Acclimation to darkness in the marine chlorophyte Koliella antarctica cultured under low salinity: hypotheses on its origin in the polar environment.
In order to obtain new insights on the origin and physiology of the marine chlorophyte Koliella antarctica, the response of the microalga was studied at a salinity of 0.2 in the light and during a 60-day dark period. In light conditions, the alga grows and maintains a functional cell organization. I...
Published in: | European Journal of Phycology |
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Online Access: | http://hdl.handle.net/11392/471389 https://doi.org/10.1080/09670260600960850 |
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ftunivferrarair:oai:sfera.unife.it:11392/471389 2024-09-09T19:06:29+00:00 Acclimation to darkness in the marine chlorophyte Koliella antarctica cultured under low salinity: hypotheses on its origin in the polar environment. FERRONI, Lorenzo BALDISSEROTTO, Costanza FASULO, Maria Palmira PANCALDI, Simonetta ZENNARO V SOLDANI C Ferroni, Lorenzo Baldisserotto, Costanza Zennaro, V Soldani, C Fasulo, Maria Palmira Pancaldi, Simonetta 2007 STAMPA http://hdl.handle.net/11392/471389 https://doi.org/10.1080/09670260600960850 eng eng info:eu-repo/semantics/altIdentifier/wos/WOS:000245242200007 volume:42 issue:1 firstpage:91 lastpage:104 journal:EUROPEAN JOURNAL OF PHYCOLOGY http://hdl.handle.net/11392/471389 doi:10.1080/09670260600960850 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-33847191752 usr_valut Antarctica chloroplast darkne Koliella antarctica mitochondrion nucleu photosystem II (PSII) programmed cell death salinity info:eu-repo/semantics/article 2007 ftunivferrarair https://doi.org/10.1080/09670260600960850 2024-06-19T13:27:06Z In order to obtain new insights on the origin and physiology of the marine chlorophyte Koliella antarctica, the response of the microalga was studied at a salinity of 0.2 in the light and during a 60-day dark period. In light conditions, the alga grows and maintains a functional cell organization. In darkness, the chloroplast-to-chromoplast transition, previously described during dark-acclimation in K. antarctica under a salinity of 34 was only partially triggered; thylakoid lamellae became re-organized into short bundles, but storage substructures were almost completely missing. Microspectrofluorometry, pigment analyses, and morphological observations revealed dark-induced degradation of photosystem II (PSII) with relative stability in the light-harvesting complex (LHCII). Chromatin condensation, mitochondrion fragmentation and material digestion in vacuoles were similar to morphological hallmarks of programmed cell death (PCD), but only 30% of cells underwent cell death and, at the end of the experiment, only 1-2% of cells were TUNEL-positive. Rapid recovery in culture growth after exposure to light showed that the changes apparent in the rest of cells were reversible. Taking into account the response of the plastid and assuming an adaptive benefit of PCD, our results are consistent with the hypothesis that K. antarctica evolved from an Antarctic freshwater ancestor. Article in Journal/Newspaper Antarc* Antarctic Antarctica Università degli Studi di Ferrara: CINECA IRIS Antarctic European Journal of Phycology 42 1 91 104 |
institution |
Open Polar |
collection |
Università degli Studi di Ferrara: CINECA IRIS |
op_collection_id |
ftunivferrarair |
language |
English |
topic |
Antarctica chloroplast darkne Koliella antarctica mitochondrion nucleu photosystem II (PSII) programmed cell death salinity |
spellingShingle |
Antarctica chloroplast darkne Koliella antarctica mitochondrion nucleu photosystem II (PSII) programmed cell death salinity FERRONI, Lorenzo BALDISSEROTTO, Costanza FASULO, Maria Palmira PANCALDI, Simonetta ZENNARO V SOLDANI C Acclimation to darkness in the marine chlorophyte Koliella antarctica cultured under low salinity: hypotheses on its origin in the polar environment. |
topic_facet |
Antarctica chloroplast darkne Koliella antarctica mitochondrion nucleu photosystem II (PSII) programmed cell death salinity |
description |
In order to obtain new insights on the origin and physiology of the marine chlorophyte Koliella antarctica, the response of the microalga was studied at a salinity of 0.2 in the light and during a 60-day dark period. In light conditions, the alga grows and maintains a functional cell organization. In darkness, the chloroplast-to-chromoplast transition, previously described during dark-acclimation in K. antarctica under a salinity of 34 was only partially triggered; thylakoid lamellae became re-organized into short bundles, but storage substructures were almost completely missing. Microspectrofluorometry, pigment analyses, and morphological observations revealed dark-induced degradation of photosystem II (PSII) with relative stability in the light-harvesting complex (LHCII). Chromatin condensation, mitochondrion fragmentation and material digestion in vacuoles were similar to morphological hallmarks of programmed cell death (PCD), but only 30% of cells underwent cell death and, at the end of the experiment, only 1-2% of cells were TUNEL-positive. Rapid recovery in culture growth after exposure to light showed that the changes apparent in the rest of cells were reversible. Taking into account the response of the plastid and assuming an adaptive benefit of PCD, our results are consistent with the hypothesis that K. antarctica evolved from an Antarctic freshwater ancestor. |
author2 |
Ferroni, Lorenzo Baldisserotto, Costanza Zennaro, V Soldani, C Fasulo, Maria Palmira Pancaldi, Simonetta |
format |
Article in Journal/Newspaper |
author |
FERRONI, Lorenzo BALDISSEROTTO, Costanza FASULO, Maria Palmira PANCALDI, Simonetta ZENNARO V SOLDANI C |
author_facet |
FERRONI, Lorenzo BALDISSEROTTO, Costanza FASULO, Maria Palmira PANCALDI, Simonetta ZENNARO V SOLDANI C |
author_sort |
FERRONI, Lorenzo |
title |
Acclimation to darkness in the marine chlorophyte Koliella antarctica cultured under low salinity: hypotheses on its origin in the polar environment. |
title_short |
Acclimation to darkness in the marine chlorophyte Koliella antarctica cultured under low salinity: hypotheses on its origin in the polar environment. |
title_full |
Acclimation to darkness in the marine chlorophyte Koliella antarctica cultured under low salinity: hypotheses on its origin in the polar environment. |
title_fullStr |
Acclimation to darkness in the marine chlorophyte Koliella antarctica cultured under low salinity: hypotheses on its origin in the polar environment. |
title_full_unstemmed |
Acclimation to darkness in the marine chlorophyte Koliella antarctica cultured under low salinity: hypotheses on its origin in the polar environment. |
title_sort |
acclimation to darkness in the marine chlorophyte koliella antarctica cultured under low salinity: hypotheses on its origin in the polar environment. |
publishDate |
2007 |
url |
http://hdl.handle.net/11392/471389 https://doi.org/10.1080/09670260600960850 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_relation |
info:eu-repo/semantics/altIdentifier/wos/WOS:000245242200007 volume:42 issue:1 firstpage:91 lastpage:104 journal:EUROPEAN JOURNAL OF PHYCOLOGY http://hdl.handle.net/11392/471389 doi:10.1080/09670260600960850 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-33847191752 usr_valut |
op_doi |
https://doi.org/10.1080/09670260600960850 |
container_title |
European Journal of Phycology |
container_volume |
42 |
container_issue |
1 |
container_start_page |
91 |
op_container_end_page |
104 |
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1809820547568107520 |