Microbial consortium associated with the antarctic marine ciliate Euplotes focardii: an investigation from genomic sequences
We report the characterization of the bacterial consortium associated to Euplotes focardii, a strictly psychrophilic marine ciliate that was maintained in laboratory cultures at 4 °C after its first isolation from Terra Nova Bay, in Antarctica. By Illumina genome analyser, we obtained 11,179 contigs...
Published in: | Microbial Ecology |
---|---|
Main Authors: | , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
2015
|
Subjects: | |
Online Access: | https://freidok.uni-freiburg.de/data/123278 https://nbn-resolving.org/urn:nbn:de:bsz:25-freidok-1232789 https://doi.org/10.1007/s00248-015-0568-9 https://freidok.uni-freiburg.de/dnb/download/123278 |
id |
ftunivfreiburg:oai:freidok.uni-freiburg.de:123278 |
---|---|
record_format |
openpolar |
spelling |
ftunivfreiburg:oai:freidok.uni-freiburg.de:123278 2023-05-15T13:45:47+02:00 Microbial consortium associated with the antarctic marine ciliate Euplotes focardii: an investigation from genomic sequences Pucciarelli, Sandra Devaraj, Raghul Rajan Mancini, Alessio Ballarini, Patrizia Castelli, Michele Schrallhammer, Martina Petroni, Giulio Miceli, Cristina 2015 pdf https://freidok.uni-freiburg.de/data/123278 https://nbn-resolving.org/urn:nbn:de:bsz:25-freidok-1232789 https://doi.org/10.1007/s00248-015-0568-9 https://freidok.uni-freiburg.de/dnb/download/123278 eng eng https://freidok.uni-freiburg.de/data/123278 free Microbial ecology. - 70, 2 (2015) , 484-497, ISSN: 0095-3628 article 2015 ftunivfreiburg https://doi.org/10.1007/s00248-015-0568-9 2023-02-05T23:48:29Z We report the characterization of the bacterial consortium associated to Euplotes focardii, a strictly psychrophilic marine ciliate that was maintained in laboratory cultures at 4 °C after its first isolation from Terra Nova Bay, in Antarctica. By Illumina genome analyser, we obtained 11,179 contigs of potential prokaryotic origin and classified them according to the NCBI’s prokaryotic attributes table. The majority of these sequences correspond to either Bacteroidetes (16 %) or Proteobacteria (78 %). The latter were dominated by gamma- (39 %, including sequences related to the pathogenic genus Francisella), and alpha-proteobacterial (30 %) sequences. Analysis of the Pfam domain family and Gene Ontology term variation revealed that the most frequent terms that appear unique to this consortium correspond to proteins involved in “transmembrane transporter activity” and “oxidoreductase activity”. Furthermore, we identified genes that encode for enzymes involved in the catabolism of complex substance for energy reserves. We also characterized members of the transposase and integrase superfamilies, whose role in bacterial evolution is well documented, as well as putative antifreeze proteins. Antibiotic treatments of E. focardii cultures delayed the cell division of the ciliate. To conclude, our results indicate that this consortium is largely represented by bacteria derived from the original Antarctic sample and may contribute to the survival of E. focardii in laboratory condition. Furthermore, our results suggest that these bacteria may have a more general role in E. focardii survival in its natural cold and oxidative environment. Article in Journal/Newspaper Antarc* Antarctic Antarctica University of Freiburg: FreiDok Antarctic Terra Nova Bay The Antarctic Microbial Ecology 70 2 484 497 |
institution |
Open Polar |
collection |
University of Freiburg: FreiDok |
op_collection_id |
ftunivfreiburg |
language |
English |
description |
We report the characterization of the bacterial consortium associated to Euplotes focardii, a strictly psychrophilic marine ciliate that was maintained in laboratory cultures at 4 °C after its first isolation from Terra Nova Bay, in Antarctica. By Illumina genome analyser, we obtained 11,179 contigs of potential prokaryotic origin and classified them according to the NCBI’s prokaryotic attributes table. The majority of these sequences correspond to either Bacteroidetes (16 %) or Proteobacteria (78 %). The latter were dominated by gamma- (39 %, including sequences related to the pathogenic genus Francisella), and alpha-proteobacterial (30 %) sequences. Analysis of the Pfam domain family and Gene Ontology term variation revealed that the most frequent terms that appear unique to this consortium correspond to proteins involved in “transmembrane transporter activity” and “oxidoreductase activity”. Furthermore, we identified genes that encode for enzymes involved in the catabolism of complex substance for energy reserves. We also characterized members of the transposase and integrase superfamilies, whose role in bacterial evolution is well documented, as well as putative antifreeze proteins. Antibiotic treatments of E. focardii cultures delayed the cell division of the ciliate. To conclude, our results indicate that this consortium is largely represented by bacteria derived from the original Antarctic sample and may contribute to the survival of E. focardii in laboratory condition. Furthermore, our results suggest that these bacteria may have a more general role in E. focardii survival in its natural cold and oxidative environment. |
format |
Article in Journal/Newspaper |
author |
Pucciarelli, Sandra Devaraj, Raghul Rajan Mancini, Alessio Ballarini, Patrizia Castelli, Michele Schrallhammer, Martina Petroni, Giulio Miceli, Cristina |
spellingShingle |
Pucciarelli, Sandra Devaraj, Raghul Rajan Mancini, Alessio Ballarini, Patrizia Castelli, Michele Schrallhammer, Martina Petroni, Giulio Miceli, Cristina Microbial consortium associated with the antarctic marine ciliate Euplotes focardii: an investigation from genomic sequences |
author_facet |
Pucciarelli, Sandra Devaraj, Raghul Rajan Mancini, Alessio Ballarini, Patrizia Castelli, Michele Schrallhammer, Martina Petroni, Giulio Miceli, Cristina |
author_sort |
Pucciarelli, Sandra |
title |
Microbial consortium associated with the antarctic marine ciliate Euplotes focardii: an investigation from genomic sequences |
title_short |
Microbial consortium associated with the antarctic marine ciliate Euplotes focardii: an investigation from genomic sequences |
title_full |
Microbial consortium associated with the antarctic marine ciliate Euplotes focardii: an investigation from genomic sequences |
title_fullStr |
Microbial consortium associated with the antarctic marine ciliate Euplotes focardii: an investigation from genomic sequences |
title_full_unstemmed |
Microbial consortium associated with the antarctic marine ciliate Euplotes focardii: an investigation from genomic sequences |
title_sort |
microbial consortium associated with the antarctic marine ciliate euplotes focardii: an investigation from genomic sequences |
publishDate |
2015 |
url |
https://freidok.uni-freiburg.de/data/123278 https://nbn-resolving.org/urn:nbn:de:bsz:25-freidok-1232789 https://doi.org/10.1007/s00248-015-0568-9 https://freidok.uni-freiburg.de/dnb/download/123278 |
geographic |
Antarctic Terra Nova Bay The Antarctic |
geographic_facet |
Antarctic Terra Nova Bay The Antarctic |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_source |
Microbial ecology. - 70, 2 (2015) , 484-497, ISSN: 0095-3628 |
op_relation |
https://freidok.uni-freiburg.de/data/123278 |
op_rights |
free |
op_doi |
https://doi.org/10.1007/s00248-015-0568-9 |
container_title |
Microbial Ecology |
container_volume |
70 |
container_issue |
2 |
container_start_page |
484 |
op_container_end_page |
497 |
_version_ |
1766230907574812672 |