Composition and nutritional values of fatty acids in marine organisms by one-step microwave-assisted extraction/derivatization and comprehensive two-dimensional gas chromatography -flame ionization detector
This work reports the characterization of the lipidic fraction of seven species of marine organisms gathered along the shoreline of the Po Delta Park of Emilia-Romagna Region (Italy) and of the north Adriatic Sea. Two species of oysters (Crassostrea gigas and Ostrea edulis), two species of clams (Ch...
Published in: | Journal of Chromatography B |
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2024
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Online Access: | https://hdl.handle.net/11392/2543911 https://doi.org/10.1016/j.jchromb.2024.124074 https://www.sciencedirect.com/science/article/pii/S1570023224000825?via=ihub |
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ftunivferrarair:oai:sfera.unife.it:11392/2543911 2024-06-23T07:52:18+00:00 Composition and nutritional values of fatty acids in marine organisms by one-step microwave-assisted extraction/derivatization and comprehensive two-dimensional gas chromatography -flame ionization detector Ferrara, Donatella Cescon, Mirco Giacoppo, Giulia Costa, Valentina Purcaro, Giorgia Spadafora, Natasha Damiana Cordero, Chiara Pasti, Luisa Cavazzini, Alberto Beccaria, Marco Ferrara, Donatella Cescon, Mirco Giacoppo, Giulia Costa, Valentina Purcaro, Giorgia Spadafora, Natasha Damiana Cordero, Chiara Pasti, Luisa Cavazzini, Alberto Beccaria, Marco 2024 ELETTRONICO https://hdl.handle.net/11392/2543911 https://doi.org/10.1016/j.jchromb.2024.124074 https://www.sciencedirect.com/science/article/pii/S1570023224000825?via=ihub eng eng info:eu-repo/semantics/altIdentifier/pmid/38460448 info:eu-repo/semantics/altIdentifier/wos/WOS:001219770000001 volume:1236 firstpage:1 lastpage:9 numberofpages:9 journal:JOURNAL OF CHROMATOGRAPHY. B https://hdl.handle.net/11392/2543911 doi:10.1016/j.jchromb.2024.124074 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85187200412 https://www.sciencedirect.com/science/article/pii/S1570023224000825?via=ihub info:eu-repo/semantics/article 2024 ftunivferrarair https://doi.org/10.1016/j.jchromb.2024.124074 2024-06-03T23:47:29Z This work reports the characterization of the lipidic fraction of seven species of marine organisms gathered along the shoreline of the Po Delta Park of Emilia-Romagna Region (Italy) and of the north Adriatic Sea. Two species of oysters (Crassostrea gigas and Ostrea edulis), two species of clams (Chamelea gallina and Ruditapes philippinarum), one species of mussel (Mytilus galloprovincialis), one species of macroalgae (Ulva rigida), and one species of spiny dogfish (Squalus acanthias) were analyzed to characterize their fatty acids profile and related nutritional value. The lipid fraction was simultaneously extracted and transesterified into fatty acid methyl esters (FAMEs) by using a recently developed one-step microwave-assisted extraction/derivatization (MAED) method. The obtained FAMEs extract was analyzed by a rapid comprehensive multidimensional gas chromatography (GC × GC) method (30 min). The system was equipped with a reverse set of columns (polar × non-polar) connected through a reversed fill/flush flow modulator. The GC × GC system was coupled with a flame-ionization detector (FID) for both qualitative and quantitative purposes. The MAED- GC × GC-FID methodology was suitable in the context of samples containing high percentages of omega-3 PUFA. A total of 82 FAMEs were tentatively identified using standards, literature data, and the two-dimensional plot location. FAME profiles obtained with the proposed approach were comparable with reference methods (AOCS Ce 2b-11), showing no significant differences. Moreover, to determine the food nutritional value of the samples investigated, the most common nutritional indices (index of atherogenicity, index thrombogenicity, hypocholesterolemic/hypercholesterolemic ratio, health-promoting index, unsaturation index, and the fish lipid quality index) were calculated from FAME profiles. Among the samples investigated, Squalus acanthias presented the best nutritional score, while Ruditapes philippinarum had the worst score in 3 out of 6 indices. Article in Journal/Newspaper Crassostrea gigas spiny dogfish Squalus acanthias Università degli Studi di Ferrara: CINECA IRIS Fid ENVELOPE(-65.939,-65.939,-68.664,-68.664) Journal of Chromatography B 1236 124074 |
institution |
Open Polar |
collection |
Università degli Studi di Ferrara: CINECA IRIS |
op_collection_id |
ftunivferrarair |
language |
English |
description |
This work reports the characterization of the lipidic fraction of seven species of marine organisms gathered along the shoreline of the Po Delta Park of Emilia-Romagna Region (Italy) and of the north Adriatic Sea. Two species of oysters (Crassostrea gigas and Ostrea edulis), two species of clams (Chamelea gallina and Ruditapes philippinarum), one species of mussel (Mytilus galloprovincialis), one species of macroalgae (Ulva rigida), and one species of spiny dogfish (Squalus acanthias) were analyzed to characterize their fatty acids profile and related nutritional value. The lipid fraction was simultaneously extracted and transesterified into fatty acid methyl esters (FAMEs) by using a recently developed one-step microwave-assisted extraction/derivatization (MAED) method. The obtained FAMEs extract was analyzed by a rapid comprehensive multidimensional gas chromatography (GC × GC) method (30 min). The system was equipped with a reverse set of columns (polar × non-polar) connected through a reversed fill/flush flow modulator. The GC × GC system was coupled with a flame-ionization detector (FID) for both qualitative and quantitative purposes. The MAED- GC × GC-FID methodology was suitable in the context of samples containing high percentages of omega-3 PUFA. A total of 82 FAMEs were tentatively identified using standards, literature data, and the two-dimensional plot location. FAME profiles obtained with the proposed approach were comparable with reference methods (AOCS Ce 2b-11), showing no significant differences. Moreover, to determine the food nutritional value of the samples investigated, the most common nutritional indices (index of atherogenicity, index thrombogenicity, hypocholesterolemic/hypercholesterolemic ratio, health-promoting index, unsaturation index, and the fish lipid quality index) were calculated from FAME profiles. Among the samples investigated, Squalus acanthias presented the best nutritional score, while Ruditapes philippinarum had the worst score in 3 out of 6 indices. |
author2 |
Ferrara, Donatella Cescon, Mirco Giacoppo, Giulia Costa, Valentina Purcaro, Giorgia Spadafora, Natasha Damiana Cordero, Chiara Pasti, Luisa Cavazzini, Alberto Beccaria, Marco |
format |
Article in Journal/Newspaper |
author |
Ferrara, Donatella Cescon, Mirco Giacoppo, Giulia Costa, Valentina Purcaro, Giorgia Spadafora, Natasha Damiana Cordero, Chiara Pasti, Luisa Cavazzini, Alberto Beccaria, Marco |
spellingShingle |
Ferrara, Donatella Cescon, Mirco Giacoppo, Giulia Costa, Valentina Purcaro, Giorgia Spadafora, Natasha Damiana Cordero, Chiara Pasti, Luisa Cavazzini, Alberto Beccaria, Marco Composition and nutritional values of fatty acids in marine organisms by one-step microwave-assisted extraction/derivatization and comprehensive two-dimensional gas chromatography -flame ionization detector |
author_facet |
Ferrara, Donatella Cescon, Mirco Giacoppo, Giulia Costa, Valentina Purcaro, Giorgia Spadafora, Natasha Damiana Cordero, Chiara Pasti, Luisa Cavazzini, Alberto Beccaria, Marco |
author_sort |
Ferrara, Donatella |
title |
Composition and nutritional values of fatty acids in marine organisms by one-step microwave-assisted extraction/derivatization and comprehensive two-dimensional gas chromatography -flame ionization detector |
title_short |
Composition and nutritional values of fatty acids in marine organisms by one-step microwave-assisted extraction/derivatization and comprehensive two-dimensional gas chromatography -flame ionization detector |
title_full |
Composition and nutritional values of fatty acids in marine organisms by one-step microwave-assisted extraction/derivatization and comprehensive two-dimensional gas chromatography -flame ionization detector |
title_fullStr |
Composition and nutritional values of fatty acids in marine organisms by one-step microwave-assisted extraction/derivatization and comprehensive two-dimensional gas chromatography -flame ionization detector |
title_full_unstemmed |
Composition and nutritional values of fatty acids in marine organisms by one-step microwave-assisted extraction/derivatization and comprehensive two-dimensional gas chromatography -flame ionization detector |
title_sort |
composition and nutritional values of fatty acids in marine organisms by one-step microwave-assisted extraction/derivatization and comprehensive two-dimensional gas chromatography -flame ionization detector |
publishDate |
2024 |
url |
https://hdl.handle.net/11392/2543911 https://doi.org/10.1016/j.jchromb.2024.124074 https://www.sciencedirect.com/science/article/pii/S1570023224000825?via=ihub |
long_lat |
ENVELOPE(-65.939,-65.939,-68.664,-68.664) |
geographic |
Fid |
geographic_facet |
Fid |
genre |
Crassostrea gigas spiny dogfish Squalus acanthias |
genre_facet |
Crassostrea gigas spiny dogfish Squalus acanthias |
op_relation |
info:eu-repo/semantics/altIdentifier/pmid/38460448 info:eu-repo/semantics/altIdentifier/wos/WOS:001219770000001 volume:1236 firstpage:1 lastpage:9 numberofpages:9 journal:JOURNAL OF CHROMATOGRAPHY. B https://hdl.handle.net/11392/2543911 doi:10.1016/j.jchromb.2024.124074 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85187200412 https://www.sciencedirect.com/science/article/pii/S1570023224000825?via=ihub |
op_doi |
https://doi.org/10.1016/j.jchromb.2024.124074 |
container_title |
Journal of Chromatography B |
container_volume |
1236 |
container_start_page |
124074 |
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1802643573389131776 |