Comparison of diagnostic tools for the detection of aspergillosis in blood samples of experimentally infected falcons
Antemortem diagnosis of avian aspergillosis is very challenging. Diagnostic assays using blood samples would aid in an early and more definitive diagnosis. In the current study, detection of anti-Aspergillus antibodies, Aspergillus antigen, and Aspergillus toxin (fumigaclavine A), protein electropho...
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2014
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Online Access: | https://biblio.ugent.be/publication/5947818 http://hdl.handle.net/1854/LU-5947818 https://doi.org/10.1637/10831-032714-Reg https://biblio.ugent.be/publication/5947818/file/5947869 |
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ftunivgent:oai:archive.ugent.be:5947818 2023-06-11T04:11:35+02:00 Comparison of diagnostic tools for the detection of aspergillosis in blood samples of experimentally infected falcons Fischer, D Van Waeyenberghe, Lieven Cray, C Gross, M Usleber, E Pasmans, Frank Martel, An Lierz, M 2014 application/pdf https://biblio.ugent.be/publication/5947818 http://hdl.handle.net/1854/LU-5947818 https://doi.org/10.1637/10831-032714-Reg https://biblio.ugent.be/publication/5947818/file/5947869 eng eng https://biblio.ugent.be/publication/5947818 http://hdl.handle.net/1854/LU-5947818 http://dx.doi.org/10.1637/10831-032714-Reg https://biblio.ugent.be/publication/5947818/file/5947869 No license (in copyright) info:eu-repo/semantics/restrictedAccess AVIAN DISEASES ISSN: 0005-2086 Veterinary Sciences DISEASE FUMIGATUS PLASMA MARKERS GALACTOMANNAN ELECTROPHORESIS PSITTACINE BIRDS INVASIVE ASPERGILLOSIS LINKED-IMMUNOSORBENT-ASSAY ACUTE-PHASE PROTEINS haptoglobin birds of prey anti-Aspergillus antibody fumigaclavine A raptors acute-phase protein serologic test protein electrophoresis Aspergillus fumigatus journalArticle info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2014 ftunivgent https://doi.org/10.1637/10831-032714-Reg 2023-05-10T22:42:42Z Antemortem diagnosis of avian aspergillosis is very challenging. Diagnostic assays using blood samples would aid in an early and more definitive diagnosis. In the current study, detection of anti-Aspergillus antibodies, Aspergillus antigen, and Aspergillus toxin (fumigaclavine A), protein electrophoresis and measurement of acute-phase protein concentrations were performed on serum of 18 adult and plasma of 21 juvenile gyr-saker hybrid falcons (Falco rusticolus x Falco cherrug). Adult (n = 15) and juvenile (n = 18) falcons were experimentally inoculated with different dosages of the same strain of Aspergillus fumigatus and an additional three falcons from each age group were used as uninfected control animals. Blood samples were collected prior to inoculation and at 28 days postinoculation. Of the 33 inoculated falcons, 16 demonstrated clinical signs (vomiting, greenish urates, dyspnea, ruffled feathers) commonly associated with aspergillosis and in 14 falcons necropsy revealed aspergillosis granulomas confirmed by mycology and histopathology. Positive galactomannan results were rare, with only 3/15 positive samples from adult falcons and none in the juvenile birds. Most of the inoculated falcons showed an increase of serum amyloid A (66.7%) and haptoglobin (70.4%), but fumigaclavine A was not detected in the blood from any of the experimental animals. Elevated antibody indices were detected in 96.7% of the inoculated birds, but also in 66.7% of the controls. Significant decreases in albumin: globulin ratio were obvious in 81.5% of the inoculated birds, including 100% of the birds with granulomas. Blood from falcons with granulomas demonstrated significantly increased concentration values of alpha 2 and beta globulins, decreased percentages of prealbumin and albumin, and increased percentages of alpha 2 and beta globulins compared to inoculated falcons without granulomas. In conclusion, acute-phase proteins and the electrophoretic profile of birds challenged with A. fumigatus show significant alterations, which ... Article in Journal/Newspaper Falco rusticolus Ghent University Academic Bibliography Avian Diseases 58 4 587 598 |
institution |
Open Polar |
collection |
Ghent University Academic Bibliography |
op_collection_id |
ftunivgent |
language |
English |
topic |
Veterinary Sciences DISEASE FUMIGATUS PLASMA MARKERS GALACTOMANNAN ELECTROPHORESIS PSITTACINE BIRDS INVASIVE ASPERGILLOSIS LINKED-IMMUNOSORBENT-ASSAY ACUTE-PHASE PROTEINS haptoglobin birds of prey anti-Aspergillus antibody fumigaclavine A raptors acute-phase protein serologic test protein electrophoresis Aspergillus fumigatus |
spellingShingle |
Veterinary Sciences DISEASE FUMIGATUS PLASMA MARKERS GALACTOMANNAN ELECTROPHORESIS PSITTACINE BIRDS INVASIVE ASPERGILLOSIS LINKED-IMMUNOSORBENT-ASSAY ACUTE-PHASE PROTEINS haptoglobin birds of prey anti-Aspergillus antibody fumigaclavine A raptors acute-phase protein serologic test protein electrophoresis Aspergillus fumigatus Fischer, D Van Waeyenberghe, Lieven Cray, C Gross, M Usleber, E Pasmans, Frank Martel, An Lierz, M Comparison of diagnostic tools for the detection of aspergillosis in blood samples of experimentally infected falcons |
topic_facet |
Veterinary Sciences DISEASE FUMIGATUS PLASMA MARKERS GALACTOMANNAN ELECTROPHORESIS PSITTACINE BIRDS INVASIVE ASPERGILLOSIS LINKED-IMMUNOSORBENT-ASSAY ACUTE-PHASE PROTEINS haptoglobin birds of prey anti-Aspergillus antibody fumigaclavine A raptors acute-phase protein serologic test protein electrophoresis Aspergillus fumigatus |
description |
Antemortem diagnosis of avian aspergillosis is very challenging. Diagnostic assays using blood samples would aid in an early and more definitive diagnosis. In the current study, detection of anti-Aspergillus antibodies, Aspergillus antigen, and Aspergillus toxin (fumigaclavine A), protein electrophoresis and measurement of acute-phase protein concentrations were performed on serum of 18 adult and plasma of 21 juvenile gyr-saker hybrid falcons (Falco rusticolus x Falco cherrug). Adult (n = 15) and juvenile (n = 18) falcons were experimentally inoculated with different dosages of the same strain of Aspergillus fumigatus and an additional three falcons from each age group were used as uninfected control animals. Blood samples were collected prior to inoculation and at 28 days postinoculation. Of the 33 inoculated falcons, 16 demonstrated clinical signs (vomiting, greenish urates, dyspnea, ruffled feathers) commonly associated with aspergillosis and in 14 falcons necropsy revealed aspergillosis granulomas confirmed by mycology and histopathology. Positive galactomannan results were rare, with only 3/15 positive samples from adult falcons and none in the juvenile birds. Most of the inoculated falcons showed an increase of serum amyloid A (66.7%) and haptoglobin (70.4%), but fumigaclavine A was not detected in the blood from any of the experimental animals. Elevated antibody indices were detected in 96.7% of the inoculated birds, but also in 66.7% of the controls. Significant decreases in albumin: globulin ratio were obvious in 81.5% of the inoculated birds, including 100% of the birds with granulomas. Blood from falcons with granulomas demonstrated significantly increased concentration values of alpha 2 and beta globulins, decreased percentages of prealbumin and albumin, and increased percentages of alpha 2 and beta globulins compared to inoculated falcons without granulomas. In conclusion, acute-phase proteins and the electrophoretic profile of birds challenged with A. fumigatus show significant alterations, which ... |
format |
Article in Journal/Newspaper |
author |
Fischer, D Van Waeyenberghe, Lieven Cray, C Gross, M Usleber, E Pasmans, Frank Martel, An Lierz, M |
author_facet |
Fischer, D Van Waeyenberghe, Lieven Cray, C Gross, M Usleber, E Pasmans, Frank Martel, An Lierz, M |
author_sort |
Fischer, D |
title |
Comparison of diagnostic tools for the detection of aspergillosis in blood samples of experimentally infected falcons |
title_short |
Comparison of diagnostic tools for the detection of aspergillosis in blood samples of experimentally infected falcons |
title_full |
Comparison of diagnostic tools for the detection of aspergillosis in blood samples of experimentally infected falcons |
title_fullStr |
Comparison of diagnostic tools for the detection of aspergillosis in blood samples of experimentally infected falcons |
title_full_unstemmed |
Comparison of diagnostic tools for the detection of aspergillosis in blood samples of experimentally infected falcons |
title_sort |
comparison of diagnostic tools for the detection of aspergillosis in blood samples of experimentally infected falcons |
publishDate |
2014 |
url |
https://biblio.ugent.be/publication/5947818 http://hdl.handle.net/1854/LU-5947818 https://doi.org/10.1637/10831-032714-Reg https://biblio.ugent.be/publication/5947818/file/5947869 |
genre |
Falco rusticolus |
genre_facet |
Falco rusticolus |
op_source |
AVIAN DISEASES ISSN: 0005-2086 |
op_relation |
https://biblio.ugent.be/publication/5947818 http://hdl.handle.net/1854/LU-5947818 http://dx.doi.org/10.1637/10831-032714-Reg https://biblio.ugent.be/publication/5947818/file/5947869 |
op_rights |
No license (in copyright) info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1637/10831-032714-Reg |
container_title |
Avian Diseases |
container_volume |
58 |
container_issue |
4 |
container_start_page |
587 |
op_container_end_page |
598 |
_version_ |
1768386756055400448 |