Preservation of DNA in Pleistocene and Holocene bear species samples from Northwestern Iberian Peninsula
In this paper we present 73 short mitochondrial DNA (mtDNA) sequences obtained from 30 brown bear (Ursus arctos) and 56 cave bear (Ursus spelaeus) samples in several cave sites of Northwestern Iberian Peninsula (Galicia and Asturias), of Holocene and Pleistocene ages. The technique used was PCR ampl...
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ftunivferrarair:oai:sfera.unife.it:11392/2418405 2024-09-09T20:12:48+00:00 Preservation of DNA in Pleistocene and Holocene bear species samples from Northwestern Iberian Peninsula Gonzalez-Fortes G. Garcia-Vazquez A. Pinto-Llona A. C. Grandal-D'Anglade A. Gonzalez-Fortes, G. Garcia-Vazquez, A. Pinto-Llona, A. C. Grandal-D'Anglade, A. 2017 ELETTRONICO http://hdl.handle.net/11392/2418405 http://www.iux.es/pdf/cd39_art07.pdf spa spa info:eu-repo/semantics/altIdentifier/pmid/02134497 volume:39 firstpage:129 lastpage:140 numberofpages:12 journal:CUADERNOS DO LABORATORIO XEOLÓXICO DE LAXE http://hdl.handle.net/11392/2418405 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85021236818 http://www.iux.es/pdf/cd39_art07.pdf info:eu-repo/semantics/article 2017 ftunivferrarair 2024-06-19T13:48:49Z In this paper we present 73 short mitochondrial DNA (mtDNA) sequences obtained from 30 brown bear (Ursus arctos) and 56 cave bear (Ursus spelaeus) samples in several cave sites of Northwestern Iberian Peninsula (Galicia and Asturias), of Holocene and Pleistocene ages. The technique used was PCR amplification of small mtDNA fragments and the subsequent Sanger sequencing of PCR products. In total, 6 different haplotypes were obtained for the brown bear and two for the cave bear. The high success rate of PCR amplifications (85[%], 73 positive amplifications out of a total of 86 samples) demonstrates the good conditions of the Sierra do Courel caves for the preservation of DNA in fossil bone remains, even for tens of millennia old. Article in Journal/Newspaper Ursus arctos Università degli Studi di Ferrara: CINECA IRIS |
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Open Polar |
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Università degli Studi di Ferrara: CINECA IRIS |
op_collection_id |
ftunivferrarair |
language |
Spanish |
description |
In this paper we present 73 short mitochondrial DNA (mtDNA) sequences obtained from 30 brown bear (Ursus arctos) and 56 cave bear (Ursus spelaeus) samples in several cave sites of Northwestern Iberian Peninsula (Galicia and Asturias), of Holocene and Pleistocene ages. The technique used was PCR amplification of small mtDNA fragments and the subsequent Sanger sequencing of PCR products. In total, 6 different haplotypes were obtained for the brown bear and two for the cave bear. The high success rate of PCR amplifications (85[%], 73 positive amplifications out of a total of 86 samples) demonstrates the good conditions of the Sierra do Courel caves for the preservation of DNA in fossil bone remains, even for tens of millennia old. |
author2 |
Gonzalez-Fortes, G. Garcia-Vazquez, A. Pinto-Llona, A. C. Grandal-D'Anglade, A. |
format |
Article in Journal/Newspaper |
author |
Gonzalez-Fortes G. Garcia-Vazquez A. Pinto-Llona A. C. Grandal-D'Anglade A. |
spellingShingle |
Gonzalez-Fortes G. Garcia-Vazquez A. Pinto-Llona A. C. Grandal-D'Anglade A. Preservation of DNA in Pleistocene and Holocene bear species samples from Northwestern Iberian Peninsula |
author_facet |
Gonzalez-Fortes G. Garcia-Vazquez A. Pinto-Llona A. C. Grandal-D'Anglade A. |
author_sort |
Gonzalez-Fortes G. |
title |
Preservation of DNA in Pleistocene and Holocene bear species samples from Northwestern Iberian Peninsula |
title_short |
Preservation of DNA in Pleistocene and Holocene bear species samples from Northwestern Iberian Peninsula |
title_full |
Preservation of DNA in Pleistocene and Holocene bear species samples from Northwestern Iberian Peninsula |
title_fullStr |
Preservation of DNA in Pleistocene and Holocene bear species samples from Northwestern Iberian Peninsula |
title_full_unstemmed |
Preservation of DNA in Pleistocene and Holocene bear species samples from Northwestern Iberian Peninsula |
title_sort |
preservation of dna in pleistocene and holocene bear species samples from northwestern iberian peninsula |
publishDate |
2017 |
url |
http://hdl.handle.net/11392/2418405 http://www.iux.es/pdf/cd39_art07.pdf |
genre |
Ursus arctos |
genre_facet |
Ursus arctos |
op_relation |
info:eu-repo/semantics/altIdentifier/pmid/02134497 volume:39 firstpage:129 lastpage:140 numberofpages:12 journal:CUADERNOS DO LABORATORIO XEOLÓXICO DE LAXE http://hdl.handle.net/11392/2418405 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85021236818 http://www.iux.es/pdf/cd39_art07.pdf |
_version_ |
1809947417850675200 |