G proteins immunodetection and adrenergic transduction pathways in the liver of Anguilla anguilla
G proteins are members of a highly conserved superfamily of GTPases, which includes heterotrimeric (alpha, beta, gamma) proteins acting as critical control points for transmembrane signaling. In ectothermal vertebrates, knowledge about these proteins is scarce, and our work provides the first demons...
Published in: | Physiological and Biochemical Zoology |
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Online Access: | http://hdl.handle.net/11392/1199606 https://doi.org/10.1086/345483 |
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ftunivferrarair:oai:sfera.unife.it:11392/1199606 2024-04-14T08:00:59+00:00 G proteins immunodetection and adrenergic transduction pathways in the liver of Anguilla anguilla F. CASELLI A. PIANO P. VALBONESI E. FABBRI CAPUZZO, Antonio F., Caselli Capuzzo, Antonio A., Piano P., Valbonesi E., Fabbri 2002 STAMPA http://hdl.handle.net/11392/1199606 https://doi.org/10.1086/345483 eng eng info:eu-repo/semantics/altIdentifier/wos/WOS:000181460100009 volume:75 issue:6 firstpage:609 lastpage:616 journal:PHYSIOLOGICAL AND BIOCHEMICAL ZOOLOGY http://hdl.handle.net/11392/1199606 doi:10.1086/345483 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-0242669241 info:eu-repo/semantics/article 2002 ftunivferrarair https://doi.org/10.1086/345483 2024-03-21T17:39:27Z G proteins are members of a highly conserved superfamily of GTPases, which includes heterotrimeric (alpha, beta, gamma) proteins acting as critical control points for transmembrane signaling. In ectothermal vertebrates, knowledge about these proteins is scarce, and our work provides the first demonstration that G(s), G(q), and G(i) proteins are all present in the liver of a fish. G(q)alpha subunits of about 42 kDa have been identified in European eel (Anguilla anguilla) liver membranes, supporting previous reports about the existence of hormone transduction pathways coupled to inositol 1,4,5-trisphosphate/Ca(2+) enhancement in fish hepatocytes. Although two G(s)alpha proteins of about 45 and 52 kDa have been reported in mammals, a single isoform of approximately 45 kDa has been recognized in eel liver. G(s)alpha and G(q)alpha proteins are involved in the epinephrine transduction pathway, leading to cAMP and Ca(2+) intracellular increments, respectively. Interestingly, both messengers significantly stimulated glucose release from eel hepatocytes but with a different time course. In fact, the Ca(2+)-dependent glucose output preceded the cAMP-mediated release by about 7 min. G(i)alpha subunits of about 40 kDa were also immunodetected, suggesting the presence of hormone receptors leading to adenylyl cyclase inhibition in eel liver; however, alpha(2)- adrenoreceptor ligands were ineffective on both enzyme activity and glucose release. Article in Journal/Newspaper Anguilla anguilla Università degli Studi di Ferrara: CINECA IRIS Physiological and Biochemical Zoology 75 6 609 616 |
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Open Polar |
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Università degli Studi di Ferrara: CINECA IRIS |
op_collection_id |
ftunivferrarair |
language |
English |
description |
G proteins are members of a highly conserved superfamily of GTPases, which includes heterotrimeric (alpha, beta, gamma) proteins acting as critical control points for transmembrane signaling. In ectothermal vertebrates, knowledge about these proteins is scarce, and our work provides the first demonstration that G(s), G(q), and G(i) proteins are all present in the liver of a fish. G(q)alpha subunits of about 42 kDa have been identified in European eel (Anguilla anguilla) liver membranes, supporting previous reports about the existence of hormone transduction pathways coupled to inositol 1,4,5-trisphosphate/Ca(2+) enhancement in fish hepatocytes. Although two G(s)alpha proteins of about 45 and 52 kDa have been reported in mammals, a single isoform of approximately 45 kDa has been recognized in eel liver. G(s)alpha and G(q)alpha proteins are involved in the epinephrine transduction pathway, leading to cAMP and Ca(2+) intracellular increments, respectively. Interestingly, both messengers significantly stimulated glucose release from eel hepatocytes but with a different time course. In fact, the Ca(2+)-dependent glucose output preceded the cAMP-mediated release by about 7 min. G(i)alpha subunits of about 40 kDa were also immunodetected, suggesting the presence of hormone receptors leading to adenylyl cyclase inhibition in eel liver; however, alpha(2)- adrenoreceptor ligands were ineffective on both enzyme activity and glucose release. |
author2 |
F., Caselli Capuzzo, Antonio A., Piano P., Valbonesi E., Fabbri |
format |
Article in Journal/Newspaper |
author |
F. CASELLI A. PIANO P. VALBONESI E. FABBRI CAPUZZO, Antonio |
spellingShingle |
F. CASELLI A. PIANO P. VALBONESI E. FABBRI CAPUZZO, Antonio G proteins immunodetection and adrenergic transduction pathways in the liver of Anguilla anguilla |
author_facet |
F. CASELLI A. PIANO P. VALBONESI E. FABBRI CAPUZZO, Antonio |
author_sort |
F. CASELLI |
title |
G proteins immunodetection and adrenergic transduction pathways in the liver of Anguilla anguilla |
title_short |
G proteins immunodetection and adrenergic transduction pathways in the liver of Anguilla anguilla |
title_full |
G proteins immunodetection and adrenergic transduction pathways in the liver of Anguilla anguilla |
title_fullStr |
G proteins immunodetection and adrenergic transduction pathways in the liver of Anguilla anguilla |
title_full_unstemmed |
G proteins immunodetection and adrenergic transduction pathways in the liver of Anguilla anguilla |
title_sort |
g proteins immunodetection and adrenergic transduction pathways in the liver of anguilla anguilla |
publishDate |
2002 |
url |
http://hdl.handle.net/11392/1199606 https://doi.org/10.1086/345483 |
genre |
Anguilla anguilla |
genre_facet |
Anguilla anguilla |
op_relation |
info:eu-repo/semantics/altIdentifier/wos/WOS:000181460100009 volume:75 issue:6 firstpage:609 lastpage:616 journal:PHYSIOLOGICAL AND BIOCHEMICAL ZOOLOGY http://hdl.handle.net/11392/1199606 doi:10.1086/345483 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-0242669241 |
op_doi |
https://doi.org/10.1086/345483 |
container_title |
Physiological and Biochemical Zoology |
container_volume |
75 |
container_issue |
6 |
container_start_page |
609 |
op_container_end_page |
616 |
_version_ |
1796305930661396480 |