Unraveling the microbiota of the fish parasite Pseudoterranova decipiens in codfish (Gadus morhua) reveals a fish-related bacterial community
Anisakidae, mainly represented by the species Anisakis simplex and Pseudoterranova decipiens, are one of the most commonly zoonotic nematodes present in marine fish species. Apart from public health risks directly linked to the parasite itself, little is known on the effects of the migrating nematod...
Published in: | International Journal of Food Microbiology |
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Format: | Article in Journal/Newspaper |
Language: | English |
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2022
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Online Access: | https://biblio.ugent.be/publication/01GM2VS8T9QDFZ89GDH3KPMAA3 http://hdl.handle.net/1854/LU-01GM2VS8T9QDFZ89GDH3KPMAA3 https://doi.org/10.1016/j.ijfoodmicro.2022.109591 https://biblio.ugent.be/publication/01GM2VS8T9QDFZ89GDH3KPMAA3/file/01GR1CFSEG8TDAEEZ0HRFN94WF |
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ftunivgent:oai:archive.ugent.be:01GM2VS8T9QDFZ89GDH3KPMAA3 2023-10-01T03:56:05+02:00 Unraveling the microbiota of the fish parasite Pseudoterranova decipiens in codfish (Gadus morhua) reveals a fish-related bacterial community Saelens, Ganna Houf, Kurt 2022 application/pdf https://biblio.ugent.be/publication/01GM2VS8T9QDFZ89GDH3KPMAA3 http://hdl.handle.net/1854/LU-01GM2VS8T9QDFZ89GDH3KPMAA3 https://doi.org/10.1016/j.ijfoodmicro.2022.109591 https://biblio.ugent.be/publication/01GM2VS8T9QDFZ89GDH3KPMAA3/file/01GR1CFSEG8TDAEEZ0HRFN94WF eng eng https://biblio.ugent.be/publication/01GM2VS8T9QDFZ89GDH3KPMAA3 http://hdl.handle.net/1854/LU-01GM2VS8T9QDFZ89GDH3KPMAA3 http://dx.doi.org/10.1016/j.ijfoodmicro.2022.109591 https://biblio.ugent.be/publication/01GM2VS8T9QDFZ89GDH3KPMAA3/file/01GR1CFSEG8TDAEEZ0HRFN94WF No license (in copyright) info:eu-repo/semantics/restrictedAccess INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY ISSN: 0168-1605 ISSN: 1879-3460 Veterinary Sciences SPOILAGE MICROBIOTA MORGANELLA-MORGANII ANISAKIS-SIMPLEX SHELF-LIFE SP NOV IDENTIFICATION LARVAE CLASSIFICATION PSYCHROBACTER AEROMONAS Anisakidae Pseudoterranova decipiens Codfish Microbiota Spoilage journalArticle info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2022 ftunivgent https://doi.org/10.1016/j.ijfoodmicro.2022.109591 2023-09-06T22:53:47Z Anisakidae, mainly represented by the species Anisakis simplex and Pseudoterranova decipiens, are one of the most commonly zoonotic nematodes present in marine fish species. Apart from public health risks directly linked to the parasite itself, little is known on the effects of the migrating nematodes on the hygienic quality of the fish fillet due to bacteria it carries. In the present study, the cultivated bacterial community on and in individual P. decipiens larvae deriving from codfish is reported. Four isolation media were included and evaluated to increase the bacterial diversity isolated, and identification of the bacterial growth was performed by a combination of Matrix-Assisted Laser Desorption Ionization Time-Of-Flight mass spectrometry and 16S rRNA gene sequencing. Results revealed that the microbiota of P. decipiens larvae comprises both potential spoilage bacteria and human opportunistic pathogens, and that a combined isolation on the general isolation medium tryptone soy agar and a medium supplemented with artificial seawater resulted in the highest bacterial recovery in terms of diversity and enumeration. Dissimilarity analysis also revealed similar, though unique, bacterial communities between nematodes originating from the same fish suggesting that anisakid microbiota compositions are reflections of the microbial assemblages in the fish host as an individual, and that the gut microbiome is diverse within gadoid fish species originating from the same geographical habitat. Future research should, based on the results in the present study, further elaborate on the comparison of the bacterial communities of both the larva and the codfish from which it was isolated, and, explore the extrapolation potential towards other fish and nematode species. Also, the actual degree of risk beyond the simple presence of the parasite due to carriage of opportunistic bacteria should be examined, as well as the nematode's true effect on spoilage. Article in Journal/Newspaper Gadus morhua Ghent University Academic Bibliography International Journal of Food Microbiology 367 109591 |
institution |
Open Polar |
collection |
Ghent University Academic Bibliography |
op_collection_id |
ftunivgent |
language |
English |
topic |
Veterinary Sciences SPOILAGE MICROBIOTA MORGANELLA-MORGANII ANISAKIS-SIMPLEX SHELF-LIFE SP NOV IDENTIFICATION LARVAE CLASSIFICATION PSYCHROBACTER AEROMONAS Anisakidae Pseudoterranova decipiens Codfish Microbiota Spoilage |
spellingShingle |
Veterinary Sciences SPOILAGE MICROBIOTA MORGANELLA-MORGANII ANISAKIS-SIMPLEX SHELF-LIFE SP NOV IDENTIFICATION LARVAE CLASSIFICATION PSYCHROBACTER AEROMONAS Anisakidae Pseudoterranova decipiens Codfish Microbiota Spoilage Saelens, Ganna Houf, Kurt Unraveling the microbiota of the fish parasite Pseudoterranova decipiens in codfish (Gadus morhua) reveals a fish-related bacterial community |
topic_facet |
Veterinary Sciences SPOILAGE MICROBIOTA MORGANELLA-MORGANII ANISAKIS-SIMPLEX SHELF-LIFE SP NOV IDENTIFICATION LARVAE CLASSIFICATION PSYCHROBACTER AEROMONAS Anisakidae Pseudoterranova decipiens Codfish Microbiota Spoilage |
description |
Anisakidae, mainly represented by the species Anisakis simplex and Pseudoterranova decipiens, are one of the most commonly zoonotic nematodes present in marine fish species. Apart from public health risks directly linked to the parasite itself, little is known on the effects of the migrating nematodes on the hygienic quality of the fish fillet due to bacteria it carries. In the present study, the cultivated bacterial community on and in individual P. decipiens larvae deriving from codfish is reported. Four isolation media were included and evaluated to increase the bacterial diversity isolated, and identification of the bacterial growth was performed by a combination of Matrix-Assisted Laser Desorption Ionization Time-Of-Flight mass spectrometry and 16S rRNA gene sequencing. Results revealed that the microbiota of P. decipiens larvae comprises both potential spoilage bacteria and human opportunistic pathogens, and that a combined isolation on the general isolation medium tryptone soy agar and a medium supplemented with artificial seawater resulted in the highest bacterial recovery in terms of diversity and enumeration. Dissimilarity analysis also revealed similar, though unique, bacterial communities between nematodes originating from the same fish suggesting that anisakid microbiota compositions are reflections of the microbial assemblages in the fish host as an individual, and that the gut microbiome is diverse within gadoid fish species originating from the same geographical habitat. Future research should, based on the results in the present study, further elaborate on the comparison of the bacterial communities of both the larva and the codfish from which it was isolated, and, explore the extrapolation potential towards other fish and nematode species. Also, the actual degree of risk beyond the simple presence of the parasite due to carriage of opportunistic bacteria should be examined, as well as the nematode's true effect on spoilage. |
format |
Article in Journal/Newspaper |
author |
Saelens, Ganna Houf, Kurt |
author_facet |
Saelens, Ganna Houf, Kurt |
author_sort |
Saelens, Ganna |
title |
Unraveling the microbiota of the fish parasite Pseudoterranova decipiens in codfish (Gadus morhua) reveals a fish-related bacterial community |
title_short |
Unraveling the microbiota of the fish parasite Pseudoterranova decipiens in codfish (Gadus morhua) reveals a fish-related bacterial community |
title_full |
Unraveling the microbiota of the fish parasite Pseudoterranova decipiens in codfish (Gadus morhua) reveals a fish-related bacterial community |
title_fullStr |
Unraveling the microbiota of the fish parasite Pseudoterranova decipiens in codfish (Gadus morhua) reveals a fish-related bacterial community |
title_full_unstemmed |
Unraveling the microbiota of the fish parasite Pseudoterranova decipiens in codfish (Gadus morhua) reveals a fish-related bacterial community |
title_sort |
unraveling the microbiota of the fish parasite pseudoterranova decipiens in codfish (gadus morhua) reveals a fish-related bacterial community |
publishDate |
2022 |
url |
https://biblio.ugent.be/publication/01GM2VS8T9QDFZ89GDH3KPMAA3 http://hdl.handle.net/1854/LU-01GM2VS8T9QDFZ89GDH3KPMAA3 https://doi.org/10.1016/j.ijfoodmicro.2022.109591 https://biblio.ugent.be/publication/01GM2VS8T9QDFZ89GDH3KPMAA3/file/01GR1CFSEG8TDAEEZ0HRFN94WF |
genre |
Gadus morhua |
genre_facet |
Gadus morhua |
op_source |
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY ISSN: 0168-1605 ISSN: 1879-3460 |
op_relation |
https://biblio.ugent.be/publication/01GM2VS8T9QDFZ89GDH3KPMAA3 http://hdl.handle.net/1854/LU-01GM2VS8T9QDFZ89GDH3KPMAA3 http://dx.doi.org/10.1016/j.ijfoodmicro.2022.109591 https://biblio.ugent.be/publication/01GM2VS8T9QDFZ89GDH3KPMAA3/file/01GR1CFSEG8TDAEEZ0HRFN94WF |
op_rights |
No license (in copyright) info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1016/j.ijfoodmicro.2022.109591 |
container_title |
International Journal of Food Microbiology |
container_volume |
367 |
container_start_page |
109591 |
_version_ |
1778525201736138752 |