Diversity of CBBL, NIFH and PUFLM genes in soils around the Princess Elisabeth station, Sør Rondane Mountains, Antarctica
Introduction: In Antarctica, the presence of native flowering plants is restricted to the Peninsula. In continental Antarctica, photosynthesis by Cyanobacteria is generally thought to be the main primary source of organic carbon. Many cyanobacterial species are also capable of fixing nitrogen, allow...
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ftunivgent:oai:archive.ugent.be:6934848 2023-06-11T04:05:31+02:00 Diversity of CBBL, NIFH and PUFLM genes in soils around the Princess Elisabeth station, Sør Rondane Mountains, Antarctica Tahon, Guillaume Tytgat, Bjorn Willems, Anne 2015 https://biblio.ugent.be/publication/6934848 http://hdl.handle.net/1854/LU-6934848 eng eng https://biblio.ugent.be/publication/6934848 http://hdl.handle.net/1854/LU-6934848 Polar and Alpine Microbiology, 6th International conference, Abstracts Biology and Life Sciences ANTARCTICA ILLUMINA CARBON FIXATION NITROGEN FIXATION conference info:eu-repo/semantics/conferenceObject info:eu-repo/semantics/publishedVersion 2015 ftunivgent 2023-05-10T22:30:52Z Introduction: In Antarctica, the presence of native flowering plants is restricted to the Peninsula. In continental Antarctica, photosynthesis by Cyanobacteria is generally thought to be the main primary source of organic carbon. Many cyanobacterial species are also capable of fixing nitrogen, allowing them to survive and prosper in almost every environment, including the adverse conditions of Antarctica. Several studies, including our own findings, however, have shown that Cyanobacteria are sometimes only scarcely present in the terrestrial Antarctic microbial communities. Furthermore, both carbon and nitrogen fixation require a considerable amount of ATP. In Antarctica, sunlight – an abundant energy source during the Antarctic summer – may represent an important resource to generate this ATP. Some bacteria, for example, are known to use rhodopsin-like pigments to exploit sunlight, whereas aerobic anoxygenic photosynthetic bacteria can use bacteriochlorophyll to harvest light energy that is then stored as ATP. We explored the hypothesis that, in the ice-free regions of continental Antarctica, other primary producers and bacteria that exploit solar energy may contribute to carbon and nitrogen fixation, in addition to Cyanobacteria. Methods & Materials: The presence and diversity of non-cyanobacterial prokaryotes that possess these properties was studied in four terrestrial samples gathered in the vicinity of the Belgian Princess Elisabeth Station (Sør Rondane Mountains, Queen Maud Land, East Antarctica). A culture independent approach by Illumina MiSeq amplicon sequencing of cbbL (carbon fixation, RuBisCO type I), nifH (nitrogen fixation), and pufLM and proteorhodopsin genes (light-harvesting) was used. After curation, sequences were placed in phylogenies with existing sequence data to reveal phylogenetic affiliations. Results: Proteorhodopsin genes failed to amplify from all tested samples. Illumina sequencing extended the functional genes’ sequence datasets, previously obtained by performing PCR clone ... Conference Object Antarc* Antarctic Antarctica East Antarctica Queen Maud Land Ghent University Academic Bibliography Antarctic The Antarctic East Antarctica Queen Maud Land ENVELOPE(12.000,12.000,-72.500,-72.500) Sør-Rondane ENVELOPE(25.000,25.000,-72.000,-72.000) Sør Rondane Mountains ENVELOPE(25.000,25.000,-72.000,-72.000) |
institution |
Open Polar |
collection |
Ghent University Academic Bibliography |
op_collection_id |
ftunivgent |
language |
English |
topic |
Biology and Life Sciences ANTARCTICA ILLUMINA CARBON FIXATION NITROGEN FIXATION |
spellingShingle |
Biology and Life Sciences ANTARCTICA ILLUMINA CARBON FIXATION NITROGEN FIXATION Tahon, Guillaume Tytgat, Bjorn Willems, Anne Diversity of CBBL, NIFH and PUFLM genes in soils around the Princess Elisabeth station, Sør Rondane Mountains, Antarctica |
topic_facet |
Biology and Life Sciences ANTARCTICA ILLUMINA CARBON FIXATION NITROGEN FIXATION |
description |
Introduction: In Antarctica, the presence of native flowering plants is restricted to the Peninsula. In continental Antarctica, photosynthesis by Cyanobacteria is generally thought to be the main primary source of organic carbon. Many cyanobacterial species are also capable of fixing nitrogen, allowing them to survive and prosper in almost every environment, including the adverse conditions of Antarctica. Several studies, including our own findings, however, have shown that Cyanobacteria are sometimes only scarcely present in the terrestrial Antarctic microbial communities. Furthermore, both carbon and nitrogen fixation require a considerable amount of ATP. In Antarctica, sunlight – an abundant energy source during the Antarctic summer – may represent an important resource to generate this ATP. Some bacteria, for example, are known to use rhodopsin-like pigments to exploit sunlight, whereas aerobic anoxygenic photosynthetic bacteria can use bacteriochlorophyll to harvest light energy that is then stored as ATP. We explored the hypothesis that, in the ice-free regions of continental Antarctica, other primary producers and bacteria that exploit solar energy may contribute to carbon and nitrogen fixation, in addition to Cyanobacteria. Methods & Materials: The presence and diversity of non-cyanobacterial prokaryotes that possess these properties was studied in four terrestrial samples gathered in the vicinity of the Belgian Princess Elisabeth Station (Sør Rondane Mountains, Queen Maud Land, East Antarctica). A culture independent approach by Illumina MiSeq amplicon sequencing of cbbL (carbon fixation, RuBisCO type I), nifH (nitrogen fixation), and pufLM and proteorhodopsin genes (light-harvesting) was used. After curation, sequences were placed in phylogenies with existing sequence data to reveal phylogenetic affiliations. Results: Proteorhodopsin genes failed to amplify from all tested samples. Illumina sequencing extended the functional genes’ sequence datasets, previously obtained by performing PCR clone ... |
format |
Conference Object |
author |
Tahon, Guillaume Tytgat, Bjorn Willems, Anne |
author_facet |
Tahon, Guillaume Tytgat, Bjorn Willems, Anne |
author_sort |
Tahon, Guillaume |
title |
Diversity of CBBL, NIFH and PUFLM genes in soils around the Princess Elisabeth station, Sør Rondane Mountains, Antarctica |
title_short |
Diversity of CBBL, NIFH and PUFLM genes in soils around the Princess Elisabeth station, Sør Rondane Mountains, Antarctica |
title_full |
Diversity of CBBL, NIFH and PUFLM genes in soils around the Princess Elisabeth station, Sør Rondane Mountains, Antarctica |
title_fullStr |
Diversity of CBBL, NIFH and PUFLM genes in soils around the Princess Elisabeth station, Sør Rondane Mountains, Antarctica |
title_full_unstemmed |
Diversity of CBBL, NIFH and PUFLM genes in soils around the Princess Elisabeth station, Sør Rondane Mountains, Antarctica |
title_sort |
diversity of cbbl, nifh and puflm genes in soils around the princess elisabeth station, sør rondane mountains, antarctica |
publishDate |
2015 |
url |
https://biblio.ugent.be/publication/6934848 http://hdl.handle.net/1854/LU-6934848 |
long_lat |
ENVELOPE(12.000,12.000,-72.500,-72.500) ENVELOPE(25.000,25.000,-72.000,-72.000) ENVELOPE(25.000,25.000,-72.000,-72.000) |
geographic |
Antarctic The Antarctic East Antarctica Queen Maud Land Sør-Rondane Sør Rondane Mountains |
geographic_facet |
Antarctic The Antarctic East Antarctica Queen Maud Land Sør-Rondane Sør Rondane Mountains |
genre |
Antarc* Antarctic Antarctica East Antarctica Queen Maud Land |
genre_facet |
Antarc* Antarctic Antarctica East Antarctica Queen Maud Land |
op_source |
Polar and Alpine Microbiology, 6th International conference, Abstracts |
op_relation |
https://biblio.ugent.be/publication/6934848 http://hdl.handle.net/1854/LU-6934848 |
_version_ |
1768376789959180288 |