial ecosystems through range size expansions and contractions, regional extirpation and impacts on ecosystem functions. Despite their crucial role in the functioning of Antarctic terrestrial ecosystems, little is known about the present-day diversity and biogeography of microorganisms such as prokaryotes and microeukaryotes in the Antarctic Biogeographic Realm. Furthermore, identification of the key processes underlying microbial biodiversity dynamics is essential to understand and predict the consequences of global change on Antarctic lacustrine ecosystems. We analysed bacterial biodiversity in a total of 152 lacustrine microbial mat samples, distributed over the three main Biogeographic regions in the Antarctic Realm, including continental Antarctica, Maritime Antarctica and the Sub-Antarctic Islands comprising the southern Indian Ocean Province (SIOP) and the southern Pacific Ocean Province (SPOP). We targeted the V1-V3 variable regions of the 16S rRNA gene. Amplicon sequencing was done on an Illumina PE300 MiSeq. Sequences were processed using Usearch and Uparse, Mothur and custom scripts for basic parsing. An OTU cut-off was defined at 97 % sequence similarity, and sequences were mapped against a local GreenGenes database. Downstream analyses were performed using several R packages. We obtained about three million high quality sequences, with an average length of 500 bp. Sequences belonged to 8237 OTUs, and were distributed over 51 phyla and 366 genera. In addition, 649 OTUs remained unclassified at the phylum level and 6263 at the genus level. Mean OTU richness differed strongly between the four biogeographic regions. The lakes from Maritime Antarctica had a higher richness than those from Continental Antarctica. Interestingly, in sub-Antarctica OTU richness was strongly variable, with Marion Island (SIOP) having the lowest and Macquarie Island (SPOP) having on ... Conference Object Antarc* Antarctic Antarctica Macquarie Island Marion Island Ghent University Academic Bibliography Antarctic The Antarctic Pacific Indian |
institution |
Open Polar |
collection |
Ghent University Academic Bibliography |
op_collection_id |
ftunivgent |
language |
English |
topic |
Biology and Life Sciences ILLUMINA BIOGEOGRAPHY ANTARCTICA CONSERVATION REGIONS PROKARYOTES |
spellingShingle |
Biology and Life Sciences ILLUMINA BIOGEOGRAPHY ANTARCTICA CONSERVATION REGIONS PROKARYOTES Tytgat, Bjorn Sweetlove, Maxime Verleyen, Elie D'hondt, Sofie Wilmotte, Annick Vyverman, Wim Willems, Anne Biogeographic patterns in Antarctic lacustrine prokaryotes |
topic_facet |
Biology and Life Sciences ILLUMINA BIOGEOGRAPHY ANTARCTICA CONSERVATION REGIONS PROKARYOTES |
description |
Amplified climate change, increased human activity and the introduction of alien species likely form the biggest threat to Antarctic terrestrial ecosystems through range size expansions and contractions, regional extirpation and impacts on ecosystem functions. Despite their crucial role in the functioning of Antarctic terrestrial ecosystems, little is known about the present-day diversity and biogeography of microorganisms such as prokaryotes and microeukaryotes in the Antarctic Biogeographic Realm. Furthermore, identification of the key processes underlying microbial biodiversity dynamics is essential to understand and predict the consequences of global change on Antarctic lacustrine ecosystems. We analysed bacterial biodiversity in a total of 152 lacustrine microbial mat samples, distributed over the three main Biogeographic regions in the Antarctic Realm, including continental Antarctica, Maritime Antarctica and the Sub-Antarctic Islands comprising the southern Indian Ocean Province (SIOP) and the southern Pacific Ocean Province (SPOP). We targeted the V1-V3 variable regions of the 16S rRNA gene. Amplicon sequencing was done on an Illumina PE300 MiSeq. Sequences were processed using Usearch and Uparse, Mothur and custom scripts for basic parsing. An OTU cut-off was defined at 97 % sequence similarity, and sequences were mapped against a local GreenGenes database. Downstream analyses were performed using several R packages. We obtained about three million high quality sequences, with an average length of 500 bp. Sequences belonged to 8237 OTUs, and were distributed over 51 phyla and 366 genera. In addition, 649 OTUs remained unclassified at the phylum level and 6263 at the genus level. Mean OTU richness differed strongly between the four biogeographic regions. The lakes from Maritime Antarctica had a higher richness than those from Continental Antarctica. Interestingly, in sub-Antarctica OTU richness was strongly variable, with Marion Island (SIOP) having the lowest and Macquarie Island (SPOP) having on ... |
format |
Conference Object |
author |
Tytgat, Bjorn Sweetlove, Maxime Verleyen, Elie D'hondt, Sofie Wilmotte, Annick Vyverman, Wim Willems, Anne |
author_facet |
Tytgat, Bjorn Sweetlove, Maxime Verleyen, Elie D'hondt, Sofie Wilmotte, Annick Vyverman, Wim Willems, Anne |
author_sort |
Tytgat, Bjorn |
title |
Biogeographic patterns in Antarctic lacustrine prokaryotes |
title_short |
Biogeographic patterns in Antarctic lacustrine prokaryotes |
title_full |
Biogeographic patterns in Antarctic lacustrine prokaryotes |
title_fullStr |
Biogeographic patterns in Antarctic lacustrine prokaryotes |
title_full_unstemmed |
Biogeographic patterns in Antarctic lacustrine prokaryotes |
title_sort |
biogeographic patterns in antarctic lacustrine prokaryotes |
publishDate |
2015 |
url |
https://biblio.ugent.be/publication/6934889 http://hdl.handle.net/1854/LU-6934889 |
geographic |
Antarctic The Antarctic Pacific Indian |
geographic_facet |
Antarctic The Antarctic Pacific Indian |
genre |
Antarc* Antarctic Antarctica Macquarie Island Marion Island |
genre_facet |
Antarc* Antarctic Antarctica Macquarie Island Marion Island |
op_source |
Polar and Alpine Microbiology, 6th International conference, Abstracts |
op_relation |
https://biblio.ugent.be/publication/6934889 http://hdl.handle.net/1854/LU-6934889 |
_version_ |
1768377847044374528 |