Anguilla anguilla intestinal immune response to natural infection with Contracaecum rudolphii A larvae
The European eel, Anguilla anguilla, is a major warm-water fish species cultured in North and South Europe. Seventy-one A. anguilla collected between 2010 and 2015 from the Comacchio lagoons were examined. Fish were infected and damaged by larvae (L3) of the nematode Contracaecum rudolphii A, which...
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Online Access: | http://hdl.handle.net/11392/2343628 https://doi.org/10.1111/jfd.12455 https://onlinelibrary.wiley.com/doi/full/10.1111/jfd.12455 |
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ftunivferrarair:oai:iris.unife.it:11392/2343628 2024-01-28T09:58:34+01:00 Anguilla anguilla intestinal immune response to natural infection with Contracaecum rudolphii A larvae SAYYAF DEZFULI, Bahram Manera, M Bosi, G Depasquale, J. A D'Amelio, S CASTALDELLI, Giuseppe GIARI, Luisa SAYYAF DEZFULI, Bahram Manera, M Bosi, G Depasquale, J. A D'Amelio, S Castaldelli, Giuseppe Giari, Luisa 2016 STAMPA http://hdl.handle.net/11392/2343628 https://doi.org/10.1111/jfd.12455 https://onlinelibrary.wiley.com/doi/full/10.1111/jfd.12455 eng eng info:eu-repo/semantics/altIdentifier/pmid/26814373 info:eu-repo/semantics/altIdentifier/wos/WOS:000387108800005 volume:39 issue:10 firstpage:1187 lastpage:1200 numberofpages:14 journal:JOURNAL OF FISH DISEASES http://hdl.handle.net/11392/2343628 doi:10.1111/jfd.12455 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85027957796 https://onlinelibrary.wiley.com/doi/full/10.1111/jfd.12455 info:eu-repo/semantics/openAccess European eel fish nematodes granuloma immune cells PCNA info:eu-repo/semantics/article 2016 ftunivferrarair https://doi.org/10.1111/jfd.12455 2024-01-17T17:37:34Z The European eel, Anguilla anguilla, is a major warm-water fish species cultured in North and South Europe. Seventy-one A. anguilla collected between 2010 and 2015 from the Comacchio lagoons were examined. Fish were infected and damaged by larvae (L3) of the nematode Contracaecum rudolphii A, which were encapsulated within the thickness of the intestinal wall and within the external visceral peritoneum (serosa). Conspicuous granulomas, visible at sites of infection, were arranged in a trilayer, formed by a series of concentric whorls. The cells involved in the immune response and their distribution in the granuloma layers were assessed by immunohistochemical, immunofluorescence, and ultrastructural techniques. The outer part of the granuloma contained macrophages, macrophage aggregates, and mast cells (MCs) scattered among fibroblasts. This layer was vascularized, with degranulation of MCs occurring in close proximity to the capillaries. The middle layer was rich in MCs and fibroblasts. The inner layer, closest to the parasite larva, consisted mainly of dark epithelioid cells, some of which were necrotic. Non-necrotic epithelioid cells formed desmosomes between themselves or with fibroblasts. Within the granulomas, numerous cells of different types were positive to proliferative cell nuclear antigen antibody, indicating a high degree of cellular proliferation around the larvae. Article in Journal/Newspaper Anguilla anguilla European eel Università degli Studi di Ferrara: CINECA IRIS Journal of Fish Diseases 39 10 1187 1200 |
institution |
Open Polar |
collection |
Università degli Studi di Ferrara: CINECA IRIS |
op_collection_id |
ftunivferrarair |
language |
English |
topic |
European eel fish nematodes granuloma immune cells PCNA |
spellingShingle |
European eel fish nematodes granuloma immune cells PCNA SAYYAF DEZFULI, Bahram Manera, M Bosi, G Depasquale, J. A D'Amelio, S CASTALDELLI, Giuseppe GIARI, Luisa Anguilla anguilla intestinal immune response to natural infection with Contracaecum rudolphii A larvae |
topic_facet |
European eel fish nematodes granuloma immune cells PCNA |
description |
The European eel, Anguilla anguilla, is a major warm-water fish species cultured in North and South Europe. Seventy-one A. anguilla collected between 2010 and 2015 from the Comacchio lagoons were examined. Fish were infected and damaged by larvae (L3) of the nematode Contracaecum rudolphii A, which were encapsulated within the thickness of the intestinal wall and within the external visceral peritoneum (serosa). Conspicuous granulomas, visible at sites of infection, were arranged in a trilayer, formed by a series of concentric whorls. The cells involved in the immune response and their distribution in the granuloma layers were assessed by immunohistochemical, immunofluorescence, and ultrastructural techniques. The outer part of the granuloma contained macrophages, macrophage aggregates, and mast cells (MCs) scattered among fibroblasts. This layer was vascularized, with degranulation of MCs occurring in close proximity to the capillaries. The middle layer was rich in MCs and fibroblasts. The inner layer, closest to the parasite larva, consisted mainly of dark epithelioid cells, some of which were necrotic. Non-necrotic epithelioid cells formed desmosomes between themselves or with fibroblasts. Within the granulomas, numerous cells of different types were positive to proliferative cell nuclear antigen antibody, indicating a high degree of cellular proliferation around the larvae. |
author2 |
SAYYAF DEZFULI, Bahram Manera, M Bosi, G Depasquale, J. A D'Amelio, S Castaldelli, Giuseppe Giari, Luisa |
format |
Article in Journal/Newspaper |
author |
SAYYAF DEZFULI, Bahram Manera, M Bosi, G Depasquale, J. A D'Amelio, S CASTALDELLI, Giuseppe GIARI, Luisa |
author_facet |
SAYYAF DEZFULI, Bahram Manera, M Bosi, G Depasquale, J. A D'Amelio, S CASTALDELLI, Giuseppe GIARI, Luisa |
author_sort |
SAYYAF DEZFULI, Bahram |
title |
Anguilla anguilla intestinal immune response to natural infection with Contracaecum rudolphii A larvae |
title_short |
Anguilla anguilla intestinal immune response to natural infection with Contracaecum rudolphii A larvae |
title_full |
Anguilla anguilla intestinal immune response to natural infection with Contracaecum rudolphii A larvae |
title_fullStr |
Anguilla anguilla intestinal immune response to natural infection with Contracaecum rudolphii A larvae |
title_full_unstemmed |
Anguilla anguilla intestinal immune response to natural infection with Contracaecum rudolphii A larvae |
title_sort |
anguilla anguilla intestinal immune response to natural infection with contracaecum rudolphii a larvae |
publishDate |
2016 |
url |
http://hdl.handle.net/11392/2343628 https://doi.org/10.1111/jfd.12455 https://onlinelibrary.wiley.com/doi/full/10.1111/jfd.12455 |
genre |
Anguilla anguilla European eel |
genre_facet |
Anguilla anguilla European eel |
op_relation |
info:eu-repo/semantics/altIdentifier/pmid/26814373 info:eu-repo/semantics/altIdentifier/wos/WOS:000387108800005 volume:39 issue:10 firstpage:1187 lastpage:1200 numberofpages:14 journal:JOURNAL OF FISH DISEASES http://hdl.handle.net/11392/2343628 doi:10.1111/jfd.12455 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85027957796 https://onlinelibrary.wiley.com/doi/full/10.1111/jfd.12455 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1111/jfd.12455 |
container_title |
Journal of Fish Diseases |
container_volume |
39 |
container_issue |
10 |
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1187 |
op_container_end_page |
1200 |
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1789328235618631680 |