Summary: | A, C, E, G) The normalized counts for the circadian series under light-dark of medium intensity of white light (LDM) conditions plotted as standard deviation. B, D, F, H) Boxplots of all light conditions at 18:00 and 02:00 at 690 dd, where outliers are indicated by red asterisks and the median is shown as a line inside each box. A and C) The two nr1d4b genes showed a similar expression pattern through circadian sampling. E and G) The genes arntl2b and arntl2c showed a similar trend but only arntl2b showed a decrease in expression at dark phase (02:00). B and D) For both nr1d4b genes, expression was lower at 02:00 compared to 18:00 in LDM and light-dark of red (LDR). Statistically significant differences were confirmed by conducting Wald test in DESeq2, where downregulation was significant in LDM for both genes and in LDR for nr1d4b . 1 . F and H) Validated by DESeq2, arntl2c was significantly upregulated during light phase (18:00) in all light conditions compared to continuous darkness (DD).
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