Dynamics of the Pacific oyster pathobiota during mortality episodes in Europe assessed by 16S rRNA gene profiling and a new target enrichment next-generation sequencing strategy
Infectious agents such as the bacteria Vibrio aestuarianus or Ostreid herpesvirus 1 have been repeatedly associated with dramatic disease outbreaks of Crassostrea gigas beds in Europe. Beside roles played by these pathogens, microbial infections in C. gigas may derive from the contribution of a larg...
Published in: | Environmental Microbiology |
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Format: | Article in Journal/Newspaper |
Language: | English |
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Blackwell Publishing Ltd
2019
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Online Access: | https://hdl.handle.net/11567/1002282 https://doi.org/10.1111/1462-2920.14750 |
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ftunivgenova:oai:iris.unige.it:11567/1002282 2024-04-14T08:10:43+00:00 Dynamics of the Pacific oyster pathobiota during mortality episodes in Europe assessed by 16S rRNA gene profiling and a new target enrichment next-generation sequencing strategy Lasa A. di Cesare A. Tassistro G. Borello A. Gualdi S. Furones D. Carrasco N. Cheslett D. Brechon A. Paillard C. Bidault A. Pernet F. Canesi L. Edomi P. Pallavicini A. Pruzzo C. Vezzulli L. Lasa, A. di Cesare, A. Tassistro, G. Borello, A. Gualdi, S. Furones, D. Carrasco, N. Cheslett, D. Brechon, A. Paillard, C. Bidault, A. Pernet, F. Canesi, L. Edomi, P. Pallavicini, A. Pruzzo, C. Vezzulli, L. 2019 STAMPA https://hdl.handle.net/11567/1002282 https://doi.org/10.1111/1462-2920.14750 eng eng Blackwell Publishing Ltd place:Oxford info:eu-repo/semantics/altIdentifier/pmid/31325353 info:eu-repo/semantics/altIdentifier/wos/WOS:000481006000001 volume:21 (12) firstpage:4548 lastpage:4562 numberofpages:15 journal:ENVIRONMENTAL MICROBIOLOGY https://hdl.handle.net/11567/1002282 doi:10.1111/1462-2920.14750 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85070071463 info:eu-repo/semantics/article 2019 ftunivgenova https://doi.org/10.1111/1462-2920.14750 2024-03-21T02:33:45Z Infectious agents such as the bacteria Vibrio aestuarianus or Ostreid herpesvirus 1 have been repeatedly associated with dramatic disease outbreaks of Crassostrea gigas beds in Europe. Beside roles played by these pathogens, microbial infections in C. gigas may derive from the contribution of a larger number of microorganisms than previously thought, according to an emerging view supporting the polymicrobial nature of bivalve diseases. In this study, the microbial communities associated with a large number of C. gigas samples collected during recurrent mortality episodes at different European sites were investigated by real-time PCR and 16SrRNA gene-based microbial profiling. A new target enrichment next-generation sequencing protocol for selective capturing of 884 phylogenetic and virulence markers of the potential microbial pathogenic community in oyster tissue was developed allowing high taxonomic resolution analysis of the bivalve pathobiota. Comparative analysis of contrasting C. gigas samples conducted using these methods revealed that oyster experiencing mortality outbreaks displayed signs of microbiota disruption associated with the presence of previously undetected potential pathogenic microbial species mostly belonging to genus Vibrio and Arcobacter. The role of these species and their consortia should be targeted by future studies aiming to shed light on mechanisms underlying polymicrobial infections in C. gigas. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Università degli Studi di Genova: CINECA IRIS Pacific Environmental Microbiology 21 12 4548 4562 |
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Open Polar |
collection |
Università degli Studi di Genova: CINECA IRIS |
op_collection_id |
ftunivgenova |
language |
English |
description |
Infectious agents such as the bacteria Vibrio aestuarianus or Ostreid herpesvirus 1 have been repeatedly associated with dramatic disease outbreaks of Crassostrea gigas beds in Europe. Beside roles played by these pathogens, microbial infections in C. gigas may derive from the contribution of a larger number of microorganisms than previously thought, according to an emerging view supporting the polymicrobial nature of bivalve diseases. In this study, the microbial communities associated with a large number of C. gigas samples collected during recurrent mortality episodes at different European sites were investigated by real-time PCR and 16SrRNA gene-based microbial profiling. A new target enrichment next-generation sequencing protocol for selective capturing of 884 phylogenetic and virulence markers of the potential microbial pathogenic community in oyster tissue was developed allowing high taxonomic resolution analysis of the bivalve pathobiota. Comparative analysis of contrasting C. gigas samples conducted using these methods revealed that oyster experiencing mortality outbreaks displayed signs of microbiota disruption associated with the presence of previously undetected potential pathogenic microbial species mostly belonging to genus Vibrio and Arcobacter. The role of these species and their consortia should be targeted by future studies aiming to shed light on mechanisms underlying polymicrobial infections in C. gigas. |
author2 |
Lasa, A. di Cesare, A. Tassistro, G. Borello, A. Gualdi, S. Furones, D. Carrasco, N. Cheslett, D. Brechon, A. Paillard, C. Bidault, A. Pernet, F. Canesi, L. Edomi, P. Pallavicini, A. Pruzzo, C. Vezzulli, L. |
format |
Article in Journal/Newspaper |
author |
Lasa A. di Cesare A. Tassistro G. Borello A. Gualdi S. Furones D. Carrasco N. Cheslett D. Brechon A. Paillard C. Bidault A. Pernet F. Canesi L. Edomi P. Pallavicini A. Pruzzo C. Vezzulli L. |
spellingShingle |
Lasa A. di Cesare A. Tassistro G. Borello A. Gualdi S. Furones D. Carrasco N. Cheslett D. Brechon A. Paillard C. Bidault A. Pernet F. Canesi L. Edomi P. Pallavicini A. Pruzzo C. Vezzulli L. Dynamics of the Pacific oyster pathobiota during mortality episodes in Europe assessed by 16S rRNA gene profiling and a new target enrichment next-generation sequencing strategy |
author_facet |
Lasa A. di Cesare A. Tassistro G. Borello A. Gualdi S. Furones D. Carrasco N. Cheslett D. Brechon A. Paillard C. Bidault A. Pernet F. Canesi L. Edomi P. Pallavicini A. Pruzzo C. Vezzulli L. |
author_sort |
Lasa A. |
title |
Dynamics of the Pacific oyster pathobiota during mortality episodes in Europe assessed by 16S rRNA gene profiling and a new target enrichment next-generation sequencing strategy |
title_short |
Dynamics of the Pacific oyster pathobiota during mortality episodes in Europe assessed by 16S rRNA gene profiling and a new target enrichment next-generation sequencing strategy |
title_full |
Dynamics of the Pacific oyster pathobiota during mortality episodes in Europe assessed by 16S rRNA gene profiling and a new target enrichment next-generation sequencing strategy |
title_fullStr |
Dynamics of the Pacific oyster pathobiota during mortality episodes in Europe assessed by 16S rRNA gene profiling and a new target enrichment next-generation sequencing strategy |
title_full_unstemmed |
Dynamics of the Pacific oyster pathobiota during mortality episodes in Europe assessed by 16S rRNA gene profiling and a new target enrichment next-generation sequencing strategy |
title_sort |
dynamics of the pacific oyster pathobiota during mortality episodes in europe assessed by 16s rrna gene profiling and a new target enrichment next-generation sequencing strategy |
publisher |
Blackwell Publishing Ltd |
publishDate |
2019 |
url |
https://hdl.handle.net/11567/1002282 https://doi.org/10.1111/1462-2920.14750 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_relation |
info:eu-repo/semantics/altIdentifier/pmid/31325353 info:eu-repo/semantics/altIdentifier/wos/WOS:000481006000001 volume:21 (12) firstpage:4548 lastpage:4562 numberofpages:15 journal:ENVIRONMENTAL MICROBIOLOGY https://hdl.handle.net/11567/1002282 doi:10.1111/1462-2920.14750 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85070071463 |
op_doi |
https://doi.org/10.1111/1462-2920.14750 |
container_title |
Environmental Microbiology |
container_volume |
21 |
container_issue |
12 |
container_start_page |
4548 |
op_container_end_page |
4562 |
_version_ |
1796308368024928256 |