Recombinant IFN-γ from the bank vole Myodes glareolus: a novel tool for research on rodent reservoirs of zoonotic pathogens
Rodent species like Myodes glareolus and Microtus spp. are natural reservoirs for many zoonotic pathogens causing human diseases and are gaining increasing interest in the field of eco-immunology as candidate animal models. Despite their importance the lack of immunological reagents has hampered res...
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ftunivfreiburg:oai:freidok.uni-freiburg.de:143906 2023-05-15T17:12:35+02:00 Recombinant IFN-γ from the bank vole Myodes glareolus: a novel tool for research on rodent reservoirs of zoonotic pathogens Torelli, Francesca Zander, Steffen Ellerbrok, Heinz Kochs, Georg Ulrich, Rainer G. Klotz, Christian Seeber, Frank 2018 pdf https://freidok.uni-freiburg.de/data/143906 https://nbn-resolving.org/urn:nbn:de:bsz:25-freidok-1439067 https://doi.org/10.1038/s41598-018-21143-0 https://freidok.uni-freiburg.de/dnb/download/143906 eng eng https://freidok.uni-freiburg.de/data/143906 free Scientific reports. - 8, 1 (2018) , 2797, ISSN: 2045-2322 article 2018 ftunivfreiburg https://doi.org/10.1038/s41598-018-21143-0 2022-11-29T21:01:26Z Rodent species like Myodes glareolus and Microtus spp. are natural reservoirs for many zoonotic pathogens causing human diseases and are gaining increasing interest in the field of eco-immunology as candidate animal models. Despite their importance the lack of immunological reagents has hampered research in these animal species. Here we report the recombinant production and functional characterization of IFN-γ, a central mediator of host’s innate and adaptive immune responses, from the bank vole M. glareolus. Soluble dimeric recMgIFN-γ was purified in high yield from Escherichia coli. Its activity on M. glareolus and Microtus arvalis kidney cell lines was assessed by immunofluorescent detection of nuclear translocation and phosphorylation of the transcription factor STAT1. RecMgIFN-γ also induced expression of an IFN-γ-regulated innate immunity gene. Inhibition of vesicular stomatitis virus replication in vole cells upon recMgIFN-γ treatment provided further evidence of its biological activity. Finally, we established a recMgIFN-γ-responsive bank vole reporter cell line that allows the sensitive titration of the cytokine activity via a bioluminescence reporter assay. Taken together, we report valuable tools for future investigations on the immune response against zoonotic pathogens in their natural animal hosts, which might foster the development of novel animal models. Article in Journal/Newspaper Microtus arvalis University of Freiburg: FreiDok Scientific Reports 8 1 |
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Open Polar |
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University of Freiburg: FreiDok |
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ftunivfreiburg |
language |
English |
description |
Rodent species like Myodes glareolus and Microtus spp. are natural reservoirs for many zoonotic pathogens causing human diseases and are gaining increasing interest in the field of eco-immunology as candidate animal models. Despite their importance the lack of immunological reagents has hampered research in these animal species. Here we report the recombinant production and functional characterization of IFN-γ, a central mediator of host’s innate and adaptive immune responses, from the bank vole M. glareolus. Soluble dimeric recMgIFN-γ was purified in high yield from Escherichia coli. Its activity on M. glareolus and Microtus arvalis kidney cell lines was assessed by immunofluorescent detection of nuclear translocation and phosphorylation of the transcription factor STAT1. RecMgIFN-γ also induced expression of an IFN-γ-regulated innate immunity gene. Inhibition of vesicular stomatitis virus replication in vole cells upon recMgIFN-γ treatment provided further evidence of its biological activity. Finally, we established a recMgIFN-γ-responsive bank vole reporter cell line that allows the sensitive titration of the cytokine activity via a bioluminescence reporter assay. Taken together, we report valuable tools for future investigations on the immune response against zoonotic pathogens in their natural animal hosts, which might foster the development of novel animal models. |
format |
Article in Journal/Newspaper |
author |
Torelli, Francesca Zander, Steffen Ellerbrok, Heinz Kochs, Georg Ulrich, Rainer G. Klotz, Christian Seeber, Frank |
spellingShingle |
Torelli, Francesca Zander, Steffen Ellerbrok, Heinz Kochs, Georg Ulrich, Rainer G. Klotz, Christian Seeber, Frank Recombinant IFN-γ from the bank vole Myodes glareolus: a novel tool for research on rodent reservoirs of zoonotic pathogens |
author_facet |
Torelli, Francesca Zander, Steffen Ellerbrok, Heinz Kochs, Georg Ulrich, Rainer G. Klotz, Christian Seeber, Frank |
author_sort |
Torelli, Francesca |
title |
Recombinant IFN-γ from the bank vole Myodes glareolus: a novel tool for research on rodent reservoirs of zoonotic pathogens |
title_short |
Recombinant IFN-γ from the bank vole Myodes glareolus: a novel tool for research on rodent reservoirs of zoonotic pathogens |
title_full |
Recombinant IFN-γ from the bank vole Myodes glareolus: a novel tool for research on rodent reservoirs of zoonotic pathogens |
title_fullStr |
Recombinant IFN-γ from the bank vole Myodes glareolus: a novel tool for research on rodent reservoirs of zoonotic pathogens |
title_full_unstemmed |
Recombinant IFN-γ from the bank vole Myodes glareolus: a novel tool for research on rodent reservoirs of zoonotic pathogens |
title_sort |
recombinant ifn-γ from the bank vole myodes glareolus: a novel tool for research on rodent reservoirs of zoonotic pathogens |
publishDate |
2018 |
url |
https://freidok.uni-freiburg.de/data/143906 https://nbn-resolving.org/urn:nbn:de:bsz:25-freidok-1439067 https://doi.org/10.1038/s41598-018-21143-0 https://freidok.uni-freiburg.de/dnb/download/143906 |
genre |
Microtus arvalis |
genre_facet |
Microtus arvalis |
op_source |
Scientific reports. - 8, 1 (2018) , 2797, ISSN: 2045-2322 |
op_relation |
https://freidok.uni-freiburg.de/data/143906 |
op_rights |
free |
op_doi |
https://doi.org/10.1038/s41598-018-21143-0 |
container_title |
Scientific Reports |
container_volume |
8 |
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1 |
_version_ |
1766069372105785344 |