Effect of continuous dietary administration of the Saccharomyces cerevisiae yeast, Amnn9, on Pacific oyster (Crassostrea gigas) juveniles: Immunological and histopathological findings after Vibrio coralliilyticus challenge
Yeast cells and their cell wall components, mainly beta-glucans, are strong immunostimulants in bivalves, however, it remains unclear whether long-term administration of yeast contributes to prolonged activity of bivalve immune systems. Understanding these innate immune mechanisms is essential to es...
Published in: | Aquaculture |
---|---|
Main Authors: | , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
ELSEVIER
2023
|
Subjects: | |
Online Access: | https://biblio.ugent.be/publication/01HCCFHEN11KS1GJ492B6D0S13 http://hdl.handle.net/1854/LU-01HCCFHEN11KS1GJ492B6D0S13 https://doi.org/10.1016/j.aquaculture.2023.739644 https://biblio.ugent.be/publication/01HCCFHEN11KS1GJ492B6D0S13/file/01HCCFKS0AQE14X8WJPE21THRW |
id |
ftunivgent:oai:archive.ugent.be:01HCCFHEN11KS1GJ492B6D0S13 |
---|---|
record_format |
openpolar |
spelling |
ftunivgent:oai:archive.ugent.be:01HCCFHEN11KS1GJ492B6D0S13 2023-11-05T03:41:27+01:00 Effect of continuous dietary administration of the Saccharomyces cerevisiae yeast, Amnn9, on Pacific oyster (Crassostrea gigas) juveniles: Immunological and histopathological findings after Vibrio coralliilyticus challenge Loor, Alfredo Bossier, Peter Wang, Dongdong De Bels, Lobke Van Den Broeck, Wim Nevejan, Nancy Declercq, Annelies 2023 application/pdf https://biblio.ugent.be/publication/01HCCFHEN11KS1GJ492B6D0S13 http://hdl.handle.net/1854/LU-01HCCFHEN11KS1GJ492B6D0S13 https://doi.org/10.1016/j.aquaculture.2023.739644 https://biblio.ugent.be/publication/01HCCFHEN11KS1GJ492B6D0S13/file/01HCCFKS0AQE14X8WJPE21THRW eng eng ELSEVIER https://biblio.ugent.be/publication/01HCCFHEN11KS1GJ492B6D0S13 http://hdl.handle.net/1854/LU-01HCCFHEN11KS1GJ492B6D0S13 http://doi.org/10.1016/j.aquaculture.2023.739644 https://biblio.ugent.be/publication/01HCCFHEN11KS1GJ492B6D0S13/file/01HCCFKS0AQE14X8WJPE21THRW No license (in copyright) info:eu-repo/semantics/restrictedAccess AQUACULTURE ISSN: 0044-8486 ISSN: 1873-5622 BINDING-PROTEIN LGBP BETA-GLUCAN IMMUNE-RESPONSE GENE-EXPRESSION MEDITERRANEAN MUSSEL LIPOPOLYSACCHARIDE-BINDING MYTILUS-GALLOPROVINCIALIS MOLECULAR-CLONING INFECTION PROCESS CDNA CLONING Crassostrea gigas Mutant yeast Immunostimulant Host tolerance Vibrio infection Histopathology journalArticle info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2023 ftunivgent https://doi.org/10.1016/j.aquaculture.2023.739644 2023-10-11T22:07:06Z Yeast cells and their cell wall components, mainly beta-glucans, are strong immunostimulants in bivalves, however, it remains unclear whether long-term administration of yeast contributes to prolonged activity of bivalve immune systems. Understanding these innate immune mechanisms is essential to establishing feeding strategies and disease prevention in hatcheries and nurseries. The present study examined the immunostimulatory effect of continuous dietary supply of the Saccharomyces cerevisiae mnn9 mutant (Amnn9), which exhibits elevated exposure of beta-glucans on the cell-wall surface, on Crassostrea gigas juveniles, and its protective effect against Vibrio coralliilyticus infections. In trial 1 the expression levels of immune-related genes were determined in oysters fed the Amnn9-containing diet 50A50Y (50% algae, 50% Amnn9; based on dry weight), compared to those fed the 100% algae-based diet (100A), after 6, 9, 12, 18, 24 h of feeding. In trial 2: the feeding period was expanded to 8 days, where the algal diet (100A) was provided every day, but substituted with 50A50Y for 0, 1, 2, 4, and 8 days (treatments 0D (control), 1D, 2D, 4D, and 8D, respectively) just before a Vibrio challenge, which was monitored for 7 days. Gene expression was measured before and 24-h after the Vibrio challenge. Histological analysis was performed on oysters on day 7 post-Vibrio challenge. Results showed that 50A50Y induced upregulation of interleukin 17-5 (CgIL17-5; 6 h), lysozyme (CgLys; 6 and 9 h), beta-glucan-binding protein (Cg beta GBP; 18 h), and superoxide dismutase (CgSOD; 18 and 24 h) genes in juveniles (trial 1). Before the challenge, trial 2 demonstrated overexpression of CgSOD in animals from 1D, CgLys in those from 1D, 2D, and 4D, but not in oysters from 8D, compared to those from 0D. However, higher CgIL17-5 expression levels were observed in animals from 2D, 4D, and 8D, suggesting that inflammatory genes can remain inducible after 8 days of Amnn9 feeding. After the Vibrio challenge, a significantly higher survival ... Article in Journal/Newspaper Crassostrea gigas Pacific oyster Ghent University Academic Bibliography Aquaculture 574 739644 |
institution |
Open Polar |
collection |
Ghent University Academic Bibliography |
op_collection_id |
ftunivgent |
language |
English |
topic |
BINDING-PROTEIN LGBP BETA-GLUCAN IMMUNE-RESPONSE GENE-EXPRESSION MEDITERRANEAN MUSSEL LIPOPOLYSACCHARIDE-BINDING MYTILUS-GALLOPROVINCIALIS MOLECULAR-CLONING INFECTION PROCESS CDNA CLONING Crassostrea gigas Mutant yeast Immunostimulant Host tolerance Vibrio infection Histopathology |
spellingShingle |
BINDING-PROTEIN LGBP BETA-GLUCAN IMMUNE-RESPONSE GENE-EXPRESSION MEDITERRANEAN MUSSEL LIPOPOLYSACCHARIDE-BINDING MYTILUS-GALLOPROVINCIALIS MOLECULAR-CLONING INFECTION PROCESS CDNA CLONING Crassostrea gigas Mutant yeast Immunostimulant Host tolerance Vibrio infection Histopathology Loor, Alfredo Bossier, Peter Wang, Dongdong De Bels, Lobke Van Den Broeck, Wim Nevejan, Nancy Declercq, Annelies Effect of continuous dietary administration of the Saccharomyces cerevisiae yeast, Amnn9, on Pacific oyster (Crassostrea gigas) juveniles: Immunological and histopathological findings after Vibrio coralliilyticus challenge |
topic_facet |
BINDING-PROTEIN LGBP BETA-GLUCAN IMMUNE-RESPONSE GENE-EXPRESSION MEDITERRANEAN MUSSEL LIPOPOLYSACCHARIDE-BINDING MYTILUS-GALLOPROVINCIALIS MOLECULAR-CLONING INFECTION PROCESS CDNA CLONING Crassostrea gigas Mutant yeast Immunostimulant Host tolerance Vibrio infection Histopathology |
description |
Yeast cells and their cell wall components, mainly beta-glucans, are strong immunostimulants in bivalves, however, it remains unclear whether long-term administration of yeast contributes to prolonged activity of bivalve immune systems. Understanding these innate immune mechanisms is essential to establishing feeding strategies and disease prevention in hatcheries and nurseries. The present study examined the immunostimulatory effect of continuous dietary supply of the Saccharomyces cerevisiae mnn9 mutant (Amnn9), which exhibits elevated exposure of beta-glucans on the cell-wall surface, on Crassostrea gigas juveniles, and its protective effect against Vibrio coralliilyticus infections. In trial 1 the expression levels of immune-related genes were determined in oysters fed the Amnn9-containing diet 50A50Y (50% algae, 50% Amnn9; based on dry weight), compared to those fed the 100% algae-based diet (100A), after 6, 9, 12, 18, 24 h of feeding. In trial 2: the feeding period was expanded to 8 days, where the algal diet (100A) was provided every day, but substituted with 50A50Y for 0, 1, 2, 4, and 8 days (treatments 0D (control), 1D, 2D, 4D, and 8D, respectively) just before a Vibrio challenge, which was monitored for 7 days. Gene expression was measured before and 24-h after the Vibrio challenge. Histological analysis was performed on oysters on day 7 post-Vibrio challenge. Results showed that 50A50Y induced upregulation of interleukin 17-5 (CgIL17-5; 6 h), lysozyme (CgLys; 6 and 9 h), beta-glucan-binding protein (Cg beta GBP; 18 h), and superoxide dismutase (CgSOD; 18 and 24 h) genes in juveniles (trial 1). Before the challenge, trial 2 demonstrated overexpression of CgSOD in animals from 1D, CgLys in those from 1D, 2D, and 4D, but not in oysters from 8D, compared to those from 0D. However, higher CgIL17-5 expression levels were observed in animals from 2D, 4D, and 8D, suggesting that inflammatory genes can remain inducible after 8 days of Amnn9 feeding. After the Vibrio challenge, a significantly higher survival ... |
format |
Article in Journal/Newspaper |
author |
Loor, Alfredo Bossier, Peter Wang, Dongdong De Bels, Lobke Van Den Broeck, Wim Nevejan, Nancy Declercq, Annelies |
author_facet |
Loor, Alfredo Bossier, Peter Wang, Dongdong De Bels, Lobke Van Den Broeck, Wim Nevejan, Nancy Declercq, Annelies |
author_sort |
Loor, Alfredo |
title |
Effect of continuous dietary administration of the Saccharomyces cerevisiae yeast, Amnn9, on Pacific oyster (Crassostrea gigas) juveniles: Immunological and histopathological findings after Vibrio coralliilyticus challenge |
title_short |
Effect of continuous dietary administration of the Saccharomyces cerevisiae yeast, Amnn9, on Pacific oyster (Crassostrea gigas) juveniles: Immunological and histopathological findings after Vibrio coralliilyticus challenge |
title_full |
Effect of continuous dietary administration of the Saccharomyces cerevisiae yeast, Amnn9, on Pacific oyster (Crassostrea gigas) juveniles: Immunological and histopathological findings after Vibrio coralliilyticus challenge |
title_fullStr |
Effect of continuous dietary administration of the Saccharomyces cerevisiae yeast, Amnn9, on Pacific oyster (Crassostrea gigas) juveniles: Immunological and histopathological findings after Vibrio coralliilyticus challenge |
title_full_unstemmed |
Effect of continuous dietary administration of the Saccharomyces cerevisiae yeast, Amnn9, on Pacific oyster (Crassostrea gigas) juveniles: Immunological and histopathological findings after Vibrio coralliilyticus challenge |
title_sort |
effect of continuous dietary administration of the saccharomyces cerevisiae yeast, amnn9, on pacific oyster (crassostrea gigas) juveniles: immunological and histopathological findings after vibrio coralliilyticus challenge |
publisher |
ELSEVIER |
publishDate |
2023 |
url |
https://biblio.ugent.be/publication/01HCCFHEN11KS1GJ492B6D0S13 http://hdl.handle.net/1854/LU-01HCCFHEN11KS1GJ492B6D0S13 https://doi.org/10.1016/j.aquaculture.2023.739644 https://biblio.ugent.be/publication/01HCCFHEN11KS1GJ492B6D0S13/file/01HCCFKS0AQE14X8WJPE21THRW |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
AQUACULTURE ISSN: 0044-8486 ISSN: 1873-5622 |
op_relation |
https://biblio.ugent.be/publication/01HCCFHEN11KS1GJ492B6D0S13 http://hdl.handle.net/1854/LU-01HCCFHEN11KS1GJ492B6D0S13 http://doi.org/10.1016/j.aquaculture.2023.739644 https://biblio.ugent.be/publication/01HCCFHEN11KS1GJ492B6D0S13/file/01HCCFKS0AQE14X8WJPE21THRW |
op_rights |
No license (in copyright) info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1016/j.aquaculture.2023.739644 |
container_title |
Aquaculture |
container_volume |
574 |
container_start_page |
739644 |
_version_ |
1781697809058103296 |