Comparative 16SrDNA Gene-Based Microbiota Profiles of the Pacific Oyster (Crassostrea gigas) and the Mediterranean Mussel (Mytilus galloprovincialis) from a Shellfish Farm (Ligurian Sea, Italy)
The pacific oyster Crassostrea gigas and the Mediterranean mussel Mytilus galloprovincialis are two widely farmed bivalve species which show contrasting behaviour in relation to microbial diseases, with C. gigas being more susceptible and M. galloprovincialis being generally resistant. In a recent s...
Published in: | Microbial Ecology |
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Format: | Article in Journal/Newspaper |
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2018
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Online Access: | http://hdl.handle.net/11567/876266 https://doi.org/10.1007/s00248-017-1051-6 |
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ftunivgenova:oai:iris.unige.it:11567/876266 2024-04-14T08:10:41+00:00 Comparative 16SrDNA Gene-Based Microbiota Profiles of the Pacific Oyster (Crassostrea gigas) and the Mediterranean Mussel (Mytilus galloprovincialis) from a Shellfish Farm (Ligurian Sea, Italy) VEZZULLI, LUIGI STAGNARO, LAURA GRANDE, CHIARA TASSISTRO, GIOVANNI CANESI, LAURA PRUZZO, CARLA Vezzulli, Luigi Stagnaro, Laura Grande, Chiara Tassistro, Giovanni Canesi, Laura Pruzzo, Carla 2018 ELETTRONICO http://hdl.handle.net/11567/876266 https://doi.org/10.1007/s00248-017-1051-6 eng eng Springer New York LLC info:eu-repo/semantics/altIdentifier/pmid/28803409 info:eu-repo/semantics/altIdentifier/wos/WOS:000418740200020 volume:75 (2) firstpage:495 lastpage:504 numberofpages:10 journal:MICROBIAL ECOLOGY info:eu-repo/grantAgreement/EC/H2020/678589 http://hdl.handle.net/11567/876266 doi:10.1007/s00248-017-1051-6 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85027378839 info:eu-repo/semantics/openAccess 16SrDNA Crassostrea giga Microbiota Mytilus galloprovinciali Next generation sequencing Ecology Evolution Behavior and Systematic Soil Science info:eu-repo/semantics/article 2018 ftunivgenova https://doi.org/10.1007/s00248-017-1051-6 2024-03-21T02:29:06Z The pacific oyster Crassostrea gigas and the Mediterranean mussel Mytilus galloprovincialis are two widely farmed bivalve species which show contrasting behaviour in relation to microbial diseases, with C. gigas being more susceptible and M. galloprovincialis being generally resistant. In a recent study, we showed that different susceptibility to infection exhibited by these two bivalve species may depend on their different capability to kill invading pathogens (e.g., Vibrio spp.) through the action of haemolymph components. Specific microbial-host interactions may also impact bivalve microbiome structure and further influence susceptibility/resistance to microbial diseases. To further investigate this concept, a comparative study of haemolymph and digestive gland 16SrDNA gene-based bacterial microbiota profiles in C. gigas and M. galloprovincialis co-cultivated at the same aquaculture site was carried out using pyrosequencing. Bacterial communities associated with bivalve tissues (hemolymph and digestive gland) were significantly different from those of seawater, and were dominated by relatively few genera such as Vibrio and Pseudoalteromonas. In general, Vibrio accounted for a larger fraction of the microbiota in C. gigas (on average 1.7-fold in the haemolymph) compared to M. galloprovincialis, suggesting that C. gigas may provide better conditions for survival for these bacteria, including potential pathogenic species such as V. aestuarianus. Vibrios appeared to be important members of C. gigas and M. galloprovincialis microbiota and might play a contrasting role in health and disease of bivalve species. Accordingly, microbiome analyses performed on bivalve specimens subjected to commercial depuration highlighted the ineffectiveness of such practice in removing Vibrio species from bivalve tissues. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Università degli Studi di Genova: CINECA IRIS Pacific Microbial Ecology 75 2 495 504 |
institution |
Open Polar |
collection |
Università degli Studi di Genova: CINECA IRIS |
op_collection_id |
ftunivgenova |
language |
English |
topic |
16SrDNA Crassostrea giga Microbiota Mytilus galloprovinciali Next generation sequencing Ecology Evolution Behavior and Systematic Soil Science |
spellingShingle |
16SrDNA Crassostrea giga Microbiota Mytilus galloprovinciali Next generation sequencing Ecology Evolution Behavior and Systematic Soil Science VEZZULLI, LUIGI STAGNARO, LAURA GRANDE, CHIARA TASSISTRO, GIOVANNI CANESI, LAURA PRUZZO, CARLA Comparative 16SrDNA Gene-Based Microbiota Profiles of the Pacific Oyster (Crassostrea gigas) and the Mediterranean Mussel (Mytilus galloprovincialis) from a Shellfish Farm (Ligurian Sea, Italy) |
topic_facet |
16SrDNA Crassostrea giga Microbiota Mytilus galloprovinciali Next generation sequencing Ecology Evolution Behavior and Systematic Soil Science |
description |
The pacific oyster Crassostrea gigas and the Mediterranean mussel Mytilus galloprovincialis are two widely farmed bivalve species which show contrasting behaviour in relation to microbial diseases, with C. gigas being more susceptible and M. galloprovincialis being generally resistant. In a recent study, we showed that different susceptibility to infection exhibited by these two bivalve species may depend on their different capability to kill invading pathogens (e.g., Vibrio spp.) through the action of haemolymph components. Specific microbial-host interactions may also impact bivalve microbiome structure and further influence susceptibility/resistance to microbial diseases. To further investigate this concept, a comparative study of haemolymph and digestive gland 16SrDNA gene-based bacterial microbiota profiles in C. gigas and M. galloprovincialis co-cultivated at the same aquaculture site was carried out using pyrosequencing. Bacterial communities associated with bivalve tissues (hemolymph and digestive gland) were significantly different from those of seawater, and were dominated by relatively few genera such as Vibrio and Pseudoalteromonas. In general, Vibrio accounted for a larger fraction of the microbiota in C. gigas (on average 1.7-fold in the haemolymph) compared to M. galloprovincialis, suggesting that C. gigas may provide better conditions for survival for these bacteria, including potential pathogenic species such as V. aestuarianus. Vibrios appeared to be important members of C. gigas and M. galloprovincialis microbiota and might play a contrasting role in health and disease of bivalve species. Accordingly, microbiome analyses performed on bivalve specimens subjected to commercial depuration highlighted the ineffectiveness of such practice in removing Vibrio species from bivalve tissues. |
author2 |
Vezzulli, Luigi Stagnaro, Laura Grande, Chiara Tassistro, Giovanni Canesi, Laura Pruzzo, Carla |
format |
Article in Journal/Newspaper |
author |
VEZZULLI, LUIGI STAGNARO, LAURA GRANDE, CHIARA TASSISTRO, GIOVANNI CANESI, LAURA PRUZZO, CARLA |
author_facet |
VEZZULLI, LUIGI STAGNARO, LAURA GRANDE, CHIARA TASSISTRO, GIOVANNI CANESI, LAURA PRUZZO, CARLA |
author_sort |
VEZZULLI, LUIGI |
title |
Comparative 16SrDNA Gene-Based Microbiota Profiles of the Pacific Oyster (Crassostrea gigas) and the Mediterranean Mussel (Mytilus galloprovincialis) from a Shellfish Farm (Ligurian Sea, Italy) |
title_short |
Comparative 16SrDNA Gene-Based Microbiota Profiles of the Pacific Oyster (Crassostrea gigas) and the Mediterranean Mussel (Mytilus galloprovincialis) from a Shellfish Farm (Ligurian Sea, Italy) |
title_full |
Comparative 16SrDNA Gene-Based Microbiota Profiles of the Pacific Oyster (Crassostrea gigas) and the Mediterranean Mussel (Mytilus galloprovincialis) from a Shellfish Farm (Ligurian Sea, Italy) |
title_fullStr |
Comparative 16SrDNA Gene-Based Microbiota Profiles of the Pacific Oyster (Crassostrea gigas) and the Mediterranean Mussel (Mytilus galloprovincialis) from a Shellfish Farm (Ligurian Sea, Italy) |
title_full_unstemmed |
Comparative 16SrDNA Gene-Based Microbiota Profiles of the Pacific Oyster (Crassostrea gigas) and the Mediterranean Mussel (Mytilus galloprovincialis) from a Shellfish Farm (Ligurian Sea, Italy) |
title_sort |
comparative 16srdna gene-based microbiota profiles of the pacific oyster (crassostrea gigas) and the mediterranean mussel (mytilus galloprovincialis) from a shellfish farm (ligurian sea, italy) |
publisher |
Springer New York LLC |
publishDate |
2018 |
url |
http://hdl.handle.net/11567/876266 https://doi.org/10.1007/s00248-017-1051-6 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_relation |
info:eu-repo/semantics/altIdentifier/pmid/28803409 info:eu-repo/semantics/altIdentifier/wos/WOS:000418740200020 volume:75 (2) firstpage:495 lastpage:504 numberofpages:10 journal:MICROBIAL ECOLOGY info:eu-repo/grantAgreement/EC/H2020/678589 http://hdl.handle.net/11567/876266 doi:10.1007/s00248-017-1051-6 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85027378839 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1007/s00248-017-1051-6 |
container_title |
Microbial Ecology |
container_volume |
75 |
container_issue |
2 |
container_start_page |
495 |
op_container_end_page |
504 |
_version_ |
1796308315179843584 |