Microsatellite conservation and Bayesian individual assignment in four Anguilla species
Microsatellite flanking regions are often highly conserved in fish taxa, enabling their application in other species within or outside the source family. Moderately variable microsatellite markers may also be good candidates for species identification using multi-locus genotypes. We evaluated the de...
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ftunivgent:oai:archive.ugent.be:5697837 2023-06-11T04:03:45+02:00 Microsatellite conservation and Bayesian individual assignment in four Anguilla species Maes, GE Pujolar, JM Raeymaekers, Joost Dannewitz, J Volckaert, FAM 2006 application/pdf https://biblio.ugent.be/publication/5697837 http://hdl.handle.net/1854/LU-5697837 https://doi.org/10.3354/meps319251 https://biblio.ugent.be/publication/5697837/file/5700386 eng eng https://biblio.ugent.be/publication/5697837 http://hdl.handle.net/1854/LU-5697837 http://dx.doi.org/10.3354/meps319251 https://biblio.ugent.be/publication/5697837/file/5700386 No license (in copyright) info:eu-repo/semantics/openAccess MARINE ECOLOGY PROGRESS SERIES ISSN: 0171-8630 Biology and Life Sciences DIFFERENTIATION FRESH-WATER EELS POLYMORPHISM DNA LOCI GENUS ANGUILLA GENETIC-EVIDENCE POPULATION-STRUCTURE EUROPEAN EEL POLYMERASE-CHAIN-REACTION species identification multiplex-PCR homoplasy genetic variability anguillids admixture flanking region journalArticle info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2006 ftunivgent https://doi.org/10.3354/meps319251 2023-04-19T22:09:37Z Microsatellite flanking regions are often highly conserved in fish taxa, enabling their application in other species within or outside the source family. Moderately variable microsatellite markers may also be good candidates for species identification using multi-locus genotypes. We evaluated the degree of conservation of microsatellite flanking regions and the level of polymorphism in 4 commercially important eel species (Anguilla anguilla, A. rostrata, A. japonica and A, marmorata). Using multiplex polymerase chain reactions developed for the first 2 taxa, we assessed the discrimination power of an individual-based assignment method to differentiate all 4 species without initial species information. Detection and classification of each species was performed with high confidence (> 90%), as was assignment of randomly sampled individuals to pre-defined species (> 95%). Our results demonstrate the highly conserved nature of microsatellites and their level of polymorphism in Anguilla species. Although an inverse relationship was found between genetic diversity and differentiation estimates, likely due to homoplasy, assignment proved to be superior to multivariate and distance-based approaches for identifying the 4 species. The method enables the rapid screening of morphologically similar eel species using only 4 co-dominant nuclear loci and the detection of natural hybridisation or anthropogenic mixing between internationally highly traded species. Article in Journal/Newspaper Anguilla anguilla European eel Ghent University Academic Bibliography Marine Ecology Progress Series 319 251 261 |
institution |
Open Polar |
collection |
Ghent University Academic Bibliography |
op_collection_id |
ftunivgent |
language |
English |
topic |
Biology and Life Sciences DIFFERENTIATION FRESH-WATER EELS POLYMORPHISM DNA LOCI GENUS ANGUILLA GENETIC-EVIDENCE POPULATION-STRUCTURE EUROPEAN EEL POLYMERASE-CHAIN-REACTION species identification multiplex-PCR homoplasy genetic variability anguillids admixture flanking region |
spellingShingle |
Biology and Life Sciences DIFFERENTIATION FRESH-WATER EELS POLYMORPHISM DNA LOCI GENUS ANGUILLA GENETIC-EVIDENCE POPULATION-STRUCTURE EUROPEAN EEL POLYMERASE-CHAIN-REACTION species identification multiplex-PCR homoplasy genetic variability anguillids admixture flanking region Maes, GE Pujolar, JM Raeymaekers, Joost Dannewitz, J Volckaert, FAM Microsatellite conservation and Bayesian individual assignment in four Anguilla species |
topic_facet |
Biology and Life Sciences DIFFERENTIATION FRESH-WATER EELS POLYMORPHISM DNA LOCI GENUS ANGUILLA GENETIC-EVIDENCE POPULATION-STRUCTURE EUROPEAN EEL POLYMERASE-CHAIN-REACTION species identification multiplex-PCR homoplasy genetic variability anguillids admixture flanking region |
description |
Microsatellite flanking regions are often highly conserved in fish taxa, enabling their application in other species within or outside the source family. Moderately variable microsatellite markers may also be good candidates for species identification using multi-locus genotypes. We evaluated the degree of conservation of microsatellite flanking regions and the level of polymorphism in 4 commercially important eel species (Anguilla anguilla, A. rostrata, A. japonica and A, marmorata). Using multiplex polymerase chain reactions developed for the first 2 taxa, we assessed the discrimination power of an individual-based assignment method to differentiate all 4 species without initial species information. Detection and classification of each species was performed with high confidence (> 90%), as was assignment of randomly sampled individuals to pre-defined species (> 95%). Our results demonstrate the highly conserved nature of microsatellites and their level of polymorphism in Anguilla species. Although an inverse relationship was found between genetic diversity and differentiation estimates, likely due to homoplasy, assignment proved to be superior to multivariate and distance-based approaches for identifying the 4 species. The method enables the rapid screening of morphologically similar eel species using only 4 co-dominant nuclear loci and the detection of natural hybridisation or anthropogenic mixing between internationally highly traded species. |
format |
Article in Journal/Newspaper |
author |
Maes, GE Pujolar, JM Raeymaekers, Joost Dannewitz, J Volckaert, FAM |
author_facet |
Maes, GE Pujolar, JM Raeymaekers, Joost Dannewitz, J Volckaert, FAM |
author_sort |
Maes, GE |
title |
Microsatellite conservation and Bayesian individual assignment in four Anguilla species |
title_short |
Microsatellite conservation and Bayesian individual assignment in four Anguilla species |
title_full |
Microsatellite conservation and Bayesian individual assignment in four Anguilla species |
title_fullStr |
Microsatellite conservation and Bayesian individual assignment in four Anguilla species |
title_full_unstemmed |
Microsatellite conservation and Bayesian individual assignment in four Anguilla species |
title_sort |
microsatellite conservation and bayesian individual assignment in four anguilla species |
publishDate |
2006 |
url |
https://biblio.ugent.be/publication/5697837 http://hdl.handle.net/1854/LU-5697837 https://doi.org/10.3354/meps319251 https://biblio.ugent.be/publication/5697837/file/5700386 |
genre |
Anguilla anguilla European eel |
genre_facet |
Anguilla anguilla European eel |
op_source |
MARINE ECOLOGY PROGRESS SERIES ISSN: 0171-8630 |
op_relation |
https://biblio.ugent.be/publication/5697837 http://hdl.handle.net/1854/LU-5697837 http://dx.doi.org/10.3354/meps319251 https://biblio.ugent.be/publication/5697837/file/5700386 |
op_rights |
No license (in copyright) info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.3354/meps319251 |
container_title |
Marine Ecology Progress Series |
container_volume |
319 |
container_start_page |
251 |
op_container_end_page |
261 |
_version_ |
1768383013279760384 |