Culturable diversity of heterotrophic bacteria in Forlidas Pond (Pensacola Mountains) and Lundström Lake (Shackleton Range), Antarctica
Cultivation techniques were used to study the heterotrophic bacterial diversity in two microbial mat samples originating from the littoral zone of two continental Antarctic lakes (Forlidas Pond and Lundström Lake) in the Dufek Massif (within the Pensacola Mountains group of the Transantarctic Mounta...
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ftunivgent:oai:archive.ugent.be:1220529 2023-10-01T03:51:12+02:00 Culturable diversity of heterotrophic bacteria in Forlidas Pond (Pensacola Mountains) and Lundström Lake (Shackleton Range), Antarctica Peeters, Karolien Hodgson, Dominic A Convey, Peter Willems, Anne 2011 application/pdf https://biblio.ugent.be/publication/1220529 http://hdl.handle.net/1854/LU-1220529 https://doi.org/10.1007/s00248-011-9842-7 https://biblio.ugent.be/publication/1220529/file/2097407 eng eng https://biblio.ugent.be/publication/1220529 http://hdl.handle.net/1854/LU-1220529 http://dx.doi.org/10.1007/s00248-011-9842-7 https://biblio.ugent.be/publication/1220529/file/2097407 No license (in copyright) info:eu-repo/semantics/restrictedAccess MICROBIAL ECOLOGY ISSN: 0095-3628 Biology and Life Sciences GEN. NOV EMENDED DESCRIPTION SP-NOV ET-AL. 2003 CYANOBACTERIAL MAT SAMPLE MCMURDO DRY VALLEYS cyanobacterial mat 16S rRNA gene sequencing cultivation ASPA Transantarctic Mountains SEA-ICE PSYCHROPHILIC BACTERIA SPECIES DEFINITION MELTWATER PONDS journalArticle info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2011 ftunivgent https://doi.org/10.1007/s00248-011-9842-7 2023-09-06T22:25:46Z Cultivation techniques were used to study the heterotrophic bacterial diversity in two microbial mat samples originating from the littoral zone of two continental Antarctic lakes (Forlidas Pond and Lundström Lake) in the Dufek Massif (within the Pensacola Mountains group of the Transantarctic Mountains) and Shackleton Range, respectively. Nearly 800 isolates were picked after incubation on several growth media at different temperatures. They were grouped using a whole-genome fingerprinting technique, repetitive element palindromic-PCR and partial 16S rRNA gene sequencing. Phylogenetic analysis of the complete 16S rRNA gene sequences of 82 representatives showed that the isolates belonged to four major phylogenetic groups: Actinobacteria, Bacteroidetes, Proteobacteria and Firmicutes. A relatively large difference between the samples was apparent. Forlidas Pond is a completely frozen water body underlain by a hypersaline brine, with summer thaw forming a slightly saline littoral moat. This was reflected in the bacterial diversity with a dominance of isolates belonging to Firmicutes, whereas isolates from the freshwater Lundström Lake revealed a dominance of Actinobacteria. A total of 42 different genera were recovered, including first records from Antarctica for Albidiferax, Bosea, Curvibacter, Luteimonas, Ornithinibacillus, Pseudoxanthomonas, Sphingopyxis and Spirosoma. Additionally, a considerable number of potential new species and new genera were recovered distributed over different phylogenetic groups. For several species where previously only the type strain was available in cultivation, we report additional strains. Comparison with public databases showed that 72% of the phylotypes were cosmopolitan whereas 23% were apparently currently restricted to Antarctica. Furthermore, the majority of the Bacteroidetes phylotypes were restricted to Antarctica and an important part of these represent previously unknown species. Article in Journal/Newspaper Antarc* Antarctic Antarctica McMurdo Dry Valleys Sea ice Ghent University Academic Bibliography Antarctic Dufek Massif ENVELOPE(-52.500,-52.500,-82.500,-82.500) Forlidas Pond ENVELOPE(-51.318,-51.318,-82.453,-82.453) McMurdo Dry Valleys Pensacola Mountains ENVELOPE(-58.000,-58.000,-83.500,-83.500) Shackleton Shackleton Range ENVELOPE(-26.000,-26.000,-80.833,-80.833) Transantarctic Mountains Microbial Ecology 62 2 399 413 |
institution |
Open Polar |
collection |
Ghent University Academic Bibliography |
op_collection_id |
ftunivgent |
language |
English |
topic |
Biology and Life Sciences GEN. NOV EMENDED DESCRIPTION SP-NOV ET-AL. 2003 CYANOBACTERIAL MAT SAMPLE MCMURDO DRY VALLEYS cyanobacterial mat 16S rRNA gene sequencing cultivation ASPA Transantarctic Mountains SEA-ICE PSYCHROPHILIC BACTERIA SPECIES DEFINITION MELTWATER PONDS |
spellingShingle |
Biology and Life Sciences GEN. NOV EMENDED DESCRIPTION SP-NOV ET-AL. 2003 CYANOBACTERIAL MAT SAMPLE MCMURDO DRY VALLEYS cyanobacterial mat 16S rRNA gene sequencing cultivation ASPA Transantarctic Mountains SEA-ICE PSYCHROPHILIC BACTERIA SPECIES DEFINITION MELTWATER PONDS Peeters, Karolien Hodgson, Dominic A Convey, Peter Willems, Anne Culturable diversity of heterotrophic bacteria in Forlidas Pond (Pensacola Mountains) and Lundström Lake (Shackleton Range), Antarctica |
topic_facet |
Biology and Life Sciences GEN. NOV EMENDED DESCRIPTION SP-NOV ET-AL. 2003 CYANOBACTERIAL MAT SAMPLE MCMURDO DRY VALLEYS cyanobacterial mat 16S rRNA gene sequencing cultivation ASPA Transantarctic Mountains SEA-ICE PSYCHROPHILIC BACTERIA SPECIES DEFINITION MELTWATER PONDS |
description |
Cultivation techniques were used to study the heterotrophic bacterial diversity in two microbial mat samples originating from the littoral zone of two continental Antarctic lakes (Forlidas Pond and Lundström Lake) in the Dufek Massif (within the Pensacola Mountains group of the Transantarctic Mountains) and Shackleton Range, respectively. Nearly 800 isolates were picked after incubation on several growth media at different temperatures. They were grouped using a whole-genome fingerprinting technique, repetitive element palindromic-PCR and partial 16S rRNA gene sequencing. Phylogenetic analysis of the complete 16S rRNA gene sequences of 82 representatives showed that the isolates belonged to four major phylogenetic groups: Actinobacteria, Bacteroidetes, Proteobacteria and Firmicutes. A relatively large difference between the samples was apparent. Forlidas Pond is a completely frozen water body underlain by a hypersaline brine, with summer thaw forming a slightly saline littoral moat. This was reflected in the bacterial diversity with a dominance of isolates belonging to Firmicutes, whereas isolates from the freshwater Lundström Lake revealed a dominance of Actinobacteria. A total of 42 different genera were recovered, including first records from Antarctica for Albidiferax, Bosea, Curvibacter, Luteimonas, Ornithinibacillus, Pseudoxanthomonas, Sphingopyxis and Spirosoma. Additionally, a considerable number of potential new species and new genera were recovered distributed over different phylogenetic groups. For several species where previously only the type strain was available in cultivation, we report additional strains. Comparison with public databases showed that 72% of the phylotypes were cosmopolitan whereas 23% were apparently currently restricted to Antarctica. Furthermore, the majority of the Bacteroidetes phylotypes were restricted to Antarctica and an important part of these represent previously unknown species. |
format |
Article in Journal/Newspaper |
author |
Peeters, Karolien Hodgson, Dominic A Convey, Peter Willems, Anne |
author_facet |
Peeters, Karolien Hodgson, Dominic A Convey, Peter Willems, Anne |
author_sort |
Peeters, Karolien |
title |
Culturable diversity of heterotrophic bacteria in Forlidas Pond (Pensacola Mountains) and Lundström Lake (Shackleton Range), Antarctica |
title_short |
Culturable diversity of heterotrophic bacteria in Forlidas Pond (Pensacola Mountains) and Lundström Lake (Shackleton Range), Antarctica |
title_full |
Culturable diversity of heterotrophic bacteria in Forlidas Pond (Pensacola Mountains) and Lundström Lake (Shackleton Range), Antarctica |
title_fullStr |
Culturable diversity of heterotrophic bacteria in Forlidas Pond (Pensacola Mountains) and Lundström Lake (Shackleton Range), Antarctica |
title_full_unstemmed |
Culturable diversity of heterotrophic bacteria in Forlidas Pond (Pensacola Mountains) and Lundström Lake (Shackleton Range), Antarctica |
title_sort |
culturable diversity of heterotrophic bacteria in forlidas pond (pensacola mountains) and lundström lake (shackleton range), antarctica |
publishDate |
2011 |
url |
https://biblio.ugent.be/publication/1220529 http://hdl.handle.net/1854/LU-1220529 https://doi.org/10.1007/s00248-011-9842-7 https://biblio.ugent.be/publication/1220529/file/2097407 |
long_lat |
ENVELOPE(-52.500,-52.500,-82.500,-82.500) ENVELOPE(-51.318,-51.318,-82.453,-82.453) ENVELOPE(-58.000,-58.000,-83.500,-83.500) ENVELOPE(-26.000,-26.000,-80.833,-80.833) |
geographic |
Antarctic Dufek Massif Forlidas Pond McMurdo Dry Valleys Pensacola Mountains Shackleton Shackleton Range Transantarctic Mountains |
geographic_facet |
Antarctic Dufek Massif Forlidas Pond McMurdo Dry Valleys Pensacola Mountains Shackleton Shackleton Range Transantarctic Mountains |
genre |
Antarc* Antarctic Antarctica McMurdo Dry Valleys Sea ice |
genre_facet |
Antarc* Antarctic Antarctica McMurdo Dry Valleys Sea ice |
op_source |
MICROBIAL ECOLOGY ISSN: 0095-3628 |
op_relation |
https://biblio.ugent.be/publication/1220529 http://hdl.handle.net/1854/LU-1220529 http://dx.doi.org/10.1007/s00248-011-9842-7 https://biblio.ugent.be/publication/1220529/file/2097407 |
op_rights |
No license (in copyright) info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1007/s00248-011-9842-7 |
container_title |
Microbial Ecology |
container_volume |
62 |
container_issue |
2 |
container_start_page |
399 |
op_container_end_page |
413 |
_version_ |
1778533084193357824 |