Cd2+ and Hg2+ affect glucose release and cAMP-dependent transduction pathway in isolated eel hepatocytes
Isolated hepatocytes of the European eel (Anguilla anguilla) have been used as experimental model to characterize the effects of Cd2+ and Hg2+ on either basal or epinephrine-stimulated glucose release. Cd2+ strongly reduced glucose output from cells perifused in BioGel P4 columns and challenged with...
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ftunivferrarair:oai:sfera.unife.it:11392/1199607 2024-04-14T08:01:00+00:00 Cd2+ and Hg2+ affect glucose release and cAMP-dependent transduction pathway in isolated eel hepatocytes E. FABBRI F. CASELLI A. PIANO G. SARTOR CAPUZZO, Antonio E., Fabbri F., Caselli A., Piano G., Sartor Capuzzo, Antonio 2003 STAMPA http://hdl.handle.net/11392/1199607 https://doi.org/10.1016/S0166-445X(02)00063-2 eng eng info:eu-repo/semantics/altIdentifier/wos/WOS:000180141000006 volume:62 issue:1 firstpage:55 lastpage:65 journal:AQUATIC TOXICOLOGY http://hdl.handle.net/11392/1199607 doi:10.1016/S0166-445X(02)00063-2 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-0037428357 Anguilla anguilla Cadmium cAMP Glucose release Hepatocyte Mercury info:eu-repo/semantics/article 2003 ftunivferrarair https://doi.org/10.1016/S0166-445X(02)00063-2 2024-03-21T17:39:27Z Isolated hepatocytes of the European eel (Anguilla anguilla) have been used as experimental model to characterize the effects of Cd2+ and Hg2+ on either basal or epinephrine-stimulated glucose release. Cd2+ strongly reduced glucose output from cells perifused in BioGel P4 columns and challenged with epinephrine, with a maximum inhibition of 95% reached at 10 μM (IC50 0.04 μM). The epinephrine-stimulated glucose output was also reduced by Hg2+, although a significant inhibition of about 60% was achieved only at 10 μM (IC50 5 μM). The possible influence of Cd2+ and Hg2+ on adenylyl cyclase/cAMP transduction pathway has been investigated, since this system is known to play a pivotal role in the regulation of fish liver glycogen breakdown and consequent glucose release. Micromolar concentrations of both heavy metals significantly reduced the epinephrine-modulated cAMP levels in isolated eel hepatocytes, in good agreement with the reduction of glucose output. Cd2+ and Hg2+ also significantly reduced basal and epinephrine-stimulated adenylyl cyclase activity in liver membrane preparations. A competitive inhibition with respect to Mg2+ was shown by Cd2+ and Hg2+, which significantly reduced the affinity of the allosteric activator for the adenylyl cyclase system. Apparent Km for Mg2+ was 4.35 mM in basal conditions, and increased to 9.1 and 7.1 mM in the presence of 10 μM Cd2+ and Hg2+, respectively. These results indicate that Cd2+ and Hg2+ may impair a crucial intracellular transduction pathway involved in the adrenergic control of glucose metabolism, but also in several other routes of hormonal regulation of liver functions. © 2003 Elsevier Science B.V. All rights reserved. Article in Journal/Newspaper Anguilla anguilla Università degli Studi di Ferrara: CINECA IRIS Aquatic Toxicology 62 1 55 65 |
institution |
Open Polar |
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Università degli Studi di Ferrara: CINECA IRIS |
op_collection_id |
ftunivferrarair |
language |
English |
topic |
Anguilla anguilla Cadmium cAMP Glucose release Hepatocyte Mercury |
spellingShingle |
Anguilla anguilla Cadmium cAMP Glucose release Hepatocyte Mercury E. FABBRI F. CASELLI A. PIANO G. SARTOR CAPUZZO, Antonio Cd2+ and Hg2+ affect glucose release and cAMP-dependent transduction pathway in isolated eel hepatocytes |
topic_facet |
Anguilla anguilla Cadmium cAMP Glucose release Hepatocyte Mercury |
description |
Isolated hepatocytes of the European eel (Anguilla anguilla) have been used as experimental model to characterize the effects of Cd2+ and Hg2+ on either basal or epinephrine-stimulated glucose release. Cd2+ strongly reduced glucose output from cells perifused in BioGel P4 columns and challenged with epinephrine, with a maximum inhibition of 95% reached at 10 μM (IC50 0.04 μM). The epinephrine-stimulated glucose output was also reduced by Hg2+, although a significant inhibition of about 60% was achieved only at 10 μM (IC50 5 μM). The possible influence of Cd2+ and Hg2+ on adenylyl cyclase/cAMP transduction pathway has been investigated, since this system is known to play a pivotal role in the regulation of fish liver glycogen breakdown and consequent glucose release. Micromolar concentrations of both heavy metals significantly reduced the epinephrine-modulated cAMP levels in isolated eel hepatocytes, in good agreement with the reduction of glucose output. Cd2+ and Hg2+ also significantly reduced basal and epinephrine-stimulated adenylyl cyclase activity in liver membrane preparations. A competitive inhibition with respect to Mg2+ was shown by Cd2+ and Hg2+, which significantly reduced the affinity of the allosteric activator for the adenylyl cyclase system. Apparent Km for Mg2+ was 4.35 mM in basal conditions, and increased to 9.1 and 7.1 mM in the presence of 10 μM Cd2+ and Hg2+, respectively. These results indicate that Cd2+ and Hg2+ may impair a crucial intracellular transduction pathway involved in the adrenergic control of glucose metabolism, but also in several other routes of hormonal regulation of liver functions. © 2003 Elsevier Science B.V. All rights reserved. |
author2 |
E., Fabbri F., Caselli A., Piano G., Sartor Capuzzo, Antonio |
format |
Article in Journal/Newspaper |
author |
E. FABBRI F. CASELLI A. PIANO G. SARTOR CAPUZZO, Antonio |
author_facet |
E. FABBRI F. CASELLI A. PIANO G. SARTOR CAPUZZO, Antonio |
author_sort |
E. FABBRI |
title |
Cd2+ and Hg2+ affect glucose release and cAMP-dependent transduction pathway in isolated eel hepatocytes |
title_short |
Cd2+ and Hg2+ affect glucose release and cAMP-dependent transduction pathway in isolated eel hepatocytes |
title_full |
Cd2+ and Hg2+ affect glucose release and cAMP-dependent transduction pathway in isolated eel hepatocytes |
title_fullStr |
Cd2+ and Hg2+ affect glucose release and cAMP-dependent transduction pathway in isolated eel hepatocytes |
title_full_unstemmed |
Cd2+ and Hg2+ affect glucose release and cAMP-dependent transduction pathway in isolated eel hepatocytes |
title_sort |
cd2+ and hg2+ affect glucose release and camp-dependent transduction pathway in isolated eel hepatocytes |
publishDate |
2003 |
url |
http://hdl.handle.net/11392/1199607 https://doi.org/10.1016/S0166-445X(02)00063-2 |
genre |
Anguilla anguilla |
genre_facet |
Anguilla anguilla |
op_relation |
info:eu-repo/semantics/altIdentifier/wos/WOS:000180141000006 volume:62 issue:1 firstpage:55 lastpage:65 journal:AQUATIC TOXICOLOGY http://hdl.handle.net/11392/1199607 doi:10.1016/S0166-445X(02)00063-2 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-0037428357 |
op_doi |
https://doi.org/10.1016/S0166-445X(02)00063-2 |
container_title |
Aquatic Toxicology |
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62 |
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1 |
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55 |
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65 |
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1796306200266014720 |