An integrated approach to the conservation and management of the European eel in the mediterranean region: immunolocalization of steroidogenic enzymes during sex differentiation
Studies on steroidogenic cells producing key enzymes during sex differentiation are relevant for eel conservation and sustainable stock management. The enzymes cytochrome P450 side-chain cleavage (P450scc), 3-beta-hydroxysteroid dehydrogenase (3β-HSD) and aromatase (CYP450) play a key role in steroi...
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European Aquaculture Society and hosted by Polskie Towarzytwo Rybackie (the Polish Fisheries Association) 2008
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Online Access: | http://hdl.handle.net/11392/531205 |
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ftunivferrarair:oai:iris.unife.it:11392/531205 2024-01-14T09:59:16+01:00 An integrated approach to the conservation and management of the European eel in the mediterranean region: immunolocalization of steroidogenic enzymes during sex differentiation GRANDI, Gilberto CHICCA, Milvia ROSSI, Remigio Tagliati E. De Leo G. E. Kamler, K. Dabrowski Grandi, Gilberto Chicca, Milvia Tagliati, E. Rossi, Remigio De Leo, G. 2008 STAMPA http://hdl.handle.net/11392/531205 eng eng European Aquaculture Society and hosted by Polskie Towarzytwo Rybackie (the Polish Fisheries Association) 2008 country:POL place:Olsztyn info:eu-repo/semantics/altIdentifier/isbn/9788360111307 ispartofbook:Resource management - natural, human and material for the sustainable development of aquaculture Aquaculture Europe, 2008 volume:37 firstpage:248 lastpage:249 http://hdl.handle.net/11392/531205 Anguilla anguilla Gonad Sex Differentiation Steroidogenic enzyme Immunolocalization info:eu-repo/semantics/conferenceObject 2008 ftunivferrarair 2023-12-20T17:34:19Z Studies on steroidogenic cells producing key enzymes during sex differentiation are relevant for eel conservation and sustainable stock management. The enzymes cytochrome P450 side-chain cleavage (P450scc), 3-beta-hydroxysteroid dehydrogenase (3β-HSD) and aromatase (CYP450) play a key role in steroid metabolism. The immunolocalization of CYP450scc, 3β-HSD and aromatase, previously studied in some teleosts (Kobayashi et al., 1998; Bhandari et al., 2006), was investigated in wild European eels (Anguilla anguilla) from Valli di Comacchio, Ferrara (Italy). Sections of the entire gonad were exposed to goat polyclonal antibodies and prepared for optical microscopy (Grandi et al., 2003). The first steroidogenic cells, observed in undifferentiated gonads of 14-16 cm eels, did not show P450scc, 3β-HSD and aromatase activity, but a positive immunolocalization appeared at the same stage in cephalic kidney (interrenal) regions. Steroidogenic cells positive to all enzymes appeared in undifferentiated gonads of 16-20 cm yellow eels. Pre-Leydig steroidogenic cells, identified in Syrski organs of 22-28 cm yellow eels, were positive to 3β-HSD and P450scc, but negative to aromatase; however, steroidogenic cells identified in ovarian interstitial tissue were positive to all enzymes. In initial ovaries of yellow eels of the same length, some cells, identified as presumptive thecal cells, resulted positive to all three enzymes.Thus sex differentiation apparently shows an initial female stage, probably through control of aromatase activity. The immature testes of yellow and silver eels have large clusters of Leydig cells showing immunostaining with P450scc and 3β-HSD antisera Conference Object Anguilla anguilla Università degli Studi di Ferrara: CINECA IRIS Grandi ENVELOPE(-23.471,-23.471,65.845,65.845) Ferrara ENVELOPE(-41.391,-41.391,-82.255,-82.255) |
institution |
Open Polar |
collection |
Università degli Studi di Ferrara: CINECA IRIS |
op_collection_id |
ftunivferrarair |
language |
English |
topic |
Anguilla anguilla Gonad Sex Differentiation Steroidogenic enzyme Immunolocalization |
spellingShingle |
Anguilla anguilla Gonad Sex Differentiation Steroidogenic enzyme Immunolocalization GRANDI, Gilberto CHICCA, Milvia ROSSI, Remigio Tagliati E. De Leo G. An integrated approach to the conservation and management of the European eel in the mediterranean region: immunolocalization of steroidogenic enzymes during sex differentiation |
topic_facet |
Anguilla anguilla Gonad Sex Differentiation Steroidogenic enzyme Immunolocalization |
description |
Studies on steroidogenic cells producing key enzymes during sex differentiation are relevant for eel conservation and sustainable stock management. The enzymes cytochrome P450 side-chain cleavage (P450scc), 3-beta-hydroxysteroid dehydrogenase (3β-HSD) and aromatase (CYP450) play a key role in steroid metabolism. The immunolocalization of CYP450scc, 3β-HSD and aromatase, previously studied in some teleosts (Kobayashi et al., 1998; Bhandari et al., 2006), was investigated in wild European eels (Anguilla anguilla) from Valli di Comacchio, Ferrara (Italy). Sections of the entire gonad were exposed to goat polyclonal antibodies and prepared for optical microscopy (Grandi et al., 2003). The first steroidogenic cells, observed in undifferentiated gonads of 14-16 cm eels, did not show P450scc, 3β-HSD and aromatase activity, but a positive immunolocalization appeared at the same stage in cephalic kidney (interrenal) regions. Steroidogenic cells positive to all enzymes appeared in undifferentiated gonads of 16-20 cm yellow eels. Pre-Leydig steroidogenic cells, identified in Syrski organs of 22-28 cm yellow eels, were positive to 3β-HSD and P450scc, but negative to aromatase; however, steroidogenic cells identified in ovarian interstitial tissue were positive to all enzymes. In initial ovaries of yellow eels of the same length, some cells, identified as presumptive thecal cells, resulted positive to all three enzymes.Thus sex differentiation apparently shows an initial female stage, probably through control of aromatase activity. The immature testes of yellow and silver eels have large clusters of Leydig cells showing immunostaining with P450scc and 3β-HSD antisera |
author2 |
E. Kamler, K. Dabrowski Grandi, Gilberto Chicca, Milvia Tagliati, E. Rossi, Remigio De Leo, G. |
format |
Conference Object |
author |
GRANDI, Gilberto CHICCA, Milvia ROSSI, Remigio Tagliati E. De Leo G. |
author_facet |
GRANDI, Gilberto CHICCA, Milvia ROSSI, Remigio Tagliati E. De Leo G. |
author_sort |
GRANDI, Gilberto |
title |
An integrated approach to the conservation and management of the European eel in the mediterranean region: immunolocalization of steroidogenic enzymes during sex differentiation |
title_short |
An integrated approach to the conservation and management of the European eel in the mediterranean region: immunolocalization of steroidogenic enzymes during sex differentiation |
title_full |
An integrated approach to the conservation and management of the European eel in the mediterranean region: immunolocalization of steroidogenic enzymes during sex differentiation |
title_fullStr |
An integrated approach to the conservation and management of the European eel in the mediterranean region: immunolocalization of steroidogenic enzymes during sex differentiation |
title_full_unstemmed |
An integrated approach to the conservation and management of the European eel in the mediterranean region: immunolocalization of steroidogenic enzymes during sex differentiation |
title_sort |
integrated approach to the conservation and management of the european eel in the mediterranean region: immunolocalization of steroidogenic enzymes during sex differentiation |
publisher |
European Aquaculture Society and hosted by Polskie Towarzytwo Rybackie (the Polish Fisheries Association) 2008 |
publishDate |
2008 |
url |
http://hdl.handle.net/11392/531205 |
long_lat |
ENVELOPE(-23.471,-23.471,65.845,65.845) ENVELOPE(-41.391,-41.391,-82.255,-82.255) |
geographic |
Grandi Ferrara |
geographic_facet |
Grandi Ferrara |
genre |
Anguilla anguilla |
genre_facet |
Anguilla anguilla |
op_relation |
info:eu-repo/semantics/altIdentifier/isbn/9788360111307 ispartofbook:Resource management - natural, human and material for the sustainable development of aquaculture Aquaculture Europe, 2008 volume:37 firstpage:248 lastpage:249 http://hdl.handle.net/11392/531205 |
_version_ |
1788057886476206080 |