Membrane-bound progesterone receptors in the canine uterus and placenta; possible targets in the maintenance of pregnancy

To date, the biological functions of P4 within the canine placenta have been attributed to maternal stroma-derived decidual cells as the only placental cells expressing the nuclear P4 receptor (PGR). However, P4 can also exert its effects via membrane-bound receptors. To test the hypothesis that mem...

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Published in:Theriogenology
Main Authors: Kazemian, A, Tavares Pereira, M, Aslan, S, Payan-Carreira, R, Reichler, I M, Agaoglu, R A, Kowalewski, M P
Format: Article in Journal/Newspaper
Language:Portuguese
Published: Elsevier 1483
Subjects:
Online Access:http://hdl.handle.net/10174/35684
https://doi.org/10.1016/j.theriogenology.2023.07.005
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spelling ftunivevora:oai:dspace.uevora.pt:10174/35684 2023-12-24T10:15:51+01:00 Membrane-bound progesterone receptors in the canine uterus and placenta; possible targets in the maintenance of pregnancy Kazemian, A Tavares Pereira, M Aslan, S Payan-Carreira, R Reichler, I M Agaoglu, R A Kowalewski, M P 2026-12 http://hdl.handle.net/10174/35684 https://doi.org/10.1016/j.theriogenology.2023.07.005 por por Elsevier Kazemian A, Tavares Pereira M, Aslan S, Payan-Carreira R, Reichler IM, Agaoglu RA, Kowalewski MP. Membrane-bound progesterone receptors in the canine uterus and placenta; possible targets in the maintenance of pregnancy. Theriogenology. 2023. 210:68-83. doi:10.1016/j.theriogenology.2023.07.005. https://doi.org/10.1016/j.theriogenology.2023.07.005 http://hdl.handle.net/10174/35684 nd rtpayan@uevora.pt 206 doi:10.1016/j.theriogenology.2023.07.005 openAccess Dog (Canis lupus familiaris) Placenta Membrane-bound P4 receptors Decidualization article 1483 ftunivevora https://doi.org/10.1016/j.theriogenology.2023.07.005 2023-11-24T00:12:38Z To date, the biological functions of P4 within the canine placenta have been attributed to maternal stroma-derived decidual cells as the only placental cells expressing the nuclear P4 receptor (PGR). However, P4 can also exert its effects via membrane-bound receptors. To test the hypothesis that membrane-bound P4 receptors are involved in regulating placental function in the dog, the expression of mPRα, -β, -γ, PGRMC1 and -2 was investigated in the uterine and placental compartments derived from different stages of pregnancy and from prepartum luteolysis. Further, to assess the PGR signaling-mediated effects upon membrane P4 receptors in canine decidual cells, in vitro decidualized dog uterine stromal (DUS) cells were treated with type II antigestagens (aglepristone or mifepristone). The expression of all membrane P4 receptors was detectable in reproductive tissues and in DUS cells. The main findings indicate their distinguishable placental spatio-temporal distribution; PGRMC2 was predominantly found in decidual cells, PGRMC1 was strong in maternal endothelial compartments, and syncytiotrophoblast showed abundant levels of mPRα and mPRβ. In vitro decidualization was associated with increased expression of PGRMC1 and -2, while their protein levels were diminished by antigestagen treatment. The involvement of membrane-bound P4 signaling in the regulation of canine placental function is implied, with P4 effects being directly exerted through maternal and fetal cellular compartments. The indirect effects of PGR might involve the modulation of membrane-bound receptors availability in decidual cells, implying a self-regulatory loop of P4 in regulating the availability of its own receptors in the canine placenta. Article in Journal/Newspaper Canis lupus Repositório Científico da Universidade de Évora Theriogenology 210 68 83
institution Open Polar
collection Repositório Científico da Universidade de Évora
op_collection_id ftunivevora
language Portuguese
topic Dog (Canis lupus familiaris)
Placenta
Membrane-bound P4 receptors
Decidualization
spellingShingle Dog (Canis lupus familiaris)
Placenta
Membrane-bound P4 receptors
Decidualization
Kazemian, A
Tavares Pereira, M
Aslan, S
Payan-Carreira, R
Reichler, I M
Agaoglu, R A
Kowalewski, M P
Membrane-bound progesterone receptors in the canine uterus and placenta; possible targets in the maintenance of pregnancy
topic_facet Dog (Canis lupus familiaris)
Placenta
Membrane-bound P4 receptors
Decidualization
description To date, the biological functions of P4 within the canine placenta have been attributed to maternal stroma-derived decidual cells as the only placental cells expressing the nuclear P4 receptor (PGR). However, P4 can also exert its effects via membrane-bound receptors. To test the hypothesis that membrane-bound P4 receptors are involved in regulating placental function in the dog, the expression of mPRα, -β, -γ, PGRMC1 and -2 was investigated in the uterine and placental compartments derived from different stages of pregnancy and from prepartum luteolysis. Further, to assess the PGR signaling-mediated effects upon membrane P4 receptors in canine decidual cells, in vitro decidualized dog uterine stromal (DUS) cells were treated with type II antigestagens (aglepristone or mifepristone). The expression of all membrane P4 receptors was detectable in reproductive tissues and in DUS cells. The main findings indicate their distinguishable placental spatio-temporal distribution; PGRMC2 was predominantly found in decidual cells, PGRMC1 was strong in maternal endothelial compartments, and syncytiotrophoblast showed abundant levels of mPRα and mPRβ. In vitro decidualization was associated with increased expression of PGRMC1 and -2, while their protein levels were diminished by antigestagen treatment. The involvement of membrane-bound P4 signaling in the regulation of canine placental function is implied, with P4 effects being directly exerted through maternal and fetal cellular compartments. The indirect effects of PGR might involve the modulation of membrane-bound receptors availability in decidual cells, implying a self-regulatory loop of P4 in regulating the availability of its own receptors in the canine placenta.
format Article in Journal/Newspaper
author Kazemian, A
Tavares Pereira, M
Aslan, S
Payan-Carreira, R
Reichler, I M
Agaoglu, R A
Kowalewski, M P
author_facet Kazemian, A
Tavares Pereira, M
Aslan, S
Payan-Carreira, R
Reichler, I M
Agaoglu, R A
Kowalewski, M P
author_sort Kazemian, A
title Membrane-bound progesterone receptors in the canine uterus and placenta; possible targets in the maintenance of pregnancy
title_short Membrane-bound progesterone receptors in the canine uterus and placenta; possible targets in the maintenance of pregnancy
title_full Membrane-bound progesterone receptors in the canine uterus and placenta; possible targets in the maintenance of pregnancy
title_fullStr Membrane-bound progesterone receptors in the canine uterus and placenta; possible targets in the maintenance of pregnancy
title_full_unstemmed Membrane-bound progesterone receptors in the canine uterus and placenta; possible targets in the maintenance of pregnancy
title_sort membrane-bound progesterone receptors in the canine uterus and placenta; possible targets in the maintenance of pregnancy
publisher Elsevier
publishDate 1483
url http://hdl.handle.net/10174/35684
https://doi.org/10.1016/j.theriogenology.2023.07.005
genre Canis lupus
genre_facet Canis lupus
op_relation Kazemian A, Tavares Pereira M, Aslan S, Payan-Carreira R, Reichler IM, Agaoglu RA, Kowalewski MP. Membrane-bound progesterone receptors in the canine uterus and placenta; possible targets in the maintenance of pregnancy. Theriogenology. 2023. 210:68-83. doi:10.1016/j.theriogenology.2023.07.005.
https://doi.org/10.1016/j.theriogenology.2023.07.005
http://hdl.handle.net/10174/35684
nd
rtpayan@uevora.pt
206
doi:10.1016/j.theriogenology.2023.07.005
op_rights openAccess
op_doi https://doi.org/10.1016/j.theriogenology.2023.07.005
container_title Theriogenology
container_volume 210
container_start_page 68
op_container_end_page 83
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