Effects of poly-β-hydroxybutyrate (PHB) on Siberian sturgeon (Acipenser baerii) fingerlings performance and its gastrointestinal tract microbial community
Poly-beta-hydroxybutyrate (PHB) is a natural polymer that can be depolymerized into water-soluble short-chain fatty acid monomers. These monomers can act as microbial control agents. In this study, the effects of partially replacing the diet of Siberian sturgeon fingerlings with 2% and 5% PHB were i...
Published in: | FEMS Microbiology Ecology |
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ftunivgent:oai:archive.ugent.be:2005246 2023-10-01T03:49:39+02:00 Effects of poly-β-hydroxybutyrate (PHB) on Siberian sturgeon (Acipenser baerii) fingerlings performance and its gastrointestinal tract microbial community Hossein Najdegerami, Ebrahim Tiet, Ngoc Tran Defoirdt, Tom Marzorati, Massimo Sorgeloos, Patrick Boon, Nico Bossier, Peter 2012 application/pdf https://biblio.ugent.be/publication/2005246 http://hdl.handle.net/1854/LU-2005246 https://doi.org/10.1111/j.1574-6941.2011.01194.x https://biblio.ugent.be/publication/2005246/file/2005300 eng eng https://biblio.ugent.be/publication/2005246 http://hdl.handle.net/1854/LU-2005246 http://dx.doi.org/10.1111/j.1574-6941.2011.01194.x https://biblio.ugent.be/publication/2005246/file/2005300 No license (in copyright) info:eu-repo/semantics/restrictedAccess FEMS MICROBIOLOGY ECOLOGY ISSN: 0168-6496 Biology and Life Sciences DIETARY SUPPLEMENTATION SUBSTRATE UTILIZATION GROWTH-PERFORMANCE X MORONE-SAXATILIS MANNAN OLIGOSACCHARIDES MOS PATHOGENIC VIBRIO-CAMPBELLII GRADIENT GEL-ELECTROPHORESIS microbial community Siberian sturgeon prebiotics poly-β-hydroxybutyrate ARTEMIA-FRANCISCANA BREWERS-YEAST journalArticle info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2012 ftunivgent https://doi.org/10.1111/j.1574-6941.2011.01194.x 2023-09-06T22:31:03Z Poly-beta-hydroxybutyrate (PHB) is a natural polymer that can be depolymerized into water-soluble short-chain fatty acid monomers. These monomers can act as microbial control agents. In this study, the effects of partially replacing the diet of Siberian sturgeon fingerlings with 2% and 5% PHB were investigated. Replacing 2% of the diet with PHB improved weight gain, specific growth rate (SGR) and survival in the sturgeon fingerlings during the 10-week experimental period. Community-level physiological profiling and PCR-denaturing gradient gel electrophoresis (PCR-DGGE) were used to analyze the microbial community diversity and community organization in the sturgeon gastrointestinal tract. DGGE analysis revealed that PHB affected the intestinal microbial species richness and diversity. The highest species richness was observed with 2% PHB. DNA sequencing of the dominant bands in 2% and 5% PHB treatments revealed that PHB stimulated bacteria belonging to the genera Bacillus and Ruminococcaceae. Principal component analysis, Lorenz curves and the Shannon index of Biolog(TM) Ecoplate data revealed that aerobic metabolic potential of the bacterial community was different in the PHB-treated fishes as compared with the control situation. Overall, our results indicate that PHB act as microbial control agents and replacement of 2% of Siberian sturgeon fingerling diet with PHB has beneficial effects. Article in Journal/Newspaper Acipenser baerii Siberian sturgeon Ghent University Academic Bibliography FEMS Microbiology Ecology 79 1 25 33 |
institution |
Open Polar |
collection |
Ghent University Academic Bibliography |
op_collection_id |
ftunivgent |
language |
English |
topic |
Biology and Life Sciences DIETARY SUPPLEMENTATION SUBSTRATE UTILIZATION GROWTH-PERFORMANCE X MORONE-SAXATILIS MANNAN OLIGOSACCHARIDES MOS PATHOGENIC VIBRIO-CAMPBELLII GRADIENT GEL-ELECTROPHORESIS microbial community Siberian sturgeon prebiotics poly-β-hydroxybutyrate ARTEMIA-FRANCISCANA BREWERS-YEAST |
spellingShingle |
Biology and Life Sciences DIETARY SUPPLEMENTATION SUBSTRATE UTILIZATION GROWTH-PERFORMANCE X MORONE-SAXATILIS MANNAN OLIGOSACCHARIDES MOS PATHOGENIC VIBRIO-CAMPBELLII GRADIENT GEL-ELECTROPHORESIS microbial community Siberian sturgeon prebiotics poly-β-hydroxybutyrate ARTEMIA-FRANCISCANA BREWERS-YEAST Hossein Najdegerami, Ebrahim Tiet, Ngoc Tran Defoirdt, Tom Marzorati, Massimo Sorgeloos, Patrick Boon, Nico Bossier, Peter Effects of poly-β-hydroxybutyrate (PHB) on Siberian sturgeon (Acipenser baerii) fingerlings performance and its gastrointestinal tract microbial community |
topic_facet |
Biology and Life Sciences DIETARY SUPPLEMENTATION SUBSTRATE UTILIZATION GROWTH-PERFORMANCE X MORONE-SAXATILIS MANNAN OLIGOSACCHARIDES MOS PATHOGENIC VIBRIO-CAMPBELLII GRADIENT GEL-ELECTROPHORESIS microbial community Siberian sturgeon prebiotics poly-β-hydroxybutyrate ARTEMIA-FRANCISCANA BREWERS-YEAST |
description |
Poly-beta-hydroxybutyrate (PHB) is a natural polymer that can be depolymerized into water-soluble short-chain fatty acid monomers. These monomers can act as microbial control agents. In this study, the effects of partially replacing the diet of Siberian sturgeon fingerlings with 2% and 5% PHB were investigated. Replacing 2% of the diet with PHB improved weight gain, specific growth rate (SGR) and survival in the sturgeon fingerlings during the 10-week experimental period. Community-level physiological profiling and PCR-denaturing gradient gel electrophoresis (PCR-DGGE) were used to analyze the microbial community diversity and community organization in the sturgeon gastrointestinal tract. DGGE analysis revealed that PHB affected the intestinal microbial species richness and diversity. The highest species richness was observed with 2% PHB. DNA sequencing of the dominant bands in 2% and 5% PHB treatments revealed that PHB stimulated bacteria belonging to the genera Bacillus and Ruminococcaceae. Principal component analysis, Lorenz curves and the Shannon index of Biolog(TM) Ecoplate data revealed that aerobic metabolic potential of the bacterial community was different in the PHB-treated fishes as compared with the control situation. Overall, our results indicate that PHB act as microbial control agents and replacement of 2% of Siberian sturgeon fingerling diet with PHB has beneficial effects. |
format |
Article in Journal/Newspaper |
author |
Hossein Najdegerami, Ebrahim Tiet, Ngoc Tran Defoirdt, Tom Marzorati, Massimo Sorgeloos, Patrick Boon, Nico Bossier, Peter |
author_facet |
Hossein Najdegerami, Ebrahim Tiet, Ngoc Tran Defoirdt, Tom Marzorati, Massimo Sorgeloos, Patrick Boon, Nico Bossier, Peter |
author_sort |
Hossein Najdegerami, Ebrahim |
title |
Effects of poly-β-hydroxybutyrate (PHB) on Siberian sturgeon (Acipenser baerii) fingerlings performance and its gastrointestinal tract microbial community |
title_short |
Effects of poly-β-hydroxybutyrate (PHB) on Siberian sturgeon (Acipenser baerii) fingerlings performance and its gastrointestinal tract microbial community |
title_full |
Effects of poly-β-hydroxybutyrate (PHB) on Siberian sturgeon (Acipenser baerii) fingerlings performance and its gastrointestinal tract microbial community |
title_fullStr |
Effects of poly-β-hydroxybutyrate (PHB) on Siberian sturgeon (Acipenser baerii) fingerlings performance and its gastrointestinal tract microbial community |
title_full_unstemmed |
Effects of poly-β-hydroxybutyrate (PHB) on Siberian sturgeon (Acipenser baerii) fingerlings performance and its gastrointestinal tract microbial community |
title_sort |
effects of poly-β-hydroxybutyrate (phb) on siberian sturgeon (acipenser baerii) fingerlings performance and its gastrointestinal tract microbial community |
publishDate |
2012 |
url |
https://biblio.ugent.be/publication/2005246 http://hdl.handle.net/1854/LU-2005246 https://doi.org/10.1111/j.1574-6941.2011.01194.x https://biblio.ugent.be/publication/2005246/file/2005300 |
genre |
Acipenser baerii Siberian sturgeon |
genre_facet |
Acipenser baerii Siberian sturgeon |
op_source |
FEMS MICROBIOLOGY ECOLOGY ISSN: 0168-6496 |
op_relation |
https://biblio.ugent.be/publication/2005246 http://hdl.handle.net/1854/LU-2005246 http://dx.doi.org/10.1111/j.1574-6941.2011.01194.x https://biblio.ugent.be/publication/2005246/file/2005300 |
op_rights |
No license (in copyright) info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1111/j.1574-6941.2011.01194.x |
container_title |
FEMS Microbiology Ecology |
container_volume |
79 |
container_issue |
1 |
container_start_page |
25 |
op_container_end_page |
33 |
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1778521575025278976 |