Preliminary investigation on cholinesterase activity in Adamussium colbecki from Terra Nova Bay: field and laboratory study
While the inhibition of cholinesterase (ChE) activity as a biomarker of exposure to neurotoxic insecticides is well established in aquatic invertebrates of temperate areas, little is known about organisms from polar regions including Antarctica. Cholinesterase activity was investigated in specimens...
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Editore Gordon and Breach Science Publishers Taylor & Francis Limited:Rankine Road, Basingstoke RG24 8PR United Kingdom:011 44 1256 813035, EMAIL: madeline.sims@tandf.co.uk, info@tandf.co.uk, INTERNET: http://www.tandf.co.uk, Fax: 011 44 1256 330245
2004
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ftunivgenova:oai:iris.unige.it:11567/213053 2024-02-11T09:56:56+01:00 Preliminary investigation on cholinesterase activity in Adamussium colbecki from Terra Nova Bay: field and laboratory study CORSI I. S. BONACCI G. SANTOVITO L. CASTAGNOLO S. FOCARDI CHIANTORE, MARIACHIARA Corsi, I. S., Bonacci G., Santovito Chiantore, Mariachiara L., Castagnolo S., Focardi 2004 STAMPA http://hdl.handle.net/11567/213053 eng eng Editore Gordon and Breach Science Publishers Taylor & Francis Limited:Rankine Road, Basingstoke RG24 8PR United Kingdom:011 44 1256 813035, EMAIL: madeline.sims@tandf.co.uk, info@tandf.co.uk, INTERNET: http://www.tandf.co.uk, Fax: 011 44 1256 330245 volume:20 (Suppl 1) firstpage:79 lastpage:87 journal:CHEMISTRY IN ECOLOGY http://hdl.handle.net/11567/213053 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-5044252695 Adamussium colbecki Cholinesterase Antarctica Biomarker info:eu-repo/semantics/article 2004 ftunivgenova 2024-01-17T17:34:41Z While the inhibition of cholinesterase (ChE) activity as a biomarker of exposure to neurotoxic insecticides is well established in aquatic invertebrates of temperate areas, little is known about organisms from polar regions including Antarctica. Cholinesterase activity was investigated in specimens of the Antarctic scallop, Adamussium colbecki, collected in winter 2000 at Campo Icaro (Ross Sea, Antarctica) for preliminary characterization of a potentially new biomarker. Characterization of various ChE enzymes using specific substrates including an acetylthiocholine iodide (ASCh) and a butyrylthiocholine iodide (BSCh) was performed in gills, digestive gland and adductor muscle of the scallop. The effect of in vivo Zn2þ exposure in gills and digestive gland of A. colbecki was also studied. All the tissues expressed ChE activity (gill>adductor muscle>digestive gland) in accordance with data reported for marine mussels (Mytilus sp.) from temperate areas (1.1–13.8 nmol min1 mg protein1). Significant BSCh-dependent ChE inhibition was also measured with a specific inhibitor, Iso-OMPA. Exposure to Zn2þ does not seem to affect ChE activity in the scallop although some slight differences were observed in substrate specificities (ASCh and BSCh) between treated and untreated organisms. This preliminary study stresses the need for further investigation on ChE activity in A. colbecki as a biomarker for monitoring water contamination in the marine Antarctic environment. Article in Journal/Newspaper Antarc* Antarctic Antarctica Ross Sea Università degli Studi di Genova: CINECA IRIS Antarctic The Antarctic Ross Sea Terra Nova Bay |
institution |
Open Polar |
collection |
Università degli Studi di Genova: CINECA IRIS |
op_collection_id |
ftunivgenova |
language |
English |
topic |
Adamussium colbecki Cholinesterase Antarctica Biomarker |
spellingShingle |
Adamussium colbecki Cholinesterase Antarctica Biomarker CORSI I. S. BONACCI G. SANTOVITO L. CASTAGNOLO S. FOCARDI CHIANTORE, MARIACHIARA Preliminary investigation on cholinesterase activity in Adamussium colbecki from Terra Nova Bay: field and laboratory study |
topic_facet |
Adamussium colbecki Cholinesterase Antarctica Biomarker |
description |
While the inhibition of cholinesterase (ChE) activity as a biomarker of exposure to neurotoxic insecticides is well established in aquatic invertebrates of temperate areas, little is known about organisms from polar regions including Antarctica. Cholinesterase activity was investigated in specimens of the Antarctic scallop, Adamussium colbecki, collected in winter 2000 at Campo Icaro (Ross Sea, Antarctica) for preliminary characterization of a potentially new biomarker. Characterization of various ChE enzymes using specific substrates including an acetylthiocholine iodide (ASCh) and a butyrylthiocholine iodide (BSCh) was performed in gills, digestive gland and adductor muscle of the scallop. The effect of in vivo Zn2þ exposure in gills and digestive gland of A. colbecki was also studied. All the tissues expressed ChE activity (gill>adductor muscle>digestive gland) in accordance with data reported for marine mussels (Mytilus sp.) from temperate areas (1.1–13.8 nmol min1 mg protein1). Significant BSCh-dependent ChE inhibition was also measured with a specific inhibitor, Iso-OMPA. Exposure to Zn2þ does not seem to affect ChE activity in the scallop although some slight differences were observed in substrate specificities (ASCh and BSCh) between treated and untreated organisms. This preliminary study stresses the need for further investigation on ChE activity in A. colbecki as a biomarker for monitoring water contamination in the marine Antarctic environment. |
author2 |
Corsi, I. S., Bonacci G., Santovito Chiantore, Mariachiara L., Castagnolo S., Focardi |
format |
Article in Journal/Newspaper |
author |
CORSI I. S. BONACCI G. SANTOVITO L. CASTAGNOLO S. FOCARDI CHIANTORE, MARIACHIARA |
author_facet |
CORSI I. S. BONACCI G. SANTOVITO L. CASTAGNOLO S. FOCARDI CHIANTORE, MARIACHIARA |
author_sort |
CORSI I. |
title |
Preliminary investigation on cholinesterase activity in Adamussium colbecki from Terra Nova Bay: field and laboratory study |
title_short |
Preliminary investigation on cholinesterase activity in Adamussium colbecki from Terra Nova Bay: field and laboratory study |
title_full |
Preliminary investigation on cholinesterase activity in Adamussium colbecki from Terra Nova Bay: field and laboratory study |
title_fullStr |
Preliminary investigation on cholinesterase activity in Adamussium colbecki from Terra Nova Bay: field and laboratory study |
title_full_unstemmed |
Preliminary investigation on cholinesterase activity in Adamussium colbecki from Terra Nova Bay: field and laboratory study |
title_sort |
preliminary investigation on cholinesterase activity in adamussium colbecki from terra nova bay: field and laboratory study |
publisher |
Editore Gordon and Breach Science Publishers Taylor & Francis Limited:Rankine Road, Basingstoke RG24 8PR United Kingdom:011 44 1256 813035, EMAIL: madeline.sims@tandf.co.uk, info@tandf.co.uk, INTERNET: http://www.tandf.co.uk, Fax: 011 44 1256 330245 |
publishDate |
2004 |
url |
http://hdl.handle.net/11567/213053 |
geographic |
Antarctic The Antarctic Ross Sea Terra Nova Bay |
geographic_facet |
Antarctic The Antarctic Ross Sea Terra Nova Bay |
genre |
Antarc* Antarctic Antarctica Ross Sea |
genre_facet |
Antarc* Antarctic Antarctica Ross Sea |
op_relation |
volume:20 (Suppl 1) firstpage:79 lastpage:87 journal:CHEMISTRY IN ECOLOGY http://hdl.handle.net/11567/213053 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-5044252695 |
_version_ |
1790607145478127616 |