Production, purification, and characterization of an extracellular acid protease from the marine Antarctic yeast Rhodotorula mucilaginosa L7
The production, purification, and characterization of an extracellular protease released by Rhodotorula mucilaginosa L7 were evaluated in this study. This strain was isolated from an Antarctic marine alga and previously selected among others based on the capacity to produce the highest extracellular...
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ftunivgenova:oai:iris.unige.it:11567/819702 2024-04-21T07:51:25+00:00 Production, purification, and characterization of an extracellular acid protease from the marine Antarctic yeast Rhodotorula mucilaginosa L7 Lario, L. D. Chaud, L. Almeida, M. G. CONVERTI, ATTILIO Sette, L. D. Pessoa Jr, A. . Lario, L. D. Chaud, L. Almeida, M. G. Converti, Attilio Sette, L. D. Pessoa Jr, A. . 2015 ELETTRONICO http://hdl.handle.net/11567/819702 https://doi.org/10.1016/j.funbio.2015.08.012 eng eng Elsevier country:GBR place:Oxford info:eu-repo/semantics/altIdentifier/pmid/26466885 info:eu-repo/semantics/altIdentifier/wos/WOS:000364263800014 volume:119(11) firstpage:1129 lastpage:1136 numberofpages:8 journal:FUNGAL BIOLOGY http://hdl.handle.net/11567/819702 doi:10.1016/j.funbio.2015.08.012 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-84945460099 info:eu-repo/semantics/closedAccess cold-adapted yeast enzyme characterization extracellular protease info:eu-repo/semantics/article 2015 ftunivgenova https://doi.org/10.1016/j.funbio.2015.08.012 2024-03-28T01:17:14Z The production, purification, and characterization of an extracellular protease released by Rhodotorula mucilaginosa L7 were evaluated in this study. This strain was isolated from an Antarctic marine alga and previously selected among others based on the capacity to produce the highest extracellular proteolytic activity in preliminary tests. R. mucilaginosa L7 was grown in Saboraud-dextrose medium at 25 °C, and the cell growth, pH of the medium, extracellular protease production and the glucose and protein consumption were determined as a function of time. The protease was then purified, and the effects of pH, temperature, and salt concentration on the catalytic activity and enzyme stability were determined. Enzyme production started at the beginning of the exponential phase of growth and reached a maximum after 48 h, which was accompanied by a decrease in the pH as well as reductions of the protein and glucose concentrations in the medium. The purified protease presented optimal catalytic activity at pH 5.0 and 50 °C. Finally, the enzyme was stable in the presence of high concentrations of NaCl. These characteristics are of interest for future studies and may lead to potential biotechnological applications that require enzyme activity and stability under acidic conditions and/or high salt concentrations. Article in Journal/Newspaper Antarc* Antarctic Università degli Studi di Genova: CINECA IRIS Fungal Biology 119 11 1129 1136 |
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Open Polar |
collection |
Università degli Studi di Genova: CINECA IRIS |
op_collection_id |
ftunivgenova |
language |
English |
topic |
cold-adapted yeast enzyme characterization extracellular protease |
spellingShingle |
cold-adapted yeast enzyme characterization extracellular protease Lario, L. D. Chaud, L. Almeida, M. G. CONVERTI, ATTILIO Sette, L. D. Pessoa Jr, A. . Production, purification, and characterization of an extracellular acid protease from the marine Antarctic yeast Rhodotorula mucilaginosa L7 |
topic_facet |
cold-adapted yeast enzyme characterization extracellular protease |
description |
The production, purification, and characterization of an extracellular protease released by Rhodotorula mucilaginosa L7 were evaluated in this study. This strain was isolated from an Antarctic marine alga and previously selected among others based on the capacity to produce the highest extracellular proteolytic activity in preliminary tests. R. mucilaginosa L7 was grown in Saboraud-dextrose medium at 25 °C, and the cell growth, pH of the medium, extracellular protease production and the glucose and protein consumption were determined as a function of time. The protease was then purified, and the effects of pH, temperature, and salt concentration on the catalytic activity and enzyme stability were determined. Enzyme production started at the beginning of the exponential phase of growth and reached a maximum after 48 h, which was accompanied by a decrease in the pH as well as reductions of the protein and glucose concentrations in the medium. The purified protease presented optimal catalytic activity at pH 5.0 and 50 °C. Finally, the enzyme was stable in the presence of high concentrations of NaCl. These characteristics are of interest for future studies and may lead to potential biotechnological applications that require enzyme activity and stability under acidic conditions and/or high salt concentrations. |
author2 |
Lario, L. D. Chaud, L. Almeida, M. G. Converti, Attilio Sette, L. D. Pessoa Jr, A. . |
format |
Article in Journal/Newspaper |
author |
Lario, L. D. Chaud, L. Almeida, M. G. CONVERTI, ATTILIO Sette, L. D. Pessoa Jr, A. . |
author_facet |
Lario, L. D. Chaud, L. Almeida, M. G. CONVERTI, ATTILIO Sette, L. D. Pessoa Jr, A. . |
author_sort |
Lario, L. D. |
title |
Production, purification, and characterization of an extracellular acid protease from the marine Antarctic yeast Rhodotorula mucilaginosa L7 |
title_short |
Production, purification, and characterization of an extracellular acid protease from the marine Antarctic yeast Rhodotorula mucilaginosa L7 |
title_full |
Production, purification, and characterization of an extracellular acid protease from the marine Antarctic yeast Rhodotorula mucilaginosa L7 |
title_fullStr |
Production, purification, and characterization of an extracellular acid protease from the marine Antarctic yeast Rhodotorula mucilaginosa L7 |
title_full_unstemmed |
Production, purification, and characterization of an extracellular acid protease from the marine Antarctic yeast Rhodotorula mucilaginosa L7 |
title_sort |
production, purification, and characterization of an extracellular acid protease from the marine antarctic yeast rhodotorula mucilaginosa l7 |
publisher |
Elsevier |
publishDate |
2015 |
url |
http://hdl.handle.net/11567/819702 https://doi.org/10.1016/j.funbio.2015.08.012 |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
info:eu-repo/semantics/altIdentifier/pmid/26466885 info:eu-repo/semantics/altIdentifier/wos/WOS:000364263800014 volume:119(11) firstpage:1129 lastpage:1136 numberofpages:8 journal:FUNGAL BIOLOGY http://hdl.handle.net/11567/819702 doi:10.1016/j.funbio.2015.08.012 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-84945460099 |
op_rights |
info:eu-repo/semantics/closedAccess |
op_doi |
https://doi.org/10.1016/j.funbio.2015.08.012 |
container_title |
Fungal Biology |
container_volume |
119 |
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11 |
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1129 |
op_container_end_page |
1136 |
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1796934777307136000 |