Immunocytochemical studies on the pituitary gland of Anguilla anguilla L., in relation to early growth stages and diet-induced sex differentiation
Mammalian and teleost antisera against pituitary hormones were used to identify and localize pituitary cell types in the European eel (Anguilla anguilla L.). The investigation was conducted on unpigmented glass eels of 5.6-6.2 of total body length (LT) caught in river mouths, then on yellow eels rea...
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ftunivferrarair:oai:iris.unife.it:11392/1198581 2024-02-11T09:55:30+01:00 Immunocytochemical studies on the pituitary gland of Anguilla anguilla L., in relation to early growth stages and diet-induced sex differentiation GRANDI, Gilberto COLOMBO, Giuseppe CHICCA, Milvia Grandi, Gilberto Colombo, Giuseppe Chicca, Milvia 2003 STAMPA http://hdl.handle.net/11392/1198581 https://doi.org/10.1016/S0016-6480(02)00646-9 eng eng info:eu-repo/semantics/altIdentifier/wos/WOS:000181578900009 volume:131 issue:1 firstpage:66 lastpage:76 journal:GENERAL AND COMPARATIVE ENDOCRINOLOGY http://hdl.handle.net/11392/1198581 doi:10.1016/S0016-6480(02)00646-9 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-0037336544 Pituitary gland Immunocytochemistry Secretory region change Induced feminization Adenohypophysis hormones info:eu-repo/semantics/article 2003 ftunivferrarair https://doi.org/10.1016/S0016-6480(02)00646-9 2024-01-24T17:34:22Z Mammalian and teleost antisera against pituitary hormones were used to identify and localize pituitary cell types in the European eel (Anguilla anguilla L.). The investigation was conducted on unpigmented glass eels of 5.6-6.2 of total body length (LT) caught in river mouths, then on yellow eels reared from the pigmented glass eel (or elvers) stage up to 12-14 cm of LT, in an eel farm in warm freshwater. Treated elvers were fed with commercial paste food supplemented with mature carp ovaries, containing oestradiol, that induced an early ovarian differentiation and a higher growth rate. The antisera detected seven types of immunoreactive (ir) cells, six of which were already found in glass eel adenohypophysis, suggesting differentiation of these cell types during the leptocephalus stage. In 12-14 cm treated yellow eels with small ovaries, a seventh type (ir-GtH) was detected in the proximal pars distalis; in the same animals the ir-TSH cells increased in number and size. From unpigmented glass eels to 12-14 cm yellow eels, the whole pituitary volume of controls increased nearly four times, while that of treated ones increased nearly six times. The larger volume of pituitary in treated eels was mainly due to volume increase of proximal pars distalis and rostral pars distalis. The %GH, that is the potential index of GH production, was significantly higher in treated yellow eels with gonads differentiating into ovaries than in controls; no difference was detected in %PRL between treated and control eels. The above results strongly suggest that in eels the feminizing effects of oestrogen is first exerted on the pituitary, probably through the hypothalamus, and later on the gonads. Article in Journal/Newspaper Anguilla anguilla Università degli Studi di Ferrara: CINECA IRIS General and Comparative Endocrinology 131 1 66 76 |
institution |
Open Polar |
collection |
Università degli Studi di Ferrara: CINECA IRIS |
op_collection_id |
ftunivferrarair |
language |
English |
topic |
Pituitary gland Immunocytochemistry Secretory region change Induced feminization Adenohypophysis hormones |
spellingShingle |
Pituitary gland Immunocytochemistry Secretory region change Induced feminization Adenohypophysis hormones GRANDI, Gilberto COLOMBO, Giuseppe CHICCA, Milvia Immunocytochemical studies on the pituitary gland of Anguilla anguilla L., in relation to early growth stages and diet-induced sex differentiation |
topic_facet |
Pituitary gland Immunocytochemistry Secretory region change Induced feminization Adenohypophysis hormones |
description |
Mammalian and teleost antisera against pituitary hormones were used to identify and localize pituitary cell types in the European eel (Anguilla anguilla L.). The investigation was conducted on unpigmented glass eels of 5.6-6.2 of total body length (LT) caught in river mouths, then on yellow eels reared from the pigmented glass eel (or elvers) stage up to 12-14 cm of LT, in an eel farm in warm freshwater. Treated elvers were fed with commercial paste food supplemented with mature carp ovaries, containing oestradiol, that induced an early ovarian differentiation and a higher growth rate. The antisera detected seven types of immunoreactive (ir) cells, six of which were already found in glass eel adenohypophysis, suggesting differentiation of these cell types during the leptocephalus stage. In 12-14 cm treated yellow eels with small ovaries, a seventh type (ir-GtH) was detected in the proximal pars distalis; in the same animals the ir-TSH cells increased in number and size. From unpigmented glass eels to 12-14 cm yellow eels, the whole pituitary volume of controls increased nearly four times, while that of treated ones increased nearly six times. The larger volume of pituitary in treated eels was mainly due to volume increase of proximal pars distalis and rostral pars distalis. The %GH, that is the potential index of GH production, was significantly higher in treated yellow eels with gonads differentiating into ovaries than in controls; no difference was detected in %PRL between treated and control eels. The above results strongly suggest that in eels the feminizing effects of oestrogen is first exerted on the pituitary, probably through the hypothalamus, and later on the gonads. |
author2 |
Grandi, Gilberto Colombo, Giuseppe Chicca, Milvia |
format |
Article in Journal/Newspaper |
author |
GRANDI, Gilberto COLOMBO, Giuseppe CHICCA, Milvia |
author_facet |
GRANDI, Gilberto COLOMBO, Giuseppe CHICCA, Milvia |
author_sort |
GRANDI, Gilberto |
title |
Immunocytochemical studies on the pituitary gland of Anguilla anguilla L., in relation to early growth stages and diet-induced sex differentiation |
title_short |
Immunocytochemical studies on the pituitary gland of Anguilla anguilla L., in relation to early growth stages and diet-induced sex differentiation |
title_full |
Immunocytochemical studies on the pituitary gland of Anguilla anguilla L., in relation to early growth stages and diet-induced sex differentiation |
title_fullStr |
Immunocytochemical studies on the pituitary gland of Anguilla anguilla L., in relation to early growth stages and diet-induced sex differentiation |
title_full_unstemmed |
Immunocytochemical studies on the pituitary gland of Anguilla anguilla L., in relation to early growth stages and diet-induced sex differentiation |
title_sort |
immunocytochemical studies on the pituitary gland of anguilla anguilla l., in relation to early growth stages and diet-induced sex differentiation |
publishDate |
2003 |
url |
http://hdl.handle.net/11392/1198581 https://doi.org/10.1016/S0016-6480(02)00646-9 |
genre |
Anguilla anguilla |
genre_facet |
Anguilla anguilla |
op_relation |
info:eu-repo/semantics/altIdentifier/wos/WOS:000181578900009 volume:131 issue:1 firstpage:66 lastpage:76 journal:GENERAL AND COMPARATIVE ENDOCRINOLOGY http://hdl.handle.net/11392/1198581 doi:10.1016/S0016-6480(02)00646-9 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-0037336544 |
op_doi |
https://doi.org/10.1016/S0016-6480(02)00646-9 |
container_title |
General and Comparative Endocrinology |
container_volume |
131 |
container_issue |
1 |
container_start_page |
66 |
op_container_end_page |
76 |
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1790596797423419392 |