Cutaneous myxidiosis in European eel, Anguilla anguilla (Linnaeus, 1758): Histopathology, histochemistry and laminin immunohistochemistry
Histopathology, histochemistry and immunohistochemistry of the integument of European eel, Anguilla anguilla (Linnaeus, 1758), infected by Myxidium sp. are reported. Skin samples from affected and unaffected eels were dissected, formalin fixed, paraffin embedded, sectioned and stained with H&E,...
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ftunivferrarair:oai:iris.unife.it:11392/2337781 2023-05-15T13:27:07+02:00 Cutaneous myxidiosis in European eel, Anguilla anguilla (Linnaeus, 1758): Histopathology, histochemistry and laminin immunohistochemistry Manera, M Borreca, C. SAYYAF DEZFULI, Bahram Manera, M Borreca, C. SAYYAF DEZFULI, Bahram 2016 STAMPA http://hdl.handle.net/11392/2337781 https://doi.org/10.1111/jfd.12418 http://www.blackwellpublishing.com/jfd eng eng volume:39 firstpage:845 lastpage:851 numberofpages:7 journal:JOURNAL OF FISH DISEASES http://hdl.handle.net/11392/2337781 doi:10.1111/jfd.12418 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85027929694 http://www.blackwellpublishing.com/jfd Histochemistry Histopathology Immune evasion Immunohistochemistry Laminin Myxidium spp info:eu-repo/semantics/article 2016 ftunivferrarair https://doi.org/10.1111/jfd.12418 2023-03-01T21:17:55Z Histopathology, histochemistry and immunohistochemistry of the integument of European eel, Anguilla anguilla (Linnaeus, 1758), infected by Myxidium sp. are reported. Skin samples from affected and unaffected eels were dissected, formalin fixed, paraffin embedded, sectioned and stained with H&E, Periodic acid–Schiff's staining method, Alcian Blue 8 GX pH 2.5/Periodic acid–Schiff's and McCallum-Goodpasture's Gram stain. Moreover, immunohistochemistry was performed using a primary polyclonal laminin antibody. Histologically, cysts (diameter 2–3 mm) were observed mainly under the scale pockets, encircled by a thin collagen layer, lined by elongated, flattened fibroblasts and containing bipolar, PAS- and Gram-positive spores with opposite polar capsules. The epidermis stretched by the underlying cyst appeared dysplastic, thinned with a significant reduction in mucous cells number. Only inconsistent and aspecific inflammatory reaction was noted around the cysts at the dermis/epidermis interface. Intense laminin-like protein immunolabel was documented in the plasmodial ectoplasm and related to host anergia. This was the first report of laminin immunolabel in a member of the Myxozoa. Epidermal dysplasia represents likely an aspecific response against the underlying tensile force exerted by the developing parasite cyst, while fibroblast and collagen encapsulation denote a parasite-driven host response protecting, rather than harming, the encircled parasite. Article in Journal/Newspaper Anguilla anguilla Università degli Studi di Ferrara: CINECA IRIS McCallum ENVELOPE(78.017,78.017,-68.628,-68.628) Journal of Fish Diseases 39 7 845 851 |
institution |
Open Polar |
collection |
Università degli Studi di Ferrara: CINECA IRIS |
op_collection_id |
ftunivferrarair |
language |
English |
topic |
Histochemistry Histopathology Immune evasion Immunohistochemistry Laminin Myxidium spp |
spellingShingle |
Histochemistry Histopathology Immune evasion Immunohistochemistry Laminin Myxidium spp Manera, M Borreca, C. SAYYAF DEZFULI, Bahram Cutaneous myxidiosis in European eel, Anguilla anguilla (Linnaeus, 1758): Histopathology, histochemistry and laminin immunohistochemistry |
topic_facet |
Histochemistry Histopathology Immune evasion Immunohistochemistry Laminin Myxidium spp |
description |
Histopathology, histochemistry and immunohistochemistry of the integument of European eel, Anguilla anguilla (Linnaeus, 1758), infected by Myxidium sp. are reported. Skin samples from affected and unaffected eels were dissected, formalin fixed, paraffin embedded, sectioned and stained with H&E, Periodic acid–Schiff's staining method, Alcian Blue 8 GX pH 2.5/Periodic acid–Schiff's and McCallum-Goodpasture's Gram stain. Moreover, immunohistochemistry was performed using a primary polyclonal laminin antibody. Histologically, cysts (diameter 2–3 mm) were observed mainly under the scale pockets, encircled by a thin collagen layer, lined by elongated, flattened fibroblasts and containing bipolar, PAS- and Gram-positive spores with opposite polar capsules. The epidermis stretched by the underlying cyst appeared dysplastic, thinned with a significant reduction in mucous cells number. Only inconsistent and aspecific inflammatory reaction was noted around the cysts at the dermis/epidermis interface. Intense laminin-like protein immunolabel was documented in the plasmodial ectoplasm and related to host anergia. This was the first report of laminin immunolabel in a member of the Myxozoa. Epidermal dysplasia represents likely an aspecific response against the underlying tensile force exerted by the developing parasite cyst, while fibroblast and collagen encapsulation denote a parasite-driven host response protecting, rather than harming, the encircled parasite. |
author2 |
Manera, M Borreca, C. SAYYAF DEZFULI, Bahram |
format |
Article in Journal/Newspaper |
author |
Manera, M Borreca, C. SAYYAF DEZFULI, Bahram |
author_facet |
Manera, M Borreca, C. SAYYAF DEZFULI, Bahram |
author_sort |
Manera, M |
title |
Cutaneous myxidiosis in European eel, Anguilla anguilla (Linnaeus, 1758): Histopathology, histochemistry and laminin immunohistochemistry |
title_short |
Cutaneous myxidiosis in European eel, Anguilla anguilla (Linnaeus, 1758): Histopathology, histochemistry and laminin immunohistochemistry |
title_full |
Cutaneous myxidiosis in European eel, Anguilla anguilla (Linnaeus, 1758): Histopathology, histochemistry and laminin immunohistochemistry |
title_fullStr |
Cutaneous myxidiosis in European eel, Anguilla anguilla (Linnaeus, 1758): Histopathology, histochemistry and laminin immunohistochemistry |
title_full_unstemmed |
Cutaneous myxidiosis in European eel, Anguilla anguilla (Linnaeus, 1758): Histopathology, histochemistry and laminin immunohistochemistry |
title_sort |
cutaneous myxidiosis in european eel, anguilla anguilla (linnaeus, 1758): histopathology, histochemistry and laminin immunohistochemistry |
publishDate |
2016 |
url |
http://hdl.handle.net/11392/2337781 https://doi.org/10.1111/jfd.12418 http://www.blackwellpublishing.com/jfd |
long_lat |
ENVELOPE(78.017,78.017,-68.628,-68.628) |
geographic |
McCallum |
geographic_facet |
McCallum |
genre |
Anguilla anguilla |
genre_facet |
Anguilla anguilla |
op_relation |
volume:39 firstpage:845 lastpage:851 numberofpages:7 journal:JOURNAL OF FISH DISEASES http://hdl.handle.net/11392/2337781 doi:10.1111/jfd.12418 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85027929694 http://www.blackwellpublishing.com/jfd |
op_doi |
https://doi.org/10.1111/jfd.12418 |
container_title |
Journal of Fish Diseases |
container_volume |
39 |
container_issue |
7 |
container_start_page |
845 |
op_container_end_page |
851 |
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1766396461276200960 |