The Antarctic krill Euphausia superba shows diurnal cycles of transcription under natural conditions
Background: Polar environments are characterized by extreme seasonal changes in day length, light intensity and spectrum, the extent of sea ice during the winter, and food availability. A key species of the Southern Ocean ecosystem, the Antarctic krill (Euphausia superba) has evolved rhythmic physio...
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Online Access: | https://hdl.handle.net/11392/1815702 https://doi.org/10.1371/journal.pone.0068652 https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0068652 https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0068652&type=printable |
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ftunivferrarair:oai:iris.unife.it:11392/1815702 2024-02-11T09:56:56+01:00 The Antarctic krill Euphausia superba shows diurnal cycles of transcription under natural conditions De Pittà C. Biscontin A. Albiero A. Sales G. Millino C. Mazzotta G. M. BERTOLUCCI, Cristiano Costa R. De Pittà, C. Biscontin, A. Albiero, A. Sales, G. Millino, C. Mazzotta, G. M. Bertolucci, Cristiano Costa, R. 2013 STAMPA https://hdl.handle.net/11392/1815702 https://doi.org/10.1371/journal.pone.0068652 https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0068652 https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0068652&type=printable eng eng info:eu-repo/semantics/altIdentifier/wos/WOS:000322000600013 volume:8 issue:7 firstpage:e68652-1 lastpage:e68652-11 numberofpages:11 journal:PLOS ONE https://hdl.handle.net/11392/1815702 doi:10.1371/journal.pone.0068652 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-84880429841 https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0068652 https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0068652&type=printable info:eu-repo/semantics/openAccess Antarctic Krill Euphausia superba Circadian rhythms info:eu-repo/semantics/article 2013 ftunivferrarair https://doi.org/10.1371/journal.pone.0068652 2024-01-17T17:35:01Z Background: Polar environments are characterized by extreme seasonal changes in day length, light intensity and spectrum, the extent of sea ice during the winter, and food availability. A key species of the Southern Ocean ecosystem, the Antarctic krill (Euphausia superba) has evolved rhythmic physiological and behavioral mechanisms to adapt to daily and seasonal changes. The molecular organization of the clockwork underlying these biological rhythms is, nevertheless, still only partially understood. Methodology/Principal Findings: The genome sequence of the Antarctic krill is not yet available. A normalized cDNA library was produced and pyrosequenced in the attempt to identify large numbers of transcripts. All available E. superba sequences were then assembled to create the most complete existing oligonucleotide microarray platform with a total of 32,217 probes. Gene expression signatures of specimens collected in the Ross Sea at five different time points over a 24- hour cycle were defined, and 1,308 genes differentially expressed were identified. Of the corresponding transcripts, 609 showed a significant sinusoidal expression pattern; about 40% of these exibithed a 24-hour periodicity while the other 60% was characterized by a shorter (about 12-hour) rhythm. We assigned the differentially expressed genes to functional categories and noticed that those concerning translation, proteolysis, energy and metabolic process, redox regulation, visual transduction and stress response, which are most likely related to daily environmental changes, were significantly enriched. Two transcripts of peroxiredoxin, thought to represent the ancestral timekeeping system that evolved about 2.5 billion years ago, were also identified as were two isoforms of the EsRh1 opsin and two novel arrestin1 sequences involved in the visual transduction cascade. Conclusions: Our work represents the first characterization of the krill diurnal transcriptome under natural conditions and provides a first insight into the genetic regulation of ... Article in Journal/Newspaper Antarc* Antarctic Antarctic Krill Euphausia superba Ross Sea Sea ice Southern Ocean Università degli Studi di Ferrara: CINECA IRIS Antarctic Ross Sea Southern Ocean The Antarctic PLoS ONE 8 7 e68652 |
institution |
Open Polar |
collection |
Università degli Studi di Ferrara: CINECA IRIS |
op_collection_id |
ftunivferrarair |
language |
English |
topic |
Antarctic Krill Euphausia superba Circadian rhythms |
spellingShingle |
Antarctic Krill Euphausia superba Circadian rhythms De Pittà C. Biscontin A. Albiero A. Sales G. Millino C. Mazzotta G. M. BERTOLUCCI, Cristiano Costa R. The Antarctic krill Euphausia superba shows diurnal cycles of transcription under natural conditions |
topic_facet |
Antarctic Krill Euphausia superba Circadian rhythms |
description |
Background: Polar environments are characterized by extreme seasonal changes in day length, light intensity and spectrum, the extent of sea ice during the winter, and food availability. A key species of the Southern Ocean ecosystem, the Antarctic krill (Euphausia superba) has evolved rhythmic physiological and behavioral mechanisms to adapt to daily and seasonal changes. The molecular organization of the clockwork underlying these biological rhythms is, nevertheless, still only partially understood. Methodology/Principal Findings: The genome sequence of the Antarctic krill is not yet available. A normalized cDNA library was produced and pyrosequenced in the attempt to identify large numbers of transcripts. All available E. superba sequences were then assembled to create the most complete existing oligonucleotide microarray platform with a total of 32,217 probes. Gene expression signatures of specimens collected in the Ross Sea at five different time points over a 24- hour cycle were defined, and 1,308 genes differentially expressed were identified. Of the corresponding transcripts, 609 showed a significant sinusoidal expression pattern; about 40% of these exibithed a 24-hour periodicity while the other 60% was characterized by a shorter (about 12-hour) rhythm. We assigned the differentially expressed genes to functional categories and noticed that those concerning translation, proteolysis, energy and metabolic process, redox regulation, visual transduction and stress response, which are most likely related to daily environmental changes, were significantly enriched. Two transcripts of peroxiredoxin, thought to represent the ancestral timekeeping system that evolved about 2.5 billion years ago, were also identified as were two isoforms of the EsRh1 opsin and two novel arrestin1 sequences involved in the visual transduction cascade. Conclusions: Our work represents the first characterization of the krill diurnal transcriptome under natural conditions and provides a first insight into the genetic regulation of ... |
author2 |
De Pittà, C. Biscontin, A. Albiero, A. Sales, G. Millino, C. Mazzotta, G. M. Bertolucci, Cristiano Costa, R. |
format |
Article in Journal/Newspaper |
author |
De Pittà C. Biscontin A. Albiero A. Sales G. Millino C. Mazzotta G. M. BERTOLUCCI, Cristiano Costa R. |
author_facet |
De Pittà C. Biscontin A. Albiero A. Sales G. Millino C. Mazzotta G. M. BERTOLUCCI, Cristiano Costa R. |
author_sort |
De Pittà C. |
title |
The Antarctic krill Euphausia superba shows diurnal cycles of transcription under natural conditions |
title_short |
The Antarctic krill Euphausia superba shows diurnal cycles of transcription under natural conditions |
title_full |
The Antarctic krill Euphausia superba shows diurnal cycles of transcription under natural conditions |
title_fullStr |
The Antarctic krill Euphausia superba shows diurnal cycles of transcription under natural conditions |
title_full_unstemmed |
The Antarctic krill Euphausia superba shows diurnal cycles of transcription under natural conditions |
title_sort |
antarctic krill euphausia superba shows diurnal cycles of transcription under natural conditions |
publishDate |
2013 |
url |
https://hdl.handle.net/11392/1815702 https://doi.org/10.1371/journal.pone.0068652 https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0068652 https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0068652&type=printable |
geographic |
Antarctic Ross Sea Southern Ocean The Antarctic |
geographic_facet |
Antarctic Ross Sea Southern Ocean The Antarctic |
genre |
Antarc* Antarctic Antarctic Krill Euphausia superba Ross Sea Sea ice Southern Ocean |
genre_facet |
Antarc* Antarctic Antarctic Krill Euphausia superba Ross Sea Sea ice Southern Ocean |
op_relation |
info:eu-repo/semantics/altIdentifier/wos/WOS:000322000600013 volume:8 issue:7 firstpage:e68652-1 lastpage:e68652-11 numberofpages:11 journal:PLOS ONE https://hdl.handle.net/11392/1815702 doi:10.1371/journal.pone.0068652 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-84880429841 https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0068652 https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0068652&type=printable |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1371/journal.pone.0068652 |
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PLoS ONE |
container_volume |
8 |
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7 |
container_start_page |
e68652 |
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