Immunolocalization of steroidogenic enzymes in gonads of European eel, Anguilla anguilla (L.)
The localization of cytochrome P450 cholesterol side-chain cleavage (P450scc), 3β-hydroxysteroid dehydrogenase (3β-HSD) and aromatase (P450arom) was investigated using polyclonal antibodies during gonad development in wild European eels, Anguilla anguilla (L.), from River Po Delta (Ferrara, Italy)....
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Online Access: | http://hdl.handle.net/11392/535704 https://doi.org/10.1111/j.1439-0426.2009.01350.x |
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ftunivferrarair:oai:iris.unife.it:11392/535704 2024-02-11T09:55:29+01:00 Immunolocalization of steroidogenic enzymes in gonads of European eel, Anguilla anguilla (L.) GRANDI, Gilberto CHICCA, Milvia ROSSI, Remigio E. Tagliati M. Arveda Grandi, Gilberto Chicca, Milvia E., Tagliati M., Arveda Rossi, Remigio 2010 STAMPA http://hdl.handle.net/11392/535704 https://doi.org/10.1111/j.1439-0426.2009.01350.x eng eng info:eu-repo/semantics/altIdentifier/wos/WOS:000273821900008 volume:26 issue:1 firstpage:40 lastpage:48 journal:JOURNAL OF APPLIED ICHTHYOLOGY http://hdl.handle.net/11392/535704 doi:10.1111/j.1439-0426.2009.01350.x info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-75649117373 Immunohistochemistry steroidogenic cell head kidney cytochrome P450 cholesterol side-chain cleavage - 3β-hydroxysteroid dehydrogenase - P450 aromatase info:eu-repo/semantics/article 2010 ftunivferrarair https://doi.org/10.1111/j.1439-0426.2009.01350.x 2024-01-24T17:34:06Z The localization of cytochrome P450 cholesterol side-chain cleavage (P450scc), 3β-hydroxysteroid dehydrogenase (3β-HSD) and aromatase (P450arom) was investigated using polyclonal antibodies during gonad development in wild European eels, Anguilla anguilla (L.), from River Po Delta (Ferrara, Italy). The first steroidogenic cells, observed in undifferentiated gonads of 14-16 cm yellow eels, showed no P450scc, 3β-HSD and P450arom activity, but positive regions appeared in head kidney insulae from this stage until the silver eel one. In undifferentiated gonads of 16-20 cm yellow eels the steroidogenic cells were positive to all enzymes. Pre-Leydig steroidogenic cells, identified in Syrski organs of yellow eels of 22-26 cm evolving into testes, were positive to 3β-HSD and P450scc, but negative to P450arom; however, steroidogenic cells in Syrski organs evolving towards ovaries and in small but fully differentiated ovaries were positive to all enzymes. Immature testes of yellow and silver eels had Leydig cells positive to P450scc and 3β-HSD: the same reactions were also observed in some Sertoli cells of silver eel testes containing meiotic cells. Sex differentiation in A. anguilla apparently occurs through an initial female stage controlled by P450arom activity. Leydig and Sertoli cells appear involved in different steps of hormonal control of spermatogenesis: Leydig cells begin their steroidogenic activity before meiosis, while Sertoli cells begin their activity during meiosis. Article in Journal/Newspaper Anguilla anguilla Università degli Studi di Ferrara: CINECA IRIS Ferrara ENVELOPE(-41.391,-41.391,-82.255,-82.255) Journal of Applied Ichthyology 26 1 40 48 |
institution |
Open Polar |
collection |
Università degli Studi di Ferrara: CINECA IRIS |
op_collection_id |
ftunivferrarair |
language |
English |
topic |
Immunohistochemistry steroidogenic cell head kidney cytochrome P450 cholesterol side-chain cleavage - 3β-hydroxysteroid dehydrogenase - P450 aromatase |
spellingShingle |
Immunohistochemistry steroidogenic cell head kidney cytochrome P450 cholesterol side-chain cleavage - 3β-hydroxysteroid dehydrogenase - P450 aromatase GRANDI, Gilberto CHICCA, Milvia ROSSI, Remigio E. Tagliati M. Arveda Immunolocalization of steroidogenic enzymes in gonads of European eel, Anguilla anguilla (L.) |
topic_facet |
Immunohistochemistry steroidogenic cell head kidney cytochrome P450 cholesterol side-chain cleavage - 3β-hydroxysteroid dehydrogenase - P450 aromatase |
description |
The localization of cytochrome P450 cholesterol side-chain cleavage (P450scc), 3β-hydroxysteroid dehydrogenase (3β-HSD) and aromatase (P450arom) was investigated using polyclonal antibodies during gonad development in wild European eels, Anguilla anguilla (L.), from River Po Delta (Ferrara, Italy). The first steroidogenic cells, observed in undifferentiated gonads of 14-16 cm yellow eels, showed no P450scc, 3β-HSD and P450arom activity, but positive regions appeared in head kidney insulae from this stage until the silver eel one. In undifferentiated gonads of 16-20 cm yellow eels the steroidogenic cells were positive to all enzymes. Pre-Leydig steroidogenic cells, identified in Syrski organs of yellow eels of 22-26 cm evolving into testes, were positive to 3β-HSD and P450scc, but negative to P450arom; however, steroidogenic cells in Syrski organs evolving towards ovaries and in small but fully differentiated ovaries were positive to all enzymes. Immature testes of yellow and silver eels had Leydig cells positive to P450scc and 3β-HSD: the same reactions were also observed in some Sertoli cells of silver eel testes containing meiotic cells. Sex differentiation in A. anguilla apparently occurs through an initial female stage controlled by P450arom activity. Leydig and Sertoli cells appear involved in different steps of hormonal control of spermatogenesis: Leydig cells begin their steroidogenic activity before meiosis, while Sertoli cells begin their activity during meiosis. |
author2 |
Grandi, Gilberto Chicca, Milvia E., Tagliati M., Arveda Rossi, Remigio |
format |
Article in Journal/Newspaper |
author |
GRANDI, Gilberto CHICCA, Milvia ROSSI, Remigio E. Tagliati M. Arveda |
author_facet |
GRANDI, Gilberto CHICCA, Milvia ROSSI, Remigio E. Tagliati M. Arveda |
author_sort |
GRANDI, Gilberto |
title |
Immunolocalization of steroidogenic enzymes in gonads of European eel, Anguilla anguilla (L.) |
title_short |
Immunolocalization of steroidogenic enzymes in gonads of European eel, Anguilla anguilla (L.) |
title_full |
Immunolocalization of steroidogenic enzymes in gonads of European eel, Anguilla anguilla (L.) |
title_fullStr |
Immunolocalization of steroidogenic enzymes in gonads of European eel, Anguilla anguilla (L.) |
title_full_unstemmed |
Immunolocalization of steroidogenic enzymes in gonads of European eel, Anguilla anguilla (L.) |
title_sort |
immunolocalization of steroidogenic enzymes in gonads of european eel, anguilla anguilla (l.) |
publishDate |
2010 |
url |
http://hdl.handle.net/11392/535704 https://doi.org/10.1111/j.1439-0426.2009.01350.x |
long_lat |
ENVELOPE(-41.391,-41.391,-82.255,-82.255) |
geographic |
Ferrara |
geographic_facet |
Ferrara |
genre |
Anguilla anguilla |
genre_facet |
Anguilla anguilla |
op_relation |
info:eu-repo/semantics/altIdentifier/wos/WOS:000273821900008 volume:26 issue:1 firstpage:40 lastpage:48 journal:JOURNAL OF APPLIED ICHTHYOLOGY http://hdl.handle.net/11392/535704 doi:10.1111/j.1439-0426.2009.01350.x info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-75649117373 |
op_doi |
https://doi.org/10.1111/j.1439-0426.2009.01350.x |
container_title |
Journal of Applied Ichthyology |
container_volume |
26 |
container_issue |
1 |
container_start_page |
40 |
op_container_end_page |
48 |
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1790596590661009408 |