Discovery and ecogenomic context of a global Caldiserica-related phylum active in thawing permafrost, Candidatus Cryosericota phylum nov., Ca. Cryosericia class nov., Ca. Cryosericales ord. nov., Ca. Cryosericaceae fam. nov., comprising the four species Cryosericum septentrionale gen. nov. sp. nov., Ca. C. hinesii sp. nov., Ca. C. odellii sp. nov., Ca. C. terrychapinii sp. nov.
This is the final version. Available on open access from Elsevier via the DOI in this record The phylum Caldiserica was identified from the hot spring 16S rRNA gene lineage ‘OP5’ and named for the sole isolate Caldisericum exile, a hot spring sulfur-reducing chemoheterotroph. Here we characterize 7...
Published in: | Systematic and Applied Microbiology |
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Online Access: | http://hdl.handle.net/10871/35678 https://doi.org/10.1016/j.syapm.2018.12.003 |
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ftunivexeter:oai:ore.exeter.ac.uk:10871/35678 2024-09-15T18:29:56+00:00 Discovery and ecogenomic context of a global Caldiserica-related phylum active in thawing permafrost, Candidatus Cryosericota phylum nov., Ca. Cryosericia class nov., Ca. Cryosericales ord. nov., Ca. Cryosericaceae fam. nov., comprising the four species Cryosericum septentrionale gen. nov. sp. nov., Ca. C. hinesii sp. nov., Ca. C. odellii sp. nov., Ca. C. terrychapinii sp. nov. Martinez, MA Woodcroft, BJ Ignacio Espinoza, JC Zayed, AA Singleton, CM Boyd, JA Li, YF Purvine, S Maughan, H Hodgkins, SB Anderson, D Sederholm, M Temperton, B Bolduc, B Saleska, SR Tyson, GW Rich, VI 2018 http://hdl.handle.net/10871/35678 https://doi.org/10.1016/j.syapm.2018.12.003 en eng Elsevier Published online 14 December 2018 doi:10.1016/j.syapm.2018.12.003 DE-SC0004632 DE-SC0010580 DE-SC0016440 49521 48467 http://hdl.handle.net/10871/35678 0723-2020 Systematic and Applied Microbiology © 2018. This version is made available under the CC-BY-NC-ND 4.0 license: https://creativecommons.org/licenses/by-nc-nd/4.0/ https://creativecommons.org/licenses/by-nc-nd/4.0/ Article 2018 ftunivexeter https://doi.org/10.1016/j.syapm.2018.12.003 2024-07-29T03:24:16Z This is the final version. Available on open access from Elsevier via the DOI in this record The phylum Caldiserica was identified from the hot spring 16S rRNA gene lineage ‘OP5’ and named for the sole isolate Caldisericum exile, a hot spring sulfur-reducing chemoheterotroph. Here we characterize 7 Caldiserica metagenome-assembled genomes (MAGs) from a thawing permafrost site in Stordalen Mire, Arctic Sweden. By 16S rRNA and marker gene phylogenies, and average nucleotide and amino acid identities, these Stordalen Mire Caldiserica (SMC) MAGs form part of a divergent clade from C. exile. Genome and meta-transcriptome and proteome analyses suggest that unlike Caldisericum, the SMCs (i) are carbohydrate- and possibly amino acid fermenters that can use labile plant compounds and peptides, and (ii) encode adaptations to low temperature. The SMC clade rose to community dominance within permafrost, with a peak metagenome-based relative abundance of ∼60%. It was also physiologically active in the upper seasonally-thawed soil. Beyond Stordalen Mire, analysis of 16S rRNA gene surveys indicated a global distribution of this clade, predominantly in anaerobic, carbon-rich and cold environments. These findings establish the SMCs as four novel phenotypically and ecologically distinct species within a single novel genus, distinct from C. exile clade at the phylum level. The SMCs are thus part of a novel cold-habitat phylum for an understudied, globally-distributed superphylum encompassing the Caldiserica. We propose the names Candidatus Cryosericota phylum nov., Ca. Cryosericia class nov., Ca. Cryosericales ord. nov., Ca. Cryosericaceae fam. nov., Ca. Cryosericum gen. nov., Ca. Cryosericum septentrionale sp. nov., Ca. C. hinesii sp. nov., Ca. C. odellii sp. nov., and Ca. C. terrychapinii sp. nov. United States Department of Energy Office of Biological and Environmental Research Environmental Molecular Sciences Laboratory (EMSL) Article in Journal/Newspaper permafrost University of Exeter: Open Research Exeter (ORE) Systematic and Applied Microbiology 42 1 54 66 |
institution |
Open Polar |
collection |
University of Exeter: Open Research Exeter (ORE) |
op_collection_id |
ftunivexeter |
language |
English |
description |
This is the final version. Available on open access from Elsevier via the DOI in this record The phylum Caldiserica was identified from the hot spring 16S rRNA gene lineage ‘OP5’ and named for the sole isolate Caldisericum exile, a hot spring sulfur-reducing chemoheterotroph. Here we characterize 7 Caldiserica metagenome-assembled genomes (MAGs) from a thawing permafrost site in Stordalen Mire, Arctic Sweden. By 16S rRNA and marker gene phylogenies, and average nucleotide and amino acid identities, these Stordalen Mire Caldiserica (SMC) MAGs form part of a divergent clade from C. exile. Genome and meta-transcriptome and proteome analyses suggest that unlike Caldisericum, the SMCs (i) are carbohydrate- and possibly amino acid fermenters that can use labile plant compounds and peptides, and (ii) encode adaptations to low temperature. The SMC clade rose to community dominance within permafrost, with a peak metagenome-based relative abundance of ∼60%. It was also physiologically active in the upper seasonally-thawed soil. Beyond Stordalen Mire, analysis of 16S rRNA gene surveys indicated a global distribution of this clade, predominantly in anaerobic, carbon-rich and cold environments. These findings establish the SMCs as four novel phenotypically and ecologically distinct species within a single novel genus, distinct from C. exile clade at the phylum level. The SMCs are thus part of a novel cold-habitat phylum for an understudied, globally-distributed superphylum encompassing the Caldiserica. We propose the names Candidatus Cryosericota phylum nov., Ca. Cryosericia class nov., Ca. Cryosericales ord. nov., Ca. Cryosericaceae fam. nov., Ca. Cryosericum gen. nov., Ca. Cryosericum septentrionale sp. nov., Ca. C. hinesii sp. nov., Ca. C. odellii sp. nov., and Ca. C. terrychapinii sp. nov. United States Department of Energy Office of Biological and Environmental Research Environmental Molecular Sciences Laboratory (EMSL) |
format |
Article in Journal/Newspaper |
author |
Martinez, MA Woodcroft, BJ Ignacio Espinoza, JC Zayed, AA Singleton, CM Boyd, JA Li, YF Purvine, S Maughan, H Hodgkins, SB Anderson, D Sederholm, M Temperton, B Bolduc, B Saleska, SR Tyson, GW Rich, VI |
spellingShingle |
Martinez, MA Woodcroft, BJ Ignacio Espinoza, JC Zayed, AA Singleton, CM Boyd, JA Li, YF Purvine, S Maughan, H Hodgkins, SB Anderson, D Sederholm, M Temperton, B Bolduc, B Saleska, SR Tyson, GW Rich, VI Discovery and ecogenomic context of a global Caldiserica-related phylum active in thawing permafrost, Candidatus Cryosericota phylum nov., Ca. Cryosericia class nov., Ca. Cryosericales ord. nov., Ca. Cryosericaceae fam. nov., comprising the four species Cryosericum septentrionale gen. nov. sp. nov., Ca. C. hinesii sp. nov., Ca. C. odellii sp. nov., Ca. C. terrychapinii sp. nov. |
author_facet |
Martinez, MA Woodcroft, BJ Ignacio Espinoza, JC Zayed, AA Singleton, CM Boyd, JA Li, YF Purvine, S Maughan, H Hodgkins, SB Anderson, D Sederholm, M Temperton, B Bolduc, B Saleska, SR Tyson, GW Rich, VI |
author_sort |
Martinez, MA |
title |
Discovery and ecogenomic context of a global Caldiserica-related phylum active in thawing permafrost, Candidatus Cryosericota phylum nov., Ca. Cryosericia class nov., Ca. Cryosericales ord. nov., Ca. Cryosericaceae fam. nov., comprising the four species Cryosericum septentrionale gen. nov. sp. nov., Ca. C. hinesii sp. nov., Ca. C. odellii sp. nov., Ca. C. terrychapinii sp. nov. |
title_short |
Discovery and ecogenomic context of a global Caldiserica-related phylum active in thawing permafrost, Candidatus Cryosericota phylum nov., Ca. Cryosericia class nov., Ca. Cryosericales ord. nov., Ca. Cryosericaceae fam. nov., comprising the four species Cryosericum septentrionale gen. nov. sp. nov., Ca. C. hinesii sp. nov., Ca. C. odellii sp. nov., Ca. C. terrychapinii sp. nov. |
title_full |
Discovery and ecogenomic context of a global Caldiserica-related phylum active in thawing permafrost, Candidatus Cryosericota phylum nov., Ca. Cryosericia class nov., Ca. Cryosericales ord. nov., Ca. Cryosericaceae fam. nov., comprising the four species Cryosericum septentrionale gen. nov. sp. nov., Ca. C. hinesii sp. nov., Ca. C. odellii sp. nov., Ca. C. terrychapinii sp. nov. |
title_fullStr |
Discovery and ecogenomic context of a global Caldiserica-related phylum active in thawing permafrost, Candidatus Cryosericota phylum nov., Ca. Cryosericia class nov., Ca. Cryosericales ord. nov., Ca. Cryosericaceae fam. nov., comprising the four species Cryosericum septentrionale gen. nov. sp. nov., Ca. C. hinesii sp. nov., Ca. C. odellii sp. nov., Ca. C. terrychapinii sp. nov. |
title_full_unstemmed |
Discovery and ecogenomic context of a global Caldiserica-related phylum active in thawing permafrost, Candidatus Cryosericota phylum nov., Ca. Cryosericia class nov., Ca. Cryosericales ord. nov., Ca. Cryosericaceae fam. nov., comprising the four species Cryosericum septentrionale gen. nov. sp. nov., Ca. C. hinesii sp. nov., Ca. C. odellii sp. nov., Ca. C. terrychapinii sp. nov. |
title_sort |
discovery and ecogenomic context of a global caldiserica-related phylum active in thawing permafrost, candidatus cryosericota phylum nov., ca. cryosericia class nov., ca. cryosericales ord. nov., ca. cryosericaceae fam. nov., comprising the four species cryosericum septentrionale gen. nov. sp. nov., ca. c. hinesii sp. nov., ca. c. odellii sp. nov., ca. c. terrychapinii sp. nov. |
publisher |
Elsevier |
publishDate |
2018 |
url |
http://hdl.handle.net/10871/35678 https://doi.org/10.1016/j.syapm.2018.12.003 |
genre |
permafrost |
genre_facet |
permafrost |
op_relation |
Published online 14 December 2018 doi:10.1016/j.syapm.2018.12.003 DE-SC0004632 DE-SC0010580 DE-SC0016440 49521 48467 http://hdl.handle.net/10871/35678 0723-2020 Systematic and Applied Microbiology |
op_rights |
© 2018. This version is made available under the CC-BY-NC-ND 4.0 license: https://creativecommons.org/licenses/by-nc-nd/4.0/ https://creativecommons.org/licenses/by-nc-nd/4.0/ |
op_doi |
https://doi.org/10.1016/j.syapm.2018.12.003 |
container_title |
Systematic and Applied Microbiology |
container_volume |
42 |
container_issue |
1 |
container_start_page |
54 |
op_container_end_page |
66 |
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1810471423672582144 |