Molecular detection of anaplasmataceae agents on dogs from the departament of Piura, Peru
This study aimed to study the prevalence of Anaplasmataceae organisms through the nested-PCR and phylogenetic analysis on domestic dogs in the Department of Piura, Peru. Two hundred and twelve canine blood samples were randomly collected on dogs from the central urban areas at the Piura Department i...
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Universidade Federal de Goiás
2022
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ftunivfgoiasojs:oai:ojs.revistas.ufg.br:article/71516 2023-05-15T15:51:14+02:00 Molecular detection of anaplasmataceae agents on dogs from the departament of Piura, Peru Cerro Temoche, Luís Fernando Dias Cordeiro, Matheus S. Seabra-Junior, Eloy Destri Emmerick Campos, Sabrina Pereira Almosny, Nadia Regina 2022-06-23 application/pdf https://revistas.ufg.br/iptsp/article/view/71516 eng eng Universidade Federal de Goiás https://revistas.ufg.br/iptsp/article/view/71516/38544 https://revistas.ufg.br/iptsp/article/view/71516 Copyright (c) 2022 Revista de Patologia Tropical / Journal of Tropical Pathology Revista de Patologia Tropical / Journal of Tropical Pathology; Vol. 51 No. 2 (2022); 134-144 Revista de Patologia Tropical / Journal of Tropical Pathology; v. 51 n. 2 (2022); 134-144 1980-8178 0301-0406 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2022 ftunivfgoiasojs 2022-08-06T23:28:14Z This study aimed to study the prevalence of Anaplasmataceae organisms through the nested-PCR and phylogenetic analysis on domestic dogs in the Department of Piura, Peru. Two hundred and twelve canine blood samples were randomly collected on dogs from the central urban areas at the Piura Department in Peru. The extracted DNAs were tested, by nested-PCR based on 16SrRNA gene, to identify agents from Anaplasmataceae family. These results show that there was a prevalence of 18.5% (40/216) of positive dogs, 13.8% (30/216) for Ehrlichia canis, 7.4% (16/216) for Anaplasma platys and 0.1% (2/216) for Ehrlichia sp. confirmed by sequencing analysis. Co-positivity among Anaplasmataceae family species was present in 25% (10/40) of positive samples. There was a significant association among Anaplasmataceae family infection in dogs and the following variables: sex (p=0.034), presence of ticks (p=0.0001), and socio-economic status (p=0.001). There was no statistical association on the variables “living with other animals” and “age group” (p=0.1074). The partial sequences on the portion of the 16S rRNA gene, from positive samples for agents of Anaplasmataceae family demonstrated an identity of 97-100% with the isolated E. canis and A. platys obtained from the GenBank. This is the first study on infection by agents of Anaplasmataceae family in dogs in the Department of Piura, through molecular analysis.KEY WORDS: Anaplasmosis; Canis lupus; ehrlichiosis; molecular analysis; South America This study aimed to study the prevalence of Anaplasmataceae organisms through the nested-PCR and phylogenetic analysis on domestic dogs in the Department of Piura, Peru. Two hundred and twelve canine blood samples were randomly collected on dogs from the central urban areas at the Piura Department in Peru. The extracted DNAs were tested, by nested-PCR based on 16SrRNA gene, to identify agents from Anaplasmataceae family. These results show that there was a prevalence of 18.5% (40/216) of positive dogs, 13.8% (30/216) for Ehrlichia canis, 7.4% ... Article in Journal/Newspaper Canis lupus Portal de Periódicos da UFG (Universidade Federal de Goiás) |
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Portal de Periódicos da UFG (Universidade Federal de Goiás) |
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ftunivfgoiasojs |
language |
English |
description |
This study aimed to study the prevalence of Anaplasmataceae organisms through the nested-PCR and phylogenetic analysis on domestic dogs in the Department of Piura, Peru. Two hundred and twelve canine blood samples were randomly collected on dogs from the central urban areas at the Piura Department in Peru. The extracted DNAs were tested, by nested-PCR based on 16SrRNA gene, to identify agents from Anaplasmataceae family. These results show that there was a prevalence of 18.5% (40/216) of positive dogs, 13.8% (30/216) for Ehrlichia canis, 7.4% (16/216) for Anaplasma platys and 0.1% (2/216) for Ehrlichia sp. confirmed by sequencing analysis. Co-positivity among Anaplasmataceae family species was present in 25% (10/40) of positive samples. There was a significant association among Anaplasmataceae family infection in dogs and the following variables: sex (p=0.034), presence of ticks (p=0.0001), and socio-economic status (p=0.001). There was no statistical association on the variables “living with other animals” and “age group” (p=0.1074). The partial sequences on the portion of the 16S rRNA gene, from positive samples for agents of Anaplasmataceae family demonstrated an identity of 97-100% with the isolated E. canis and A. platys obtained from the GenBank. This is the first study on infection by agents of Anaplasmataceae family in dogs in the Department of Piura, through molecular analysis.KEY WORDS: Anaplasmosis; Canis lupus; ehrlichiosis; molecular analysis; South America This study aimed to study the prevalence of Anaplasmataceae organisms through the nested-PCR and phylogenetic analysis on domestic dogs in the Department of Piura, Peru. Two hundred and twelve canine blood samples were randomly collected on dogs from the central urban areas at the Piura Department in Peru. The extracted DNAs were tested, by nested-PCR based on 16SrRNA gene, to identify agents from Anaplasmataceae family. These results show that there was a prevalence of 18.5% (40/216) of positive dogs, 13.8% (30/216) for Ehrlichia canis, 7.4% ... |
format |
Article in Journal/Newspaper |
author |
Cerro Temoche, Luís Fernando Dias Cordeiro, Matheus S. Seabra-Junior, Eloy Destri Emmerick Campos, Sabrina Pereira Almosny, Nadia Regina |
spellingShingle |
Cerro Temoche, Luís Fernando Dias Cordeiro, Matheus S. Seabra-Junior, Eloy Destri Emmerick Campos, Sabrina Pereira Almosny, Nadia Regina Molecular detection of anaplasmataceae agents on dogs from the departament of Piura, Peru |
author_facet |
Cerro Temoche, Luís Fernando Dias Cordeiro, Matheus S. Seabra-Junior, Eloy Destri Emmerick Campos, Sabrina Pereira Almosny, Nadia Regina |
author_sort |
Cerro Temoche, Luís Fernando |
title |
Molecular detection of anaplasmataceae agents on dogs from the departament of Piura, Peru |
title_short |
Molecular detection of anaplasmataceae agents on dogs from the departament of Piura, Peru |
title_full |
Molecular detection of anaplasmataceae agents on dogs from the departament of Piura, Peru |
title_fullStr |
Molecular detection of anaplasmataceae agents on dogs from the departament of Piura, Peru |
title_full_unstemmed |
Molecular detection of anaplasmataceae agents on dogs from the departament of Piura, Peru |
title_sort |
molecular detection of anaplasmataceae agents on dogs from the departament of piura, peru |
publisher |
Universidade Federal de Goiás |
publishDate |
2022 |
url |
https://revistas.ufg.br/iptsp/article/view/71516 |
genre |
Canis lupus |
genre_facet |
Canis lupus |
op_source |
Revista de Patologia Tropical / Journal of Tropical Pathology; Vol. 51 No. 2 (2022); 134-144 Revista de Patologia Tropical / Journal of Tropical Pathology; v. 51 n. 2 (2022); 134-144 1980-8178 0301-0406 |
op_relation |
https://revistas.ufg.br/iptsp/article/view/71516/38544 https://revistas.ufg.br/iptsp/article/view/71516 |
op_rights |
Copyright (c) 2022 Revista de Patologia Tropical / Journal of Tropical Pathology |
_version_ |
1766386348822888448 |