A cDNA Microarray for Crassostrea virginica and C. gigas
The eastern oyster, Crassostrea virginica, and the Pacific oyster, C. gigas, are species of global economic significance as well as important components of estuarine ecosystems and models for genetic and environmental studies. To enhance the molecular tools available for oyster research, an internat...
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2007
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Online Access: | http://hdl.handle.net/11392/1411407 https://doi.org/10.1007/s10126-007-9041-1 |
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ftunivferrarair:oai:sfera.unife.it:11392/1411407 2024-09-09T20:02:28+00:00 A cDNA Microarray for Crassostrea virginica and C. gigas Jenny M. J. Chapman R. W. Chen Y. A. McKillen D. J. Trent H. F. Lang P. Escoubas J. Bachere E. Buolo V. Liu Z. J. Gross P. S. Cunningham C. Cupit P. M. Tanguy A. Guo X. Moraga D. Boutet I. Huvet A. De Guise S. Almeida J. S. Warr G. W. MANCIA, Annalaura Jenny, M. J. Chapman, R. W. Mancia, Annalaura Chen, Y. A. Mckillen, D. J. Trent, H. F. Lang, P. Escoubas, J. Bachere, E. Buolo, V. Liu, Z. J. Gross, P. S. Cunningham, C. Cupit, P. M. Tanguy, A. Guo, X. Moraga, D. Boutet, I. Huvet, A. De Guise, S. Almeida, J. S. Warr, G. W. 2007 STAMPA http://hdl.handle.net/11392/1411407 https://doi.org/10.1007/s10126-007-9041-1 eng eng info:eu-repo/semantics/altIdentifier/wos/WOS:000250207600005 volume:9 firstpage:577 lastpage:591 journal:MARINE BIOTECHNOLOGY http://hdl.handle.net/11392/1411407 doi:10.1007/s10126-007-9041-1 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-35349013661 bivalve Crassostrea giga Crassostrea virginica heterologous hybridization microarray oyster info:eu-repo/semantics/article 2007 ftunivferrarair https://doi.org/10.1007/s10126-007-9041-1 2024-06-19T13:27:50Z The eastern oyster, Crassostrea virginica, and the Pacific oyster, C. gigas, are species of global economic significance as well as important components of estuarine ecosystems and models for genetic and environmental studies. To enhance the molecular tools available for oyster research, an international group of collaborators has constructed a 27,496- feature cDNA microarray containing 4460 sequences derived from C. virginica, 2320 from C. gigas, and 16 non-oyster DNAs serving as positive and negative controls. The performance of the array was assessed by gene expression profiling using gill and digestive gland RNA derived from both C. gigas and C. virginica, and digestive gland RNA from C. ariakensis. The utility of the microarray for detection of homologous genes by cross-hybridization between species was also assessed and the correlation between hybridization intensity and sequence homology for selected genes determined. The oyster cDNA microarray is publicly available to the research community on a cost-recovery basis. Article in Journal/Newspaper Pacific oyster Università degli Studi di Ferrara: CINECA IRIS Pacific Marine Biotechnology 9 5 577 591 |
institution |
Open Polar |
collection |
Università degli Studi di Ferrara: CINECA IRIS |
op_collection_id |
ftunivferrarair |
language |
English |
topic |
bivalve Crassostrea giga Crassostrea virginica heterologous hybridization microarray oyster |
spellingShingle |
bivalve Crassostrea giga Crassostrea virginica heterologous hybridization microarray oyster Jenny M. J. Chapman R. W. Chen Y. A. McKillen D. J. Trent H. F. Lang P. Escoubas J. Bachere E. Buolo V. Liu Z. J. Gross P. S. Cunningham C. Cupit P. M. Tanguy A. Guo X. Moraga D. Boutet I. Huvet A. De Guise S. Almeida J. S. Warr G. W. MANCIA, Annalaura A cDNA Microarray for Crassostrea virginica and C. gigas |
topic_facet |
bivalve Crassostrea giga Crassostrea virginica heterologous hybridization microarray oyster |
description |
The eastern oyster, Crassostrea virginica, and the Pacific oyster, C. gigas, are species of global economic significance as well as important components of estuarine ecosystems and models for genetic and environmental studies. To enhance the molecular tools available for oyster research, an international group of collaborators has constructed a 27,496- feature cDNA microarray containing 4460 sequences derived from C. virginica, 2320 from C. gigas, and 16 non-oyster DNAs serving as positive and negative controls. The performance of the array was assessed by gene expression profiling using gill and digestive gland RNA derived from both C. gigas and C. virginica, and digestive gland RNA from C. ariakensis. The utility of the microarray for detection of homologous genes by cross-hybridization between species was also assessed and the correlation between hybridization intensity and sequence homology for selected genes determined. The oyster cDNA microarray is publicly available to the research community on a cost-recovery basis. |
author2 |
Jenny, M. J. Chapman, R. W. Mancia, Annalaura Chen, Y. A. Mckillen, D. J. Trent, H. F. Lang, P. Escoubas, J. Bachere, E. Buolo, V. Liu, Z. J. Gross, P. S. Cunningham, C. Cupit, P. M. Tanguy, A. Guo, X. Moraga, D. Boutet, I. Huvet, A. De Guise, S. Almeida, J. S. Warr, G. W. |
format |
Article in Journal/Newspaper |
author |
Jenny M. J. Chapman R. W. Chen Y. A. McKillen D. J. Trent H. F. Lang P. Escoubas J. Bachere E. Buolo V. Liu Z. J. Gross P. S. Cunningham C. Cupit P. M. Tanguy A. Guo X. Moraga D. Boutet I. Huvet A. De Guise S. Almeida J. S. Warr G. W. MANCIA, Annalaura |
author_facet |
Jenny M. J. Chapman R. W. Chen Y. A. McKillen D. J. Trent H. F. Lang P. Escoubas J. Bachere E. Buolo V. Liu Z. J. Gross P. S. Cunningham C. Cupit P. M. Tanguy A. Guo X. Moraga D. Boutet I. Huvet A. De Guise S. Almeida J. S. Warr G. W. MANCIA, Annalaura |
author_sort |
Jenny M. J. |
title |
A cDNA Microarray for Crassostrea virginica and C. gigas |
title_short |
A cDNA Microarray for Crassostrea virginica and C. gigas |
title_full |
A cDNA Microarray for Crassostrea virginica and C. gigas |
title_fullStr |
A cDNA Microarray for Crassostrea virginica and C. gigas |
title_full_unstemmed |
A cDNA Microarray for Crassostrea virginica and C. gigas |
title_sort |
cdna microarray for crassostrea virginica and c. gigas |
publishDate |
2007 |
url |
http://hdl.handle.net/11392/1411407 https://doi.org/10.1007/s10126-007-9041-1 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Pacific oyster |
genre_facet |
Pacific oyster |
op_relation |
info:eu-repo/semantics/altIdentifier/wos/WOS:000250207600005 volume:9 firstpage:577 lastpage:591 journal:MARINE BIOTECHNOLOGY http://hdl.handle.net/11392/1411407 doi:10.1007/s10126-007-9041-1 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-35349013661 |
op_doi |
https://doi.org/10.1007/s10126-007-9041-1 |
container_title |
Marine Biotechnology |
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9 |
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5 |
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577 |
op_container_end_page |
591 |
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1809934423142957056 |