Experimental setup and spectral properties of the light-emitting diodes (LED) arrays.

• Main Authors: Mariann Eilertsen (11502317), David W. P. Dolan (14247792), Charlotte M. Bolton (11780345), Rita Karlsen (13025421), Wayne I. L. Davies (8028890), Rolf B. Edvardsen (6913946), Tomasz Furmanek (283506), Harald Sveier (8218770), Herve Migaud (308426), Jon Vidar Helvik (8028887)
• Format: Still Image
• Language: unknown
• Published: 2022
• Subjects: Microbiology; Cell Biology; Genetics; Molecular Biology; Neuroscience; Physiology; Ecology; Developmental Biology; Inorganic Chemistry; Mental Health; Environmental Sciences not elsewhere classified; Biological Sciences not elsewhere classified; salmo salar </; atlantic salmon (<; complex photoreceptive system; photoperiod </ p; div >< p; early developmental stages; two clock genes; several clock genes; differing light conditions; developing teleost prior; circadian profile showed; different light conditions; constant white light; constant conditions; circadian profile; clock genes; white light; light conditions; constant light; significantly different; results showed; present prior; photoreceptive capacity; developmental series; expressed early; early development; light environment; light condition; green light; many genes; genes related; genes associated; transcriptomic response; transcriptome profile; study implies; significantly cycling; seasonal changes; poorly differentiated; Atlantic salmon; Salmo salar
• Online Access: https://doi.org/10.1371/journal.pgen.1010529.s001

A) Schematic illustration of the experimental setup showing constant or changing light environments. Embryos and alevins were exposed to different lighting regimes from fertilization to first feeding (121 days). The experiments were divided across three parameters: (i) different photoperiods (i.e. continuous white light (LL), continuous darkness (DD), or a white light:dark (LD) cycle of 14:10); (ii) intensities of LD white light, consisting of high (LDH), medium (LDM) or low (LDL) light levels; (iii) a LD cycling condition with medium intensity of light of different wavelengths of the visible spectrum, namely (blue (LDB), green (LDG), red (LDR). The arrows indicate sampling points during development, 255 dd (40 days), 379 dd (60 days), 555 dd (90 days) and 690 dd (113 days). The circadian sampling for LDM at 690 dd is highlighted by wider boxes indicating the sampling points for the 24 h series. All light regimes were sampled at 18:00 and 02:00. B) Spectrum of the white LED, warm white 2700K (λ max = ~610 nm), at medium intensity and the narrow bandwidth spectrum of blue (λ max = ~450nm), green (λ max = ~535nm), and red (λ max = ~660nm) LEDs. C) Pictures of eggs in the egg incubators with medium white, blue, green, and red light. (TIF)