| Summary: | A) Schematic illustration of the experimental setup showing constant or changing light environments. Embryos and alevins were exposed to different lighting regimes from fertilization to first feeding (121 days). The experiments were divided across three parameters: (i) different photoperiods (i.e. continuous white light (LL), continuous darkness (DD), or a white light:dark (LD) cycle of 14:10); (ii) intensities of LD white light, consisting of high (LDH), medium (LDM) or low (LDL) light levels; (iii) a LD cycling condition with medium intensity of light of different wavelengths of the visible spectrum, namely (blue (LDB), green (LDG), red (LDR). The arrows indicate sampling points during development, 255 dd (40 days), 379 dd (60 days), 555 dd (90 days) and 690 dd (113 days). The circadian sampling for LDM at 690 dd is highlighted by wider boxes indicating the sampling points for the 24 h series. All light regimes were sampled at 18:00 and 02:00. B) Spectrum of the white LED, warm white 2700K (λ max = ~610 nm), at medium intensity and the narrow bandwidth spectrum of blue (λ max = ~450nm), green (λ max = ~535nm), and red (λ max = ~660nm) LEDs. C) Pictures of eggs in the egg incubators with medium white, blue, green, and red light. (TIF)
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